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Table of Contents

Volume 13 • Number 5 • October 2022
Current Issue

Letters to the Editor

BacteriologyLetter15 September 2022
New Phylum Names Harmonize Prokaryotic Nomenclature
  • Aharon Oren,
  • Markus Göker,
  • Iain C. Sutcliffe
    • on behalf of the Executive Board of the International Committee on Systematics of Prokaryotes
https://doi.org/10.1128/mbio.01479-22
We are pleased that Panda et al. (1) applaud the work of the International Committee on Systematics of Prokaryotes (ICSP) in bringing consistency to phylum names by voting in 2021 to include the rank of phylum in the International Code of Nomenclature of Prokaryotes (ICNP) (2). We here respond to the alleged pitfalls of the proposed nomenclatural system for naming phyla under the rules of the ICNP (3).
Clinical MicrobiologyLetter22 August 2022
Why Is It So Hard to Find Persistent Borreliella burgdorferi?
  • Steven W. Luger
https://doi.org/10.1128/mbio.02020-22
As a clinician since the early days of Lyme disease, I was eager to read the important article, “Borreliella burgdorferi antimicrobial-tolerant persistence in Lyme disease and posttreatment Lyme disease syndromes,” by F. C. Cabello et al. (1). Though most of the microbiology in the article was far beyond my comprehension, there are several clinical issues that I would like to address.

Commentaries

Antimicrobial ChemotherapyCommentary18 August 2022
Drug Resistance and Evolvability in an Emerging Human Fungal Pathogen
  • R. Blake Billmyre
https://doi.org/10.1128/mbio.01876-22
Over the past decade, Candida auris has emerged as a highly transmissible human fungal pathogen. Because of its ability to transmit between patients in hospitals and its ability to rapidly develop drug resistance, C. auris presents unique challenges.
MycologyCommentary12 September 2022
Central Sugar Metabolism and the Cell Wall
  • Hajar Yaakoub,
  • Jean-Paul Latgé,
  • Nicolas Papon
https://doi.org/10.1128/mbio.02104-22
The human opportunistic pathogen Aspergillus fumigatus is recognized for its versatile cell wall when it comes to remodeling its components in adaptation to external threats, and this remodeling renders it refractory to antifungals targeting cell wall ...
ImmunologyCommentary12 September 2022
Female Antibodies Go the Distance against Influenza Viruses
  • Marina R. Good,
  • Jenna J. Guthmiller
https://doi.org/10.1128/mbio.02106-22
Females have long been described to generate superior humoral immune responses relative to those in males. In the article by Ursin et al. (R. L. Ursin, S. Dhakal, H. Liu, S. Jayaraman, et al., mBio 13:e01839-22, 2022, https://doi.org/10.1128/mbio.01839-22)...
VirologyCommentary13 September 2022
Genetic Code Expansion and Bio-Orthogonal Labeling Reveal Intact HIV-1 Capsids inside the Nucleus
  • Leo C. James
https://doi.org/10.1128/mbio.02346-22
Microscopy is one of the few techniques that can directly observe the HIV-1 capsid as it traverses the cell. However, an extrinsic or intrinsic label is needed to facilitate detection and this can perturb capsid behavior.

Opinion/Hypothesis

VirologyOpinion/Hypothesis20 September 2022
Building a Resilient Scientific Network for COVID-19 and Beyond
  • N. Esther Babady,
  • Rachel M. Burckhardt,
  • Florian Krammer,
  • Penny L. Moore,
  • Lynn W. Enquist
https://doi.org/10.1128/mbio.02223-22
The continued evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) necessitates that the global scientific community monitor, assess, and respond to the evolving coronavirus disease (COVID-19) pandemic. But the current reactive ...

Minireviews

VirologyMinireview16 August 2022
The New SARS-CoV-2 Variants and Their Epidemiological Impact in Mexico
  • Rodrigo García-López,
  • Estibalitz Laresgoiti-Servitje,
  • Roselyn Lemus-Martin,
  • Alejandro Sanchez-Flores,
  • Carlos Sanders-Velez
https://doi.org/10.1128/mbio.01060-21
The COVID-19 disease caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus started its deadly journey into a global pandemic in Wuhan, China, in December 2019, where it was first isolated. Subsequently, multiple variants of the ...
Clinical MicrobiologyMinireview18 August 2022
Assessing the Cervicovaginal Microbiota in the Context of hrHPV Infections: Temporal Dynamics and Therapeutic Strategies
  • Mariano A. Molina,
  • Britt A. Coenen,
  • William P. J. Leenders,
  • Karolina M. Andralojc,
  • Martijn A. Huynen,
  • Willem J. G. Melchers
https://doi.org/10.1128/mbio.01619-22
Cervical cancer is the third leading cause of female cancers globally, resulting in more than 300,000 deaths every year. The majority of all cervical cancers are caused by persistent infections with high-risk human papillomaviruses (hrHPV) that can ...
Antimicrobial ChemotherapyMinireview26 September 2022
Canary in the Coal Mine: How Resistance Surveillance in Commensals Could Help Curb the Spread of AMR in Pathogenic Neisseria
  • Maira Goytia,
  • Crista B. Wadsworth
https://doi.org/10.1128/mbio.01991-22
Contemporary increases in the prevalence of antimicrobial resistance (AMR) in Neisseria gonorrhoeae populations is a direct threat to global public health and the effective treatment of gonorrhea. Substantial effort and financial support are being spent ...
VirologyMinireview18 August 2022
Decoding the Role of Temperature in RNA Virus Infections
  • Karishma Bisht,
  • Aartjan J. W. te Velthuis
https://doi.org/10.1128/mbio.02021-22
RNA viruses include respiratory viruses, such as coronaviruses and influenza viruses, as well as vector-borne viruses, like dengue and West Nile virus. RNA viruses like these encounter various environments when they copy themselves and spread from cell to ...

Observations

Microbial PathogenesisObservation08 September 2022
Protein Interaction Networks of Catalytically Active and Catalytically Inactive PqsE in Pseudomonas aeruginosa
  • Isabelle R. Taylor,
  • Laura A. Murray-Nerger,
  • Todd M. Greco,
  • Dawei Liu,
  • Ileana M. Cristea,
  • Bonnie L. Bassler
https://doi.org/10.1128/mbio.01559-22
Pseudomonas aeruginosa causes hospital-borne infections in vulnerable patients, including immunocompromised individuals, burn victims, and cancer patients undergoing chemotherapy. There are no effective treatments for P. aeruginosa infections, which are ...
Environmental MicrobiologyObservation31 August 2022
Symbiosis between Candidatus Patescibacteria and Archaea Discovered in Wastewater-Treating Bioreactors
  • Kyohei Kuroda,
  • Kyosuke Yamamoto,
  • Ryosuke Nakai,
  • Yuga Hirakata,
  • Kengo Kubota,
  • Masaru K. Nobu,
  • Takashi Narihiro
https://doi.org/10.1128/mbio.01711-22
One highly diverse phylogenetic group of Bacteria, Ca. Patescibacteria, remains poorly understood, but, from the few cultured representatives and metagenomic investigations, they are thought to live symbiotically or parasitically with other bacteria or ...
GeomicrobiologyObservation15 September 2022
Evidence for Quinol Oxidation Activity of ImoA, a Novel NapC/NirT Family Protein from the Neutrophilic Fe(II)-Oxidizing Bacterium Sideroxydans lithotrophicus ES-1
  • Abhiney Jain,
  • Anaísa Coelho,
  • Joana Madjarov,
  • Catarina M. Paquete,
  • Jeffrey A. Gralnick
https://doi.org/10.1128/mbio.02150-22
Fe(II)-oxidizing bacteria play an important role in biogeochemical cycles. At circumneutral pH, these organisms perform extracellular electron transfer, taking up electrons from Fe(II) outside the cell, potentially through a porin-cytochrome complex in ...

Research Articles

BacteriologyResearch Article26 September 2022
The Antibacterial Type VII Secretion System of Bacillus subtilis: Structure and Interactions of the Pseudokinase YukC/EssB
  • Matteo Tassinari,
  • Thierry Doan,
  • Marco Bellinzoni,
  • Maïalene Chabalier,
  • Mathilde Ben-Assaya,
  • Mariano Martinez,
  • Quentin Gaday,
  • Pedro M. Alzari,
  • Eric Cascales,
  • Rémi Fronzes,
  • Francesca Gubellini
https://doi.org/10.1128/mbio.00134-22
Type VII secretion systems mediate protein extrusion from Gram-positive bacteria and are classified as T7SSa and T7SSb in Actinobacteria and in Firmicutes, respectively. Despite the genetic divergence of T7SSa and T7SSb, the high degree of structural ...
VirologyResearch Article14 September 2022
Calpain-1: a Novel Antiviral Host Factor Identified in Porcine Small Intestinal Mucus
  • Yuchen Li,
  • Xiuyu Wang,
  • En Zhang,
  • Ruiling Liu,
  • Chengjie Yang,
  • Ying Duan,
  • Yuqi Jiang,
  • Qian Yang
https://doi.org/10.1128/mbio.00358-22
Although the antiviral activity of the intestinal mucus was recognized 20 years ago, the antiviral active ingredients in the mucus are poorly understood. Currently, most research on antiviral molecules in the intestinal mucus remains limited to members of ...
VirologyResearch Article17 August 2022
Emergence of Compensatory Mutations Reveals the Importance of Electrostatic Interactions between HIV-1 Integrase and Genomic RNA
  • Christian Shema Mugisha,
  • Tung Dinh,
  • Abhishek Kumar,
  • Kasyap Tenneti,
  • Jenna E. Eschbach,
  • Keanu Davis,
  • Robert Gifford,
  • Mamuka Kvaratskhelia,
  • Sebla B. Kutluay
https://doi.org/10.1128/mbio.00431-22
In addition to its catalytic function, HIV-1 integrase (IN) binds to the viral RNA genome (gRNA) through positively charged residues (i.e., R262, R263, R269, K273) within its C-terminal domain (CTD) and regulates proper virion maturation. Mutation of ...
Editor's PickGenetics and Molecular BiologyResearch Article16 August 2022
Barcoding Genetically Distinct Plasmodium falciparum Strains for Comparative Assessment of Fitness and Antimalarial Drug Resistance
  • Manuela Carrasquilla,
  • Ndey F. Drammeh,
  • Mukul Rawat,
  • Theo Sanderson,
  • Zenon Zenonos,
  • Julian C. Rayner,
  • Marcus C. S. Lee
https://doi.org/10.1128/mbio.00937-22
The complex interplay between antimalarial resistance and parasite fitness has important implications for understanding the development and spread of drug resistance alleles and the impact of genetic background on transmission. One limitation with current ...
BacteriologyResearch Article24 August 2022
Regulatory Interplay between RNase III and Antisense RNAs in E. coli: the Case of AsflhD and FlhD, Component of the Master Regulator of Motility
  • Maxence Lejars,
  • Joël Caillet,
  • Eugenio Solchaga-Flores,
  • Maude Guillier,
  • Jacqueline Plumbridge,
  • Eliane Hajnsdorf
https://doi.org/10.1128/mbio.00981-22
The role of antisense RNAs (asRNAs) in the regulation of gene expression remains largely unexplored in bacteria. Here, we confirm that asRNAs can be part of layered regulatory networks, since some are found opposite to genes encoding global regulators.
VirologyResearch Article06 September 2022
A Unique Role of the Human Cytomegalovirus Small Capsid Protein in Capsid Assembly
  • Eva Maria Borst,
  • Sarah Harmening,
  • Saskia Sanders,
  • Enrico Caragliano,
  • Karen Wagner,
  • Tihana Lenac Roviš,
  • Stipan Jonjić,
  • Jens Bernhard Bosse,
  • Martin Messerle
https://doi.org/10.1128/mbio.01007-22
Human cytomegalovirus (HCMV) is a ubiquitous pathogen causing life-threatening disease in immunocompromised individuals. Virus-specific processes such as capsid assembly and genome packaging can be exploited to design new antiviral strategies.
Host-Microbial InteractionsResearch Article07 September 2022
Bidirectional Interactions between Arboviruses and the Bacterial and Viral Microbiota in Aedes aegypti and Culex quinquefasciatus
  • Chenyan Shi,
  • Leen Beller,
  • Lanjiao Wang,
  • Ana Rosales Rosas,
  • Lander De Coninck,
  • Lyza Héry,
  • Laurence Mousson,
  • Nonito Pagès,
  • Jeroen Raes,
  • Leen Delang,
  • Anubis Vega-Rúa,
  • Anna-bella Failloux,
  • Jelle Matthijnssens
https://doi.org/10.1128/mbio.01021-22
In this study, we first utilized the single mosquito microbiome analysis, demonstrating a complex three-way interaction among arboviruses, resident microbiota, and the host, which is distinct for different arbovirus–mosquito combinations. Some of the ...
Editor's PickHost-Microbial InteractionsResearch Article30 August 2022
Motility-Independent Vertical Transmission of Bacteria in Leaf Symbiosis
  • Tessa Acar,
  • Sandra Moreau,
  • Olivier Coen,
  • Frédéric De Meyer,
  • Olivier Leroux,
  • Marine Beaumel,
  • Paul Wilkin,
  • Aurélien Carlier
https://doi.org/10.1128/mbio.01033-22
Hereditary symbioses with bacteria are common in the animal kingdom, but relatively unexplored in plants. Several plant species form associations with bacteria in their leaves, which is called leaf symbiosis.
ImmunologyResearch Article19 September 2022
High Glucose and Carbonyl Stress Impair HIF-1-Regulated Responses and the Control of Mycobacterium tuberculosis in Macrophages
  • Graciela Terán,
  • Hanxiong Li,
  • Sergiu-Bogdan Catrina,
  • Ruining Liu,
  • Susanna Brighenti,
  • Xiaowei Zheng,
  • Jakob Grünler,
  • Susanne Nylén,
  • Berit Carow,
  • Martin E. Rottenberg
https://doi.org/10.1128/mbio.01086-22
People living with diabetes who are also infected with M. tuberculosis are more likely to develop tuberculosis disease (TB). Why diabetic patients have an increased risk for developing TB is not well understood.
Genetics and Molecular BiologyResearch Article22 August 2022
Cobalamin Riboswitches Are Broadly Sensitive to Corrinoid Cofactors to Enable an Efficient Gene Regulatory Strategy
  • Kristopher J. Kennedy,
  • Florian J. Widner,
  • Olga M. Sokolovskaya,
  • Lina V. Innocent,
  • Rebecca R. Procknow,
  • Kenny C. Mok,
  • Michiko E. Taga
https://doi.org/10.1128/mbio.01121-22
Some bacterial mRNAs contain a region called a riboswitch which controls gene expression by binding to a metabolite in the cell. Typically, riboswitches sense and respond to a limited range of cellular metabolites, often just one type.
Host-Microbial InteractionsResearch Article29 August 2022
Community Dynamics in Structure and Function of Honey Bee Gut Bacteria in Response to Winter Dietary Shift
  • Chenyi Li,
  • Min Tang,
  • Xingan Li,
  • Xin Zhou
https://doi.org/10.1128/mbio.01131-22
The abilities to survive winter and to adapt to major food changes are key traits that have enabled successful range expansion of the honey bees from the tropic to temperate climate. Our results highlighted a new possibility that gut bacteria may have ...
Host-Microbial InteractionsResearch Article29 August 2022
CD55 Facilitates Immune Evasion by Borrelia crocidurae, an Agent of Relapsing Fever
  • Gunjan Arora,
  • Geoffrey E. Lynn,
  • Xiaotian Tang,
  • Connor E. Rosen,
  • Dieuwertje Hoornstra,
  • Andaleeb Sajid,
  • Joppe W. Hovius,
  • Noah W. Palm,
  • Aaron M. Ring,
  • Erol Fikrig
https://doi.org/10.1128/mbio.01161-22
Borrelia species are causative agents of Lyme disease and relapsing fever infections in humans. B. crocidurae causes one of the most prevalent relapsing fever infections in parts of West Africa. In the endemic regions, B. crocidurae is present in ~17% of ...
Antimicrobial ChemotherapyResearch Article03 October 2022
Cation ATPase (ATP4) Orthologue Replacement in the Malaria Parasite Plasmodium knowlesi Reveals Species-Specific Responses to ATP4-Targeting Drugs
  • Franziska Mohring,
  • Donelly A. van Schalkwyk,
  • Ryan C. Henrici,
  • Benjamin Blasco,
  • Didier Leroy,
  • Colin J. Sutherland,
  • Robert W. Moon
https://doi.org/10.1128/mbio.01178-22
Effective drugs are vital to minimize the illness and death caused by malaria. Development of new drugs becomes ever more urgent as drug resistance emerges.
Human MicrobiomeResearch Article08 September 2022
Evolution of the Gut Microbiome in HIV-Exposed Uninfected and Unexposed Infants during the First Year of Life
  • Conner L. Jackson,
  • Daniel N. Frank,
  • Charles E. Robertson,
  • Diana Ir,
  • Jennifer M. Kofonow,
  • Mahlodi P. Montlha,
  • Eleonora A. M. L. Mutsaerts,
  • Marta C. Nunes,
  • Shabir A. Madhi,
  • Debashis Ghosh,
  • Adriana Weinberg
https://doi.org/10.1128/mbio.01229-22
HIV-exposed uninfected infants (HEU) are highly vulnerable to infections in the first 6 months of life, and this vulnerability decreases to the age of 24 months. Because the microbiome plays a critical role in the education of the infant immune system, ...
Structural BiologyResearch Article29 August 2022
Atomic Structure of IglD Demonstrates Its Role as a Component of the Baseplate Complex of the Francisella Type VI Secretion System
  • Xiaoyu Liu,
  • Daniel L. Clemens,
  • Bai-Yu Lee,
  • Xue Yang,
  • Z. Hong Zhou,
  • Marcus A. Horwitz
https://doi.org/10.1128/mbio.01277-22
Francisella tularensis is a facultatively intracellular Gram-negative bacterium that causes the serious and potentially fatal zoonotic illness, tularemia. Because of its extraordinarily high infectivity and mortality to humans, especially when inhaled, F. ...
Editor's PickHost-Microbial InteractionsResearch Article07 September 2022
Intestinal Injury Biomarkers Predict Mortality in Pediatric Severe Malaria
  • Maithri L. Sarangam,
  • Ruth Namazzi,
  • Dibyadyuti Datta,
  • Caitlin Bond,
  • Charles P. B. Vanderpool,
  • Robert O. Opoka,
  • Chandy C. John,
  • Andrea L. Conroy
https://doi.org/10.1128/mbio.01325-22
In children with severe malaria, intestinal injury is a common complication associated with increased mortality. Intestinal injury is associated with acute kidney injury, acidosis, and endothelial activation. Interventions promoting intestinal ...
Editor's PickVirologyResearch Article12 September 2022
Rotavirus Downregulates Tyrosine Hydroxylase in the Noradrenergic Sympathetic Nervous System in Ileum, Early in Infection and Simultaneously with Increased Intestinal Transit and Altered Brain Activities
  • Arash Hellysaz,
  • Lennart Svensson,
  • Marie Hagbom
https://doi.org/10.1128/mbio.01387-22
Previous studies have investigated the mechanisms of rotavirus diarrhea mainly by focusing on intrinsic intestinal signaling. Although these observations are compelling and have provided important mechanistic information on rotavirus diarrhea, no ...
Systems BiologyResearch Article07 September 2022
Fatty Acid Synthesis Knockdown Promotes Biofilm Wrinkling and Inhibits Sporulation in Bacillus subtilis
  • Heidi A. Arjes,
  • Haiwen Gui,
  • Rachel Porter,
  • Esha Atolia,
  • Jason M. Peters,
  • Carol Gross,
  • Daniel B. Kearns,
  • Kerwyn Casey Huang
https://doi.org/10.1128/mbio.01388-22
For many bacteria, life typically involves growth in dense, three-dimensional communities called biofilms that contain cells with differentiated roles held together by extracellular matrix. To examine how essential gene function varies between vegetative ...
BacteriologyResearch Article07 September 2022
Dual Regulatory Role Exerted by Cyclic Dimeric GMP To Control FsnR-Mediated Bacterial Swimming
  • Xin Zhang,
  • Yan Wang,
  • Yao Wu,
  • Zhi-Hui Yuan,
  • Zhen Cai,
  • Wei Qian,
  • Xin Ge,
  • Fang-Fang Wang
https://doi.org/10.1128/mbio.01414-22
Stenotrophomonas maltophilia has become a great threat to human health because of the high mortality of infected patients. Swimming motility plays a crucial role in regulating bacterial virulence and adaptation.
BacteriologyResearch Article19 September 2022
Genome Capture Sequencing Selectively Enriches Bacterial DNA and Enables Genome-Wide Measurement of Intrastrain Genetic Diversity in Human Infections
  • Hillary S. Hayden,
  • Snehal Joshi,
  • Matthew C. Radey,
  • Anh T. Vo,
  • Cara Forsberg,
  • Sarah J. Morgan,
  • Adam Waalkes,
  • Elizabeth A. Holmes,
  • Sara M. Klee,
  • Mary J. Emond,
  • Pradeep K. Singh,
  • Stephen J. Salipante
https://doi.org/10.1128/mbio.01424-22
Genetic diversity evolves in bacterial strains during human infections and could affect disease manifestations and treatment resistance. However, the extent of diversity present in vivo and its changes over time are difficult to measure by conventional ...
Veterinary MicrobiologyResearch Article15 September 2022
Heat Shock Protein 60 Is Involved in Viral Replication Complex Formation and Facilitates Foot and Mouth Virus Replication by Stabilizing Viral Nonstructural Proteins 3A and 2C
  • Jianli Tang,
  • Sahibzada Waheed Abdullah,
  • Pinghua Li,
  • Jin'en Wu,
  • Chenchen Pei,
  • Suyu Mu,
  • Yunlu Wang,
  • Shiqi Sun,
  • Huichen Guo
https://doi.org/10.1128/mbio.01434-22
FMDV is the leading cause of the foot-and-mouth disease (FMD), affecting cloven-hoofed animals with high morbidity and mortality. We determined that HSP60 is required for efficient viral RNA replication and mRNA translation during FMDV infection.
Environmental MicrobiologyResearch Article25 August 2022
Toxin-Producing Endosymbionts Shield Pathogenic Fungus against Micropredators
  • Ingrid Richter,
  • Silvia Radosa,
  • Zoltán Cseresnyés,
  • Iuliia Ferling,
  • Hannah Büttner,
  • Sarah P. Niehs,
  • Ruman Gerst,
  • Kirstin Scherlach,
  • Marc Thilo Figge,
  • Falk Hillmann,
  • Christian Hertweck
https://doi.org/10.1128/mbio.01440-22
The soil community is a complex system characterized by predator-prey interactions. Fungi have developed effective strategies to defend themselves against predators.
Physiology and MetabolismResearch Article22 September 2022
Regulation of the Sae Two-Component System by Branched-Chain Fatty Acids in Staphylococcus aureus
  • Augustus Pendleton,
  • Won-Sik Yeo,
  • Shahad Alqahtani,
  • Dennis A. DiMaggio Jr.,
  • Carl J. Stone,
  • Zhaotao Li,
  • Vineet K. Singh,
  • Christopher P. Montgomery,
  • Taeok Bae,
  • Shaun R. Brinsmade
https://doi.org/10.1128/mbio.01472-22
Two-component systems (TCSs) are an essential way that bacteria sense and respond to their environment. These systems are usually composed of a membrane-bound histidine kinase that phosphorylates a cytoplasmic response regulator.
Applied and Industrial MicrobiologyResearch Article07 September 2022
Deciphering Cellodextrin and Glucose Uptake in Clostridium thermocellum
  • Fei Yan,
  • Sheng Dong,
  • Ya-Jun Liu,
  • Xingzhe Yao,
  • Chao Chen,
  • Yan Xiao,
  • Edward A. Bayer,
  • Yuval Shoham,
  • Chun You,
  • Qiu Cui,
  • Yingang Feng
https://doi.org/10.1128/mbio.01476-22
Highly efficient sugar uptake is important to microbial cell factories, and sugar transporters are therefore of great interest in the study of industrially relevant microorganisms. Clostridium thermocellum is a lignocellulolytic bacterium known for its ...
Genomics and ProteomicsResearch Article28 September 2022
Genetic Divergence of Vibrio vulnificus Clinical Isolates with Mild to Severe Outcomes
  • Kendall Kling,
  • Sonya A. Trinh,
  • Semen A. Leyn,
  • Dmitry A. Rodionov,
  • Ivan D. Rodionov,
  • Alfa Herrera,
  • Kasey Cervantes,
  • George Pankey,
  • Deborah Ashcraft,
  • Egon A. Ozer,
  • Adam Godzik,
  • Karla J. F. Satchell
https://doi.org/10.1128/mbio.01500-22
Vibrio vulnificus is an aquatic pathogen that is capable of causing severe disease in humans. Previous studies have suggested that pathogenic isolates were restricted to certain phylogenetic lineages and possibly toxinotype.
Microbial EcologyResearch Article20 September 2022
Uncovering Lasonolide A Biosynthesis Using Genome-Resolved Metagenomics
  • Siddharth Uppal,
  • Jackie L. Metz,
  • René K. M. Xavier,
  • Keshav Kumar Nepal,
  • Dongbo Xu,
  • Guojun Wang,
  • Jason C. Kwan
https://doi.org/10.1128/mbio.01524-22
While sponges are valuable sources of bioactive natural products, a majority of these compounds are produced in small quantities by uncultured symbionts, hampering the study and clinical development of these unique compounds. Lasonolide A (LSA), isolated ...
Host-Microbial InteractionsResearch Article20 September 2022
Symbiodiniaceae Are the First Site of Heterotrophic Nitrogen Assimilation in Reef-Building Corals
  • Stephane Martinez,
  • Renaud Grover,
  • David M. Baker,
  • Christine Ferrier-Pagès
https://doi.org/10.1128/mbio.01601-22
Coral reefs rely upon the highly optimized nutritional symbiosis between corals and Symbiodiniaceae dinoflagellates. Heterotrophic feeding on plankton is key to the resistance of corals to environmental stress.
Antimicrobial ChemotherapyResearch Article26 September 2022
Bacterial Membrane Vesicles as a Novel Strategy for Extrusion of Antimicrobial Bismuth Drug in Helicobacter pylori
  • Sumith Kumar,
  • Christine Schmitt,
  • Olivier Gorgette,
  • Martial Marbouty,
  • Magalie Duchateau,
  • Quentin Giai Gianetto,
  • Mariette Matondo,
  • Jean-Michel Guigner,
  • Hilde De Reuse
https://doi.org/10.1128/mbio.01633-22
Bacterial resistance to antibiotics is a major threat to human health. Treatments combining antibiotics with metals were proposed to circumvent this hurdle.
BacteriologyResearch Article06 October 2022
Bacterial Transcription Factors Bind to Coding Regions and Regulate Internal Cryptic Promoters
  • Canfeng Hua,
  • Jiadai Huang,
  • Tingting Wang,
  • Yue Sun,
  • Jingui Liu,
  • Linfeng Huang,
  • Xin Deng
https://doi.org/10.1128/mbio.01643-22
Although bacterial TFs regulate transcription by binding to specific sequences at the promoter region, little is known about the mechanisms and functions of TFs binding within the CDS. In this study, we show that bacterial TFs have same binding pattern in ...
ImmunologyResearch Article24 August 2022
Hybrid Immunity Shifts the Fc-Effector Quality of SARS-CoV-2 mRNA Vaccine-Induced Immunity
  • Kathryn A. Bowman,
  • Daniel Stein,
  • Sally Shin,
  • Kathie G. Ferbas,
  • Nicole H. Tobin,
  • Colin Mann,
  • Stephanie Fischinger,
  • Erica Ollmann Saphire,
  • Douglas Lauffenburger,
  • Anne W. Rimoin,
  • Grace Aldrovandi,
  • Galit Alter
https://doi.org/10.1128/mbio.01647-22
Recent data indicates improved immunity to SARS-CoV-2 in individuals who experience a combination of two mRNA vaccine doses and infection, “hybrid immunity,” compared to individuals who receive vaccination or experience infection alone. While previous ...
BacteriologyResearch Article26 September 2022
The pH Robustness of Bacterial Sensing
  • Elizabet Monteagudo-Cascales,
  • David Martín-Mora,
  • Wenhao Xu,
  • Victor Sourjik,
  • Miguel A. Matilla,
  • Álvaro Ortega,
  • Tino Krell
https://doi.org/10.1128/mbio.01650-22
Demonstration of the pH robustness of extracytoplasmic sensing reveals a previously undescribed evolutionary mechanism that enables bacteria to monitor environmental changes under changing conditions. This mechanism includes the maintenance of the dimeric ...
BacteriologyResearch Article14 September 2022
Transient Glycolytic Complexation of Arsenate Enhances Resistance in the Enteropathogen Vibrio cholerae
  • Emilio Bueno,
  • Víctor Pinedo,
  • Dhananjay D. Shinde,
  • André Mateus,
  • Athanasios Typas,
  • Mikhail M. Savitski,
  • Vinai C. Thomas,
  • Felipe Cava
https://doi.org/10.1128/mbio.01654-22
Even though resistance to arsenate has been extensively investigated in environmental bacteria, how enteric pathogens tolerate this toxic compound remains unknown. Here, we found that the cholera pathogen V. cholerae exhibits increased resistance to ...
Antimicrobial ChemotherapyResearch Article08 September 2022
Longitudinal Evolution of the Pseudomonas-Derived Cephalosporinase (PDC) Structure and Activity in a Cystic Fibrosis Patient Treated with β-Lactams
  • Claudia A. Colque,
  • Andrea G. albarracín Orio,
  • Pablo E. Tomatis,
  • Gina Dotta,
  • Diego M. Moreno,
  • Laura G. Hedemann,
  • Rachel A. Hickman,
  • Lea M. Sommer,
  • Sofía Feliziani,
  • Alejandro J. Moyano,
  • Robert A. Bonomo,
  • Helle K. Johansen,
  • Søren Molin,
  • Alejandro J. Vila,
  • Andrea M. Smania
https://doi.org/10.1128/mbio.01663-22
Antibiotics are becoming increasingly ineffective to treat bacterial infections. It has been consequently predicted that infectious diseases will become the biggest challenge to human health in the near future. Pseudomonas aeruginosa is considered a ...
VirologyResearch Article30 August 2022
Three-Layered Complex Interactions among Capsidless (+)ssRNA Yadokariviruses, dsRNA Viruses, and a Fungus
  • Yukiyo Sato,
  • Sakae Hisano,
  • Carlos José López-Herrera,
  • Hideki Kondo,
  • Nobuhiro Suzuki
https://doi.org/10.1128/mbio.01685-22
A capsidless (+)ssRNA virus YkV1 (family Yadokariviridae) highjacks the capsid of an unrelated dsRNA virus YnV1 (proposed family “Yadonushiviridae”) in a phytopathogenic ascomycete, while YkV1 trans-enhances YnV1 replication. Herein, we identified the ...
Molecular and Cellular BiologyResearch Article06 September 2022
Genetic Screens Identify Additional Genes Implicated in Envelope Remodeling during the Engulfment Stage of Bacillus subtilis Sporulation
  • Helena Chan,
  • Najwa Taib,
  • Michael C. Gilmore,
  • Ahmed M. T. Mohamed,
  • Kieran Hanna,
  • Johana Luhur,
  • Hieu Nguyen,
  • Elham Hafiz,
  • Felipe Cava,
  • Simonetta Gribaldo,
  • David Rudner,
  • Christopher D. A. Rodrigues
https://doi.org/10.1128/mbio.01732-22
In bacteria, cell envelope remodeling is critical for cell growth and division. This is also the case during the development of bacteria into highly resistant endospores (spores), known as sporulation.
Microbial PathogenesisResearch Article29 August 2022
Plasmodium falciparum and TNF-α Differentially Regulate Inflammatory and Barrier Integrity Pathways in Human Brain Endothelial Cells
  • Marisol Zuniga,
  • Claudia Gomes,
  • Ze Chen,
  • Criseyda Martinez,
  • Joseph Cooper Devlin,
  • P’ng Loke,
  • Ana Rodriguez
https://doi.org/10.1128/mbio.01746-22
Cerebral malaria is a severe complication of Plasmodium falciparum infection that causes the loss of blood-brain barrier integrity and frequently results in death. Here, we compared the effect of P. falciparum-infected red blood cells and inflammatory ...
ImmunologyResearch Article22 September 2022
Outcomes of Convalescent Plasma with Defined High versus Lower Neutralizing Antibody Titers against SARS-CoV-2 among Hospitalized Patients: CoronaVirus Inactivating Plasma (CoVIP) Study
  • Luther A. Bartelt,
  • Alena J. Markmann,
  • Bridget Nelson,
  • Jessica Keys,
  • Heather Root,
  • Heather I. Henderson,
  • JoAnn Kuruc,
  • Caroline Baker,
  • D. Ryan Bhowmik,
  • Yixuan J. Hou,
  • Lakshmanane Premkumar,
  • Caleb Cornaby,
  • John L. Schmitz,
  • Susan Weiss,
  • Yara Park,
  • Ralph Baric,
  • Aravinda M. de Silva,
  • Anne Lachiewicz,
  • Sonia Napravnik,
  • David van Duin,
  • David M. Margolis
https://doi.org/10.1128/mbio.01751-22
In this study, in a high-risk population of patients admitted for COVID-19, we found an earlier time to hospital discharge among participants receiving CCP with nAb titers of >1:640 compared with participants receiving CCP with a lower nAb titer and no ...
Editor's PickEukaryotic CellsResearch Article19 September 2022
Systematic Analysis of Copy Number Variations in the Pathogenic Yeast Candida parapsilosis Identifies a Gene Amplification in RTA3 That is Associated with Drug Resistance
  • Sean A. Bergin,
  • Fang Zhao,
  • Adam P. Ryan,
  • Carolin A. Müller,
  • Conrad A. Nieduszynski,
  • Bing Zhai,
  • Thierry Rolling,
  • Tobias M. Hohl,
  • Florent Morio,
  • Jillian Scully,
  • Kenneth H. Wolfe,
  • Geraldine Butler
https://doi.org/10.1128/mbio.01777-22
Copy number variations (CNVs) are an important source of genomic diversity that have been associated with drug resistance. We identify two unusual CNVs in the human fungal pathogen Candida parapsilosis.
BacteriologyResearch Article07 September 2022
The Group B Streptococcal Adhesin BspC Interacts with Host Cytokeratin 19 To Promote Colonization of the Female Reproductive Tract
  • Haider S. Manzer,
  • Dustin T. Nguyen,
  • Joo Youn Park,
  • Nogi Park,
  • Keun Seok Seo,
  • Justin A. Thornton,
  • Angela H. Nobbs,
  • Kelly S. Doran
https://doi.org/10.1128/mbio.01781-22
Group B Streptococcus (GBS) asymptomatically colonizes the female reproductive tract (FRT) of up to one third of women, but GBS carriage can lead to adverse pregnancy outcomes, including premature rupture of membranes, preterm labor, and chorioamnionitis. ...
VirologyResearch Article23 August 2022
SARS-CoV-2 Spike-Binding Antibody Longevity and Protection from Reinfection with Antigenically Similar SARS-CoV-2 Variants
  • John Kubale,
  • Charles Gleason,
  • Juan Manuel Carreño,
  • Komal Srivastava,
  • Gagandeep Singh,
  • PARIS Study Team,
  • Aubree Gordon,
  • Florian Krammer,
  • Viviana Simon
https://doi.org/10.1128/mbio.01784-22
SARS-CoV-2 is the cause of one of the largest noninfluenza pandemics of this century. This exceptional public health crisis highlights the urgent need for better understanding of the correlates of protection from infection and severe COVID-19.
Environmental MicrobiologyResearch Article30 August 2022
N-Succinyltransferase Encoded by a Cryptic Siderophore Biosynthesis Gene Cluster in Streptomyces Modifies Structurally Distinct Antibiotics
  • Olha Schneider,
  • Martin Zehl,
  • Reiko Ueoka,
  • Christian Rückert,
  • Tobias Buche,
  • Yi Jiang,
  • Qin-Yuan Li,
  • Jörn Piel,
  • Sergey B. Zotchev
https://doi.org/10.1128/mbio.01789-22
In their natural habitats, bacteria encounter a plethora of organic compounds, some of which may be represented by antibiotics produced by certain members of the microbial community. A number of antibiotic resistance mechanisms have been described, ...
Microbial PathogenesisResearch Article22 September 2022
Salmonella enterica Infections Are Disrupted by Two Small Molecules That Accumulate within Phagosomes and Differentially Damage Bacterial Inner Membranes
  • Joseph A. Villanueva,
  • Amy L. Crooks,
  • Toni A. Nagy,
  • Joaquin L. J. Quintana,
  • Zachary D. Dalebroux,
  • Corrella S. Detweiler
https://doi.org/10.1128/mbio.01790-22
Innovative strategies for developing new antimicrobials are needed. Combining our knowledge of host-pathogen interactions and relevant drug characteristics has the potential to reveal new approaches to treating infection.
MycologyResearch Article24 August 2022
Orchestrated Biosynthesis of the Secondary Metabolite Cocktails Enables the Producing Fungus to Combat Diverse Bacteria
  • Yanlei Sun,
  • Bo Chen,
  • Xinlin Li,
  • Ying Yin,
  • Chengshu Wang
https://doi.org/10.1128/mbio.01800-22
Fungal chemical ecology is largely mediated by the metabolite(s) produced by individual biosynthetic gene clusters (BGCs) with antibiotic activities. We report a supercluster containing three BGCs that are jointly controlled by an embedded master ...
VirologyResearch Article03 October 2022
Structural and Mechanistic Bases of Viral Resistance to HIV-1 Capsid Inhibitor Lenacapavir
  • Stephanie M. Bester,
  • Daniel Adu-Ampratwum,
  • Arun S. Annamalai,
  • Guochao Wei,
  • Lorenzo Briganti,
  • Bridget C. Murphy,
  • Reed Haney,
  • James R. Fuchs,
  • Mamuka Kvaratskhelia
https://doi.org/10.1128/mbio.01804-22
LEN is an investigational long-acting agent for future HIV-1 treatment regimens. While ongoing clinical trials have highlighted a largely beneficial profile of LEN for the treatment of HIV-1 infected people with limited therapy options, one notable ...
Molecular and Cellular BiologyResearch Article30 August 2022
TurboID Identification of Evolutionarily Divergent Components of the Nuclear Pore Complex in the Malaria Model Plasmodium berghei
  • Sushma V. Ambekar,
  • Josh R. Beck,
  • Gunnar R. Mair
https://doi.org/10.1128/mbio.01815-22
The nuclear pore complex (NPC) is a platform for constant evolution and has been used to study the evolutionary patterns of early-branching eukaryotes. The Plasmodium NPC is poorly defined due to its evolutionary divergent nature making it impossible to ...
BacteriologyResearch Article24 August 2022
Biochemical and Structural Study of RuvC and YqgF from Deinococcus radiodurans
  • Yiyang Sun,
  • Jieyu Yang,
  • Guangzhi Xu,
  • Kaiying Cheng
https://doi.org/10.1128/mbio.01834-22
Deinococcus radiodurans is one of the most radioresistant bacteria in the world due to its robust DNA damage response and repair abilities, which are contributed by its efficient homologous recombination repair system. However, the late steps of ...
Host-Microbial InteractionsResearch Article31 August 2022
Plakoglobin and High-Mobility Group Box 1 Mediate Intestinal Epithelial Cell Apoptosis Induced by Clostridioides difficile TcdB
  • Yingxue Li,
  • Wei Xu,
  • Yutian Ren,
  • Hung-Chi Cheung,
  • Panpan Huang,
  • Guneet Kaur,
  • Chih-Jung Kuo,
  • Sean P. McDonough,
  • Susan L. Fubini,
  • Stephen M. Lipkin,
  • Xin Deng,
  • Yung-Fu Chang,
  • Linfeng Huang
https://doi.org/10.1128/mbio.01849-22
Clostridioides difficile infection (CDI) is the leading cause of hospital-acquired diarrhea. Toxins, especially TcdB, cause epithelial cell apoptosis, but the underlying cell death mechanism is less clear.
Clinical MicrobiologyResearch Article26 September 2022
Phage-Plasmids Spread Antibiotic Resistance Genes through Infection and Lysogenic Conversion
  • Eugen Pfeifer,
  • Rémy A. Bonnin,
  • Eduardo P. C. Rocha
https://doi.org/10.1128/mbio.01851-22
The dissemination of antimicrobial resistance is a major threat to global health. Here, we show that a group of temperate bacterial viruses (phages), termed phage-plasmids, commonly encode different and multiple types of resistance genes of high clinical ...
BacteriologyResearch Article26 September 2022
Tag-Dependent Substrate Selection of ClpX Underlies Secondary Differentiation of Chlamydia trachomatis
  • Nicholas A. Wood,
  • Abigail R. Swoboda,
  • Amanda M. Blocker,
  • Derek J. Fisher,
  • Scot P. Ouellette
https://doi.org/10.1128/mbio.01858-22
Chlamydia trachomatis is the leading cause of bacterial sexually transmitted infections and preventable infectious blindness. This unique organism undergoes developmental transitions between infectious, nondividing forms and noninfectious, dividing forms.
Eukaryotic CellsResearch Article07 September 2022
Separate To Operate: the Centriole-Free Inner Core of the Centrosome Regulates the Assembly of the Intranuclear Spindle in Toxoplasma gondii
  • Ramiro Tomasina,
  • Fabiana C. Gonzalez,
  • Érica S. Martins-Duarte,
  • Philippe Bastin,
  • Mathieu Gissot,
  • María E. Francia
https://doi.org/10.1128/mbio.01859-22
Toxoplasma gondii parasites cause toxoplasmosis, arguably the most widespread and prevalent parasitosis of humans and animals. During the clinically relevant stage of its life cycle, the parasites divide by endodyogeny.
VirologyResearch Article14 September 2022
Immune Imprinting Drives Human Norovirus Potential for Global Spread
  • Lisa C. Lindesmith,
  • Florencia A. T. Boshier,
  • Paul D. Brewer-Jensen,
  • Sunando Roy,
  • Veronica Costantini,
  • Michael L. Mallory,
  • Mark Zweigart,
  • Samantha R. May,
  • Helen Conrad,
  • Kathleen M. O’Reilly,
  • Daniel Kelly,
  • Cristina C. Celma,
  • Stuart Beard,
  • Rachel Williams,
  • Helena J. Tutill,
  • Sylvia Becker Dreps,
  • Filemón Bucardo,
  • David J. Allen,
  • Jan Vinjé,
  • Richard A. Goldstein,
  • Judith Breuer,
  • Ralph S. Baric
https://doi.org/10.1128/mbio.01861-22
In our model, preepidemic human norovirus variants harbor genetic diversification that translates into novel antigenic features without compromising viral fitness. Through surveillance, we identified two viruses fitting this profile, forming long branches ...
Genetics and Molecular BiologyResearch Article14 September 2022
Uncovering Cis-Regulatory Elements Important for A-to-I RNA Editing in Fusarium graminearum
  • Chanjing Feng,
  • Xinyu Cao,
  • Yanfei Du,
  • Yitong Chen,
  • Kaiyun Xin,
  • Jingwen Zou,
  • Qiaojun Jin,
  • Jin-Rong Xu,
  • Huiquan Liu
https://doi.org/10.1128/mbio.01872-22
A-to-I RNA editing is a new epigenetic phenomenon that is crucial for sexual reproduction in fungi. Deciphering cis-regulatory elements of A-to-I RNA editing can help us elucidate the editing mechanism and develop a model that accurately predicts RNA ...
Genetics and Molecular BiologyResearch Article18 August 2022
A Conundrum of r-Protein Stability: Unbalanced Stoichiometry of r-Proteins during Stationary Phase in Escherichia coli
  • Kaspar Reier,
  • Petri-Jaan Lahtvee,
  • Aivar Liiv,
  • Jaanus Remme
https://doi.org/10.1128/mbio.01873-22
Ribosome degradation has been extensively described from the viewpoint of its main component, rRNA. Here, we aim to complement our knowledge by quantitatively analyzing r-protein degradation and stability both in the ribosomes and in the whole-cell ...
Microbial PathogenesisResearch Article29 August 2022
Dysfunctional Glymphatic System with Disrupted Aquaporin 4 Expression Pattern on Astrocytes Causes Bacterial Product Accumulation in the CSF during Pneumococcal Meningitis
  • Jaqueline S. Generoso,
  • Sigrun Thorsdottir,
  • Allan Collodel,
  • Diogo Dominguini,
  • Roberta R. E. Santo,
  • Fabricia Petronilho,
  • Tatiana Barichello,
  • Federico Iovino
https://doi.org/10.1128/mbio.01886-22
The lack of solute drainage due to a dysfunctional glymphatic system leads to an increase of the neurotoxic bacterial material in the CSF compartments of the brain, ultimately leading to brain-wide neuroinflammation and neuronal damage with consequent ...
Host-Microbial InteractionsResearch Article26 September 2022
Interferon-Inducible E3 Ligase RNF213 Facilitates Host-Protective Linear and K63-Linked Ubiquitylation of Toxoplasma gondii Parasitophorous Vacuoles
  • Dulcemaria Hernandez,
  • Stephen Walsh,
  • Luz Saavedra Sanchez,
  • Mary S. Dickinson,
  • Jörn Coers
https://doi.org/10.1128/mbio.01888-22
Globally, approximately one out of three people become infected with the obligate intracellular parasite Toxoplasma. These infections are typically asymptomatic but can cause severe disease and mortality in immunocompromised individuals. Infections can ...
VirologyResearch Article08 September 2022
CCL2: a Chemokine Potentially Promoting Early Seeding of the Latent HIV Reservoir
  • Thomas A. Packard,
  • Roland Schwarzer,
  • Eytan Herzig,
  • Deepashri Rao,
  • Xiaoyu Luo,
  • Johanne H. Egedal,
  • Feng Hsiao,
  • Marek Widera,
  • Judd F. Hultquist,
  • Zachary W. Grimmett,
  • Ronald J. Messer,
  • Nevan J. Krogan,
  • Steven G. Deeks,
  • Nadia R. Roan,
  • Ulf Dittmer,
  • Kim J. Hasenkrug,
  • Warner C. Greene
https://doi.org/10.1128/mbio.01891-22
There are currently over 35 million people living with HIV worldwide, and we still have no vaccine or scalable cure. One of the difficulties with HIV is its ability to rapidly establish a viral reservoir in lymphoid tissues that allows it to elude ...
Genomics and ProteomicsResearch Article12 September 2022
Persistence of Rare Salmonella Typhi Genotypes Susceptible to First-Line Antibiotics in the Remote Islands of Samoa
  • Michael J. Sikorski,
  • Tracy H. Hazen,
  • Sachin N. Desai,
  • Susana Nimarota-Brown,
  • Siaosi Tupua,
  • Michelle Sialeipata,
  • Savitra Rambocus,
  • Danielle J. Ingle,
  • Sebastian Duchene,
  • Susan A. Ballard,
  • Mary Valcanis,
  • Sara Zufan,
  • Jianguo Ma,
  • Jason W. Sahl,
  • Mailis Maes,
  • Gordon Dougan,
  • Robert E. Thomsen,
  • Roy M. Robins-Browne,
  • Benjamin P. Howden,
  • Take K. Naseri,
  • Myron M. Levine,
  • David A. Rasko
https://doi.org/10.1128/mbio.01920-22
In this study, we used whole genome sequencing and comparative genomics analyses to characterize the population structure, evolutionary origins, and genomic features of S. Typhi associated with decades of endemic typhoid fever in Samoa.
BacteriologyResearch Article08 September 2022
Redox-Mediated Inactivation of the Transcriptional Repressor RcrR is Responsible for Uropathogenic Escherichia coli’s Increased Resistance to Reactive Chlorine Species
  • Sadia Sultana,
  • Mary E. Crompton,
  • Kennadi Meurer,
  • Olivia Jankiewicz,
  • Grace H. Morales,
  • Colton Johnson,
  • Elise Horbach,
  • Kevin Pierre Hoffmann,
  • Pooja Kr,
  • Ritika Shah,
  • Greg M. Anderson,
  • Nathan T. Mortimer,
  • Jonathan E. Schmitz,
  • Maria Hadjifrangiskou,
  • Alessandro Foti,
  • Jan-Ulrik Dahl
https://doi.org/10.1128/mbio.01926-22
How do pathogens deal with antimicrobial oxidants produced by the innate immune system during infection? Uropathogenic Escherichia coli (UPEC), the most common etiological agent of urinary tract infections (UTIs), is particularly exposed to infiltrating ...
BacteriologyResearch Article08 September 2022
Genetic Determinants of Hydrogen Sulfide Biosynthesis in Fusobacterium nucleatum Are Required for Bacterial Fitness, Antibiotic Sensitivity, and Virulence
  • Yi-Wei Chen,
  • Martha I. Camacho,
  • Yimin Chen,
  • Aadil H. Bhat,
  • Chungyu Chang,
  • Emily A. Peluso,
  • Chenggang Wu,
  • Asis Das,
  • Hung Ton-That
https://doi.org/10.1128/mbio.01936-22
Fusobacterium nucleatum is a key commensal anaerobe of the human oral cavity that plays a significant role in oral biofilm development and contributes to additional pathologies at extraoral sites, such as promoting preterm birth and colorectal cancer. ...
MycologyResearch Article22 August 2022
Collaboration between Antagonistic Cell Type Regulators Governs Natural Variation in the Candida albicans Biofilm and Hyphal Gene Expression Network
  • Eunsoo Do,
  • Max V. Cravener,
  • Manning Y. Huang,
  • Gemma May,
  • C. Joel McManus,
  • Aaron P. Mitchell
https://doi.org/10.1128/mbio.01937-22
Clinical isolates of all pathogens vary in the strength of traits linked to disease. In this study, we focused on variation in a pathogenicity trait of the fungal pathogen Candida albicans, biofilm formation.
BacteriologyResearch Article08 September 2022
RcgA and RcgR, Two Novel Proteins Involved in the Conjugative Transfer of Rhizobial Plasmids
  • Lucas G. Castellani,
  • Abril Luchetti,
  • Juliet F. Nilsson,
  • Julieta Pérez-Giménez,
  • Ben Struck,
  • Andreas Schlüter,
  • Alfred Pühler,
  • Karsten Niehaus,
  • David Romero,
  • Mariano Pistorio,
  • Gonzalo Torres Tejerizo
https://doi.org/10.1128/mbio.01949-22
Extrachromosomal DNA elements, such as plasmids, allow for the adaptation of bacteria to new environments by conferring new determinants. Via conjugation, plasmids can be transferred between members of the same bacterial species, different species, or ...
Microbial GeneticsResearch Article17 August 2022
Histone Acetyltransferase CfGcn5-Mediated Autophagy Governs the Pathogenicity of Colletotrichum fructicola
  • Shengpei Zhang,
  • Yuan Guo,
  • Sizheng Li,
  • He Li
https://doi.org/10.1128/mbio.01956-22
Colletotrichum spp. is ranked in the top 10 plant fungal pathogens and serves as a model for the study of hemibiotrophic pathogens, but its molecular mechanisms of pathogenesis remain largely unknown. Among species of Colletotrichum, C. fructicola causes ...
Editor's PickVirologyResearch Article16 August 2022
Direct Capsid Labeling of Infectious HIV-1 by Genetic Code Expansion Allows Detection of Largely Complete Nuclear Capsids and Suggests Nuclear Entry of HIV-1 Complexes via Common Routes
  • Sandra Schifferdecker,
  • Vojtech Zila,
  • Thorsten G. Müller,
  • Volkan Sakin,
  • Maria Anders-Össwein,
  • Vibor Laketa,
  • Hans-Georg Kräusslich,
  • Barbara Müller
https://doi.org/10.1128/mbio.01959-22
The cone-shaped capsid of HIV-1 has recently been recognized as a master organizer of events from cell entry of the virus to the integration of the viral genome into the host cell DNA. Fluorescent labeling of the capsid is essential to study its role in ...
ParasitologyResearch Article06 October 2022
A Signaling Factor Linked to Toxoplasma gondii Guanylate Cyclase Complex Controls Invasion and Egress during Acute and Chronic Infection
  • Shu Ye,
  • Matteo Lunghi,
  • Dominique Soldati-Favre
https://doi.org/10.1128/mbio.01965-22
Toxoplasma gondii is an obligate intracellular parasite of the phylum Apicomplexa. This life-threatening opportunistic pathogen establishes a chronic infection in human and animals that is resistant to immune attacks and chemotherapeutic intervention.
Antimicrobial ChemotherapyResearch Article21 September 2022
The Heptaprenyl Diphosphate Synthase (Coq1) Is the Target of a Lipophilic Bisphosphonate That Protects Mice against Toxoplasma gondii Infection
  • Melissa A. Sleda,
  • Zhu-Hong Li,
  • Ranjan Behera,
  • Baihetiya Baierna,
  • Catherine Li,
  • Jomkwan Jumpathong,
  • Satish R. Malwal,
  • Makoto Kawamukai,
  • Eric Oldfield,
  • Silvia N. J. Moreno
https://doi.org/10.1128/mbio.01966-22
Millions of people are infected with Toxoplasma gondii, and the available treatment for toxoplasmosis is not ideal. Most of the drugs currently used are only effective for the acute infection, and treatment can trigger serious side effects requiring ...
Host-Microbial InteractionsResearch Article24 August 2022
Mycobacterium tuberculosis Utilizes Host Histamine Receptor H1 to Modulate Reactive Oxygen Species Production and Phagosome Maturation via the p38MAPK-NOX2 Axis
  • Siwei Mo,
  • Jiubiao Guo,
  • Taosheng Ye,
  • Ximeng Zhang,
  • Jiang Zeng,
  • Yuzhong Xu,
  • Bin Peng,
  • Youchao Dai,
  • Wei Xiao,
  • Peize Zhang,
  • Guofang Deng,
  • Dechang Xu,
  • Xiaoru Long,
  • Yi Cai,
  • Xinchun Chen
https://doi.org/10.1128/mbio.02004-22
Once engulfed in macrophage phagosomes, M. tuberculosis adopts various strategies to take advantage of the host environment for its intracellular survival. Histamine is an organic nitrogen-containing compound that mediates a plethora of cellular processes ...
BacteriologyResearch Article31 August 2022
Acetylation of Lactate Dehydrogenase Negatively Regulates the Acidogenicity of Streptococcus mutans
  • Qizhao Ma,
  • Yangyang Pan,
  • Yang Chen,
  • Shuxing Yu,
  • Jun Huang,
  • Yaqi Liu,
  • Tao Gong,
  • Qiong Zhang,
  • Qun Sun,
  • Jing Zou,
  • Yuqing Li
https://doi.org/10.1128/mbio.02013-22
Lysine acetylation, a dynamic regulatory posttranslational modification, remains poorly characterized in bacteria. Hundreds of proteins have been identified to be acetylated in bacteria, with advances made in acetylome analyses.
BacteriologyResearch Article16 August 2022
Evidence for a Widespread Third System for Bacterial Polysaccharide Export across the Outer Membrane Comprising a Composite OPX/β-Barrel Translocon
  • Johannes Schwabe,
  • María Pérez-Burgos,
  • Marco Herfurth,
  • Timo Glatter,
  • Lotte Søgaard-Andersen
https://doi.org/10.1128/mbio.02032-22
Bacteria secrete a wide variety of polysaccharides that have critical functions in, e.g., fitness, surface colonization, and biofilm formation and in beneficial and pathogenic human-, animal-, and plant-microbe interactions. In Gram-negative bacteria, ...
Clinical MicrobiologyResearch Article22 August 2022
Microbial Analysis of Umbilical Cord Blood Reveals Novel Pathogens Associated with Stillbirth and Early Preterm Birth
  • Emilie L. Vander Haar,
  • Guojun Wu,
  • Cynthia Gyamfi-Bannerman,
  • Charlene Thomas,
  • Ronald J. Wapner,
  • Uma M. Reddy,
  • Liping Zhao,
  • Robert M. Silver,
  • Robert L. Goldenberg,
  • Yiping W. Han
https://doi.org/10.1128/mbio.02036-22
Stillbirth accounts for half of all perinatal mortality, but the underlying cause of a substantial portion of all cases remains elusive. We examined the umbilical cord blood microbiome in stillbirths (n = 60) and live births (n = 176) and discovered that ...
VirologyResearch Article16 August 2022
Herpes Simplex Virus 1 Entry Glycoproteins Form Complexes before and during Membrane Fusion
  • Zemplen Pataki,
  • Andrea Rebolledo Viveros,
  • Ekaterina E. Heldwein
https://doi.org/10.1128/mbio.02039-22
Herpes simplex virus 1 (HSV-1) infects the majority of humans for life and can cause diseases ranging from painful sores to deadly brain inflammation. No vaccines or curative treatments currently exist.
VirologyResearch Article24 August 2022
Multiple Introductions of SARS-CoV-2 Alpha and Delta Variants into White-Tailed Deer in Pennsylvania
  • Andrew D. Marques,
  • Scott Sherrill-Mix,
  • John K. Everett,
  • Hriju Adhikari,
  • Shantan Reddy,
  • Julie C. Ellis,
  • Haley Zeliff,
  • Sabrina S. Greening,
  • Carolyn C. Cannuscio,
  • Katherine M. Strelau,
  • Ronald G. Collman,
  • Brendan J. Kelly,
  • Kyle G. Rodino,
  • Frederic D. Bushman,
  • Roderick B. Gagne,
  • Eman Anis
https://doi.org/10.1128/mbio.02101-22
Coronaviruses have been documented to replicate in numerous species of vertebrates, and multiple spillovers of coronaviruses from animals into humans have founded human epidemics. The COVID-19 epidemic likely derived from a spillover of SARS-CoV-2 from ...
Editor's PickMolecular and Cellular BiologyResearch Article07 September 2022
Import and Export of Mannosylerythritol Lipids by Ustilago maydis
  • Fabienne Becker,
  • Uwe Linne,
  • Xiulan Xie,
  • Anna-Lena Hemer,
  • Michael Bölker,
  • Johannes Freitag,
  • Björn Sandrock
https://doi.org/10.1128/mbio.02123-22
Fungi produce and secrete various secondary metabolites that can act as weapons against competitors, help in accessing nutrients, or assist in development and communication. One group of secondary metabolites are surface-active glycolipids, which have ...
Structural BiologyResearch Article29 August 2022
Dual Targeting Factors Are Required for LXG Toxin Export by the Bacterial Type VIIb Secretion System
  • Timothy A. Klein,
  • Dirk W. Grebenc,
  • Prakhar Y. Shah,
  • Owen D. McArthur,
  • Brandon H. Dickson,
  • Michael G. Surette,
  • Youngchang Kim,
  • John C. Whitney
https://doi.org/10.1128/mbio.02137-22
Type VIIb secretion systems (T7SSb) are protein secretion machines used by an array of Gram-positive bacterial genera, including Staphylococcus, Streptococcus, Bacillus, and Enterococcus. These bacteria use the T7SSb to facilitate interbacterial killing ...
ImmunologyResearch Article23 August 2022
Genomic Epidemiology and Serology Associated with a SARS-CoV-2 R.1 Variant Outbreak in New Jersey
  • Barun Mathema,
  • Liang Chen,
  • Pengfei Wang,
  • Marcus H. Cunningham,
  • Jose R. Mediavilla,
  • Kar Fai Chow,
  • Yang Luo,
  • Yanan Zhao,
  • Kaelea Composto,
  • Jerry Zuckerman,
  • Michael C. Zody,
  • Nancy Wilson,
  • Annie Lee,
  • Dayna M. Oschwald,
  • Lihong Liu,
  • Sho Iketani,
  • Soren Germer,
  • Samantha Fennessey,
  • Maple Wang,
  • Yael Kramer,
  • Patricia Toole,
  • Tom Maniatis,
  • David D. Ho,
  • David S. Perlin,
  • Barry N. Kreiswirth
https://doi.org/10.1128/mbio.02141-22
The neutralizing capacities of monoclonal antibodies used to treat COVID-19 and of those recovered from previously infected and vaccinated individuals against SARS-CoV-2 variants of concern (VOCs) remain important questions. We report on a nosocomial ...
BacteriologyResearch Article21 September 2022
Pseudomonas aeruginosa Production of Hydrogen Cyanide Leads to Airborne Control of Staphylococcus aureus Growth in Biofilm and In Vivo Lung Environments
  • Sylvie Létoffé,
  • Yongzheng Wu,
  • Sophie E. Darch,
  • Christophe Beloin,
  • Marvin Whiteley,
  • Lhousseine Touqui,
  • Jean-Marc Ghigo
https://doi.org/10.1128/mbio.02154-22
Airborne volatile compounds produced by bacteria are often only considered attractive or repulsive scents, but they also directly contribute to bacterial physiology. Here, we showed that volatile hydrogen cyanide (HCN) released by a wide range of ...
Host-Microbial InteractionsResearch Article12 September 2022
Siderophore Immunization Restricted Colonization of Adherent-Invasive Escherichia coli and Ameliorated Experimental Colitis
  • Romana R. Gerner,
  • Suzana Hossain,
  • Artur Sargun,
  • Kareem Siada,
  • Grant J. Norton,
  • Tengfei Zheng,
  • Wilma Neumann,
  • Sean-Paul Nuccio,
  • Elizabeth M. Nolan,
  • Manuela Raffatellu
https://doi.org/10.1128/mbio.02184-22
Adherent-invasive Escherichia coli (AIEC) is abnormally prevalent in patients with ileal Crohn’s disease and exacerbates intestinal inflammation, but treatment strategies that selectively target AIEC are unavailable. Iron is an essential micronutrient for ...
Microbial PathogenesisResearch Article20 September 2022
Uptake of the Siderophore Triacetylfusarinine C, but Not Fusarinine C, Is Crucial for Virulence of Aspergillus fumigatus
  • Mario Aguiar,
  • Thomas Orasch,
  • Yana Shadkchan,
  • Patricia Caballero,
  • Joachim Pfister,
  • Luis Enrique Sastré-Velásquez,
  • Fabio Gsaller,
  • Clemens Decristoforo,
  • Nir Osherov,
  • Hubertus Haas
https://doi.org/10.1128/mbio.02192-22
Aspergillus fumigatus is responsible for thousands of cases of invasive fungal disease annually. For iron uptake, A. fumigatus secretes so-called siderophores, which are taken up after the binding of environmental iron.
Host-Microbial InteractionsResearch Article31 August 2022
Postinfection Metabolic Reprogramming of the Murine Trigeminal Ganglion Limits Herpes Simplex Virus-1 Replication
  • Chandrashekhar D. Patil,
  • Rahul K. Suryawanshi,
  • Divya Kapoor,
  • Deepak Shukla
https://doi.org/10.1128/mbio.02194-22
The more severe ocular pathologies associated with HSV-1 infection are significant vision loss, ocular morbidity, and herpetic keratitis. The current clinical landscape lacks curative drugs and vaccines against HSV-1, a heavy burden associated with this ...
Human MicrobiomeResearch Article03 October 2022
The Urethral Microbiota of Men with and without Idiopathic Urethritis
  • Erica L. Plummer,
  • Larissa K. Ratten,
  • Lenka A. Vodstrcil,
  • Gerald L. Murray,
  • Jennifer A. Danielewski,
  • Christopher K. Fairley,
  • Suzanne M. Garland,
  • Eric P. F. Chow,
  • Catriona S. Bradshaw
https://doi.org/10.1128/mbio.02213-22
Nongonococcal urethritis (NGU) is the commonest genital tract syndrome in men and is nearly universally presumptively treated with an antibiotic. Common causes of NGU include Chlamydia trachomatis and Mycoplasma genitalium, but in more than 50% of cases, ...
VirologyResearch Article29 August 2022
Adaptation of HIV-1/HIV-2 Chimeras with Defects in Genome Packaging and Viral Replication
  • Jonathan M. O. Rawson,
  • Olga A. Nikolaitchik,
  • Jennifer A. Yoo,
  • Xayathed Somoulay,
  • Matthew A. Brown,
  • Franck S. Mbuntcha Bogni,
  • Vinay K. Pathak,
  • Ferri Soheilian,
  • Ryan L. Slack,
  • Stefan G. Sarafianos,
  • Wei-Shau Hu
https://doi.org/10.1128/mbio.02220-22
Novel retroviruses can emerge from recombination between distantly related retroviruses. Most notably, HIV-1 originated from zoonotic transmission of a novel recombinant (SIVcpz) into humans.
VirologyResearch Article07 September 2022
Treatment of Sindbis Virus-Infected Neurons with Antibody to E2 Alters Synthesis of Complete and nsP1-Expressing Defective Viral RNAs
  • Jane X. Yeh,
  • Yunfan Fan,
  • Maggie L. Bartlett,
  • Xiaoyan Zhang,
  • Norah Sadowski,
  • Debra A. Hauer,
  • Winston Timp,
  • Diane E. Griffin
https://doi.org/10.1128/mbio.02221-22
Alphaviruses are important causes of viral encephalomyelitis without approved treatments or vaccines. Antibody to the Sindbis virus (SINV) E2 glycoprotein is required for immune-mediated noncytolytic virus clearance from neurons.
Genetics and Molecular BiologyResearch Article26 September 2022
Plasmodium falciparum CRK5 Is Critical for Male Gametogenesis and Infection of the Mosquito
  • Sudhir Kumar,
  • Olivia R. Gargaro,
  • Stefan H. I. Kappe
https://doi.org/10.1128/mbio.02227-22
Gametocytes are parasite sexual stages which differentiate from asexually replicating parasites. These stages are necessary for the completion of sexual phase of the parasite life cycle.
MycologyResearch Article26 September 2022
Niemann-Pick Type C Proteins Are Required for Sterol Transport and Appressorium-Mediated Plant Penetration of Colletotrichum orbiculare
  • Sayo Kodama,
  • Naoki Kajikawa,
  • Fumi Fukada,
  • Yasuyuki Kubo
https://doi.org/10.1128/mbio.02236-22
Fungal morphogenesis requires accurate cell cycle progression. Two-component GTPase-activating protein (GAP) CoBub2-CoBfa1 interacts with downstream GTPase CoTem1 and is required for G1/S progression to establish plant infection in Colletotrichum ...
Genetics and Molecular BiologyResearch Article21 September 2022
Multiple Mechanisms for Copper Uptake by Methylosinus trichosporium OB3b in the Presence of Heterologous Methanobactin
  • Peng Peng,
  • Wenyu Gu,
  • Alan A. DiSpirito,
  • Jeremy D. Semrau
https://doi.org/10.1128/mbio.02239-22
Methanotrophs play a critical role in the global carbon cycle, as well as in future strategies for mitigating climate change through their consumption of methane, a trace atmospheric gas much more potent than carbon dioxide in global warming potential. ...
VirologyResearch Article23 August 2022
Marburg Virus VP30 Is Required for Transcription Initiation at the Glycoprotein Gene
  • Megan R. Edwards,
  • Olivia A. Vogel,
  • Hiroyuki Mori,
  • Robert A. Davey,
  • Christopher F. Basler
https://doi.org/10.1128/mbio.02243-22
Marburg virus and Ebola virus encode VP30 proteins. While the role of VP30 in Ebola virus transcription has been well studied, the role of VP30 in the Marburg virus life cycle is not well understood.
Host-Microbial InteractionsResearch Article19 September 2022
Preexisting Heterogeneity of Inducible Nitric Oxide Synthase Expression Drives Differential Growth of Mycobacterium tuberculosis in Macrophages
  • Ophélie Rutschmann,
  • Chiara Toniolo,
  • John D. McKinney
https://doi.org/10.1128/mbio.02251-22
In this report, we demonstrate that fluctuations in the expression of antimicrobial genes can define how single host cells control bacterial infections. We show that preexisting cell-to-cell variation in the expression of a single gene, that for inducible ...
MycologyResearch Article28 September 2022
The Cryptococcus neoformans Flc1 Homologue Controls Calcium Homeostasis and Confers Fungal Pathogenicity in the Infected Hosts
  • Piotr R. Stempinski,
  • Kristie D. Goughenour,
  • Lukas M. du Plooy,
  • J. Andrew Alspaugh,
  • Michal A. Olszewski,
  • Lukasz Kozubowski
https://doi.org/10.1128/mbio.02253-22
Cryptococcosis is a highly lethal infection with limited drug choices, most of which are highly toxic or complicated by emerging antifungal resistance. There is a great need for new drug targets that are unique to the fungus.
ImmunologyResearch Article30 August 2022
Neuroimmune Responses in a New Experimental Animal Model of Cerebral Aspergillosis
  • Brianne N. Sullivan,
  • Mia A. Baggett,
  • Christa Guillory,
  • MaryJane Jones,
  • Chad Steele
https://doi.org/10.1128/mbio.02254-22
Invasive fungal infections (IFIs) result in significant mortality in immunosuppressed individuals. Of these, invasive pulmonary aspergillosis (IPA), caused by the opportunistic mold Aspergillus fumigatus, is the most lethal.
ParasitologyResearch Article08 September 2022
Plasmodium falciparum Genetic Diversity in Coincident Human and Mosquito Hosts
  • Zena Lapp,
  • Andrew A. Obala,
  • Lucy Abel,
  • David A. Rasmussen,
  • Kelsey M. Sumner,
  • Elizabeth Freedman,
  • Steve M. Taylor,
  • Wendy Prudhomme-O’Meara
https://doi.org/10.1128/mbio.02277-22
Plasmodium falciparum is the deadliest human malaria parasite, and infections consisting of concurrent, multiple strains are common in regions of high endemicity. During transitions within and between the parasite’s mosquito and human hosts, these strains ...
BacteriologyResearch Article27 September 2022
Small Molecule Antibiotics Inhibit Distinct Stages of Bacterial Outer Membrane Protein Assembly
  • Janine H. Peterson,
  • Matthew Thomas Doyle,
  • Harris D. Bernstein
https://doi.org/10.1128/mbio.02286-22
There is a clear need to develop novel broad-spectrum antibiotics to address the global problem of antimicrobial resistance. Multiple compounds that have strong antibacterial activity have recently been described that appear to inhibit the activity of the ...
BacteriologyResearch Article07 September 2022
Surface Anchoring of the Kingella kingae Galactan Is Dependent on the Lipopolysaccharide O-Antigen
  • Nina R. Montoya,
  • Eric A. Porsch,
  • Vanessa L. Muñoz,
  • Artur Muszyński,
  • Jiri Vlach,
  • David K. Hahn,
  • Parastoo Azadi,
  • Matthew Sherman,
  • Hyojik Yang,
  • Courtney E. Chandler,
  • Robert K. Ernst,
  • Joseph W. St. Geme III
https://doi.org/10.1128/mbio.02295-22
Kingella kingae is an emerging pediatric pathogen and produces invasive disease by colonizing the oropharynx, invading the bloodstream, and disseminating to distant sites. This organism produces a uniquely multifunctional exopolysaccharide called galactan ...
BacteriologyResearch Article14 September 2022
A Photoconvertible Reporter System for Bacterial Metabolic Activity Reveals That Staphylococcus aureus Enters a Dormant-Like State to Persist within Macrophages
  • Julia C. Lang,
  • Elena A. Seiß,
  • Adriana Moldovan,
  • Mathias Müsken,
  • Till Sauerwein,
  • Martin Fraunholz,
  • Andreas J. Müller,
  • Oliver Goldmann,
  • Eva Medina
https://doi.org/10.1128/mbio.02316-22
The capacity of Staphylococcus aureus to survive and persist within phagocytic cells has been associated with antibiotic treatment failure and recurrent infections. Here, we investigated the molecular mechanisms leading to S. aureus persistence within ...
Editor's PickVaccinesResearch Article06 September 2022
Vaccination with Live or Heat-Killed Aspergillus fumigatus ΔsglA Conidia Fully Protects Immunocompromised Mice from Invasive Aspergillosis
  • Caroline Mota Fernandes,
  • Tyler G. Normile,
  • Joao Henrique Tadini Marilhano Fabri,
  • Veronica Soares Brauer,
  • Glauber R. de S. Araújo,
  • Susana Frases,
  • Leonardo Nimrichter,
  • Iran Malavazi,
  • Maurizio Del Poeta
https://doi.org/10.1128/mbio.02328-22
Infections by Aspergillus fumigatus occur by the inhalation of environmental fungal spores called conidia. We found that live mutant conidia accumulating glycolipids named sterylglucosides are not able to cause disease when injected into the lung.
Microbial PathogenesisResearch Article14 September 2022
Bacteroides thetaiotaomicron Outer Membrane Vesicles Modulate Virulence of Shigella flexneri
  • Nicholas L. Xerri,
  • Shelley M. Payne
https://doi.org/10.1128/mbio.02360-22
Shigella flexneri is the causative agent of bacillary dysentery in humans. Shigella spp. are one of the leading causes of diarrheal morbidity and mortality, especially among children in low- and middle-income countries. The rise of antimicrobial ...
VaccinesResearch Article20 September 2022
A Nonadjuvanted Whole-Inactivated Pneumococcal Vaccine Induces Multiserotype Opsonophagocytic Responses Mediated by Noncapsule-Specific Antibodies
  • Shannon C. David,
  • Erin B. Brazel,
  • Eve V. Singleton,
  • Vikrant Minhas,
  • Zoe Laan,
  • Catherine Scougall,
  • Austen Y. Chen,
  • Hui Wang,
  • Chloe J. Gates,
  • Kimberley T. McLean,
  • Jeremy S. Brown,
  • Giuseppe Ercoli,
  • Rachel A. Higgins,
  • Paul V. Licciardi,
  • Kim Mulholland,
  • Justin B. Davies,
  • Timothy R. Hirst,
  • James C. Paton,
  • Mohammed Alsharifi
https://doi.org/10.1128/mbio.02367-22
The target pathogen of this study, Streptococcus pneumoniae, kills over 300,000 children <5 years of age every single year, and is the leading cause of pneumonia-associated mortality globally. While the capsular polysaccharide (CPS)-based vaccine ...
BacteriologyResearch Article06 October 2022
Spontaneous Prophage Induction Contributes to the Production of Membrane Vesicles by the Gram-Positive Bacterium Lacticaseibacillus casei BL23
  • David da Silva Barreira,
  • Pierre Lapaquette,
  • Julia Novion Ducassou,
  • Yohann Couté,
  • Jean Guzzo,
  • Aurélie Rieu
https://doi.org/10.1128/mbio.02375-22
The last few decades have demonstrated that membrane vesicles (MVs) produced by microorganisms can have a wide variety of functions. This diversity places MVs at the crossroads of major research topics in current microbiology such as antibiotic resistance,...
Host-Microbial InteractionsResearch Article26 September 2022
PopB-PcrV Interactions Are Essential for Pore Formation in the Pseudomonas aeruginosa Type III Secretion System Translocon
  • Emma Kundracik,
  • Josephine Trichka,
  • José Díaz Aponte,
  • Alicia Roistacher,
  • Arne Rietsch
https://doi.org/10.1128/mbio.02381-22
Type III secretion systems are integral to the pathogenesis of many Gram-negative bacterial pathogens. A hallmark of these secretion systems is that they deliver effector proteins vectorially into the targeted host cell via a translocation pore.
Host-Microbial InteractionsResearch Article20 September 2022
SARS-CoV-2 Diverges from Other Betacoronaviruses in Only Partially Activating the IRE1α/XBP1 Endoplasmic Reticulum Stress Pathway in Human Lung-Derived Cells
  • Long C. Nguyen,
  • David M. Renner,
  • Diane Silva,
  • Dongbo Yang,
  • Nicholas A. Parenti,
  • Kaeri M. Medina,
  • Vlad Nicolaescu,
  • Haley Gula,
  • Nir Drayman,
  • Andrea Valdespino,
  • Adil Mohamed,
  • Christopher Dann,
  • Kristin Wannemo,
  • Lydia Robinson-Mailman,
  • Alan Gonzalez,
  • Letícia Stock,
  • Mengrui Cao,
  • Zeyu Qiao,
  • Raymond E. Moellering,
  • Savas Tay,
  • Glenn Randall,
  • Michael F. Beers,
  • Marsha Rich Rosner,
  • Scott A. Oakes,
  • Susan R. Weiss
https://doi.org/10.1128/mbio.02415-22
SARS-CoV-2 is the third lethal respiratory coronavirus, after MERS-CoV and SARS-CoV, to emerge this century, causing millions of deaths worldwide. Other common coronaviruses such as HCoV-OC43 cause less severe respiratory disease.
Pathogenesis and Host ResponseResearch Article22 September 2022
Comparative Assessment of Severe Acute Respiratory Syndrome Coronavirus 2 Variants in the Ferret Model
  • Joanna A. Pulit-Penaloza,
  • Jessica A. Belser,
  • Xiangjie Sun,
  • Claudia Pappas,
  • Nicole Brock,
  • Troy J. Kieran,
  • Jana M. Ritter,
  • Josilene N. Seixas,
  • Joyce Jones,
  • Poulami Basu Thakur,
  • Elizabeth Pusch,
  • Li Wang,
  • Terrence M. Tumpey,
  • David E. Wentworth,
  • Bin Zhou,
  • Taronna R. Maines
https://doi.org/10.1128/mbio.02421-22
Continued surveillance and risk assessment of emerging SARS-CoV-2 variants are critical for pandemic response and preparedness. As such, in vivo evaluations are indispensable for early detection of variants with enhanced virulence and transmission.
Microbial PathogenesisResearch Article05 October 2022
Diisonitrile Lipopeptides Mediate Resistance to Copper Starvation in Pathogenic Mycobacteria
  • John A. Buglino,
  • Yaprak Ozakman,
  • Yao Xu,
  • Farhan Chowdhury,
  • Derek S. Tan,
  • Michael S. Glickman
https://doi.org/10.1128/mbio.02513-22
Bacterial pathogens and their hosts engage in intense competition for nutrients, including metals. Mycobacterium tuberculosis, the cause of tuberculosis, lives within host macrophages and is subject to diverse stresses, including metal excess and metal ...
VirologyResearch Article05 October 2022
The Integral Membrane Protein ZMPSTE24 Protects Cells from SARS-CoV-2 Spike-Mediated Pseudovirus Infection and Syncytia Formation
  • Khurts Shilagardi,
  • Eric D. Spear,
  • Rachy Abraham,
  • Diane E. Griffin,
  • Susan Michaelis
https://doi.org/10.1128/mbio.02543-22
The COVID-19 pandemic caused by the coronavirus SARS-CoV-2 has underscored the importance of understanding intrinsic cellular components that can be harnessed as the cell’s first line of defense to fight against viral infection. Our paper focuses on one ...
Genetics and Molecular BiologyResearch Article21 September 2022
Top-Down, Knowledge-Based Genetic Reduction of Yeast Central Carbon Metabolism
  • Eline D. Postma,
  • Lucas G. F. Couwenberg,
  • Roderick N. van Roosmalen,
  • Jordi Geelhoed,
  • Philip A. de Groot,
  • Pascale Daran-Lapujade
https://doi.org/10.1128/mbio.02970-21
Fundamental questions regarding the minimal requirements for life have prompted scientists to embark on top-down efforts to reduce microbial genomes to the minimum set of genes and proteins necessary to sustain cell survival and division. While these ...

Author Corrections

Correction31 August 2022
Correction for Merenstein et al., “Signatures of COVID-19 Severity and Immune Response in the Respiratory Tract Microbiome”
  • Carter Merenstein,
  • Guanxiang Liang,
  • Samantha A. Whiteside,
  • Ana G. Cobián-Güemes,
  • Madeline S. Merlino,
  • Louis J. Taylor,
  • Abigail Glascock,
  • Kyle Bittinger,
  • Ceylan Tanes,
  • Jevon Graham-Wooten,
  • Layla A. Khatib,
  • Ayannah S. Fitzgerald,
  • Shantan Reddy,
  • Amy E. Baxter,
  • Josephine R. Giles,
  • Derek A. Oldridge,
  • Nuala J. Meyer,
  • E. John Wherry,
  • John E. McGinniss,
  • Frederic D. Bushman,
  • Ronald G. Collman
https://doi.org/10.1128/mbio.02293-22
Volume 12, no. 4, e01777-21. https://doi.org/10.1128/mBio.01777-21. In Table S5 in the supplemental material, two primer sequences were listed incorrectly. The actual sequence of TTV_3656_Fwd is AGACTCCGAGDTGCYMTTGG, and the actual sequence of TTV_3656_Rev is CTYAAAATGGCBGCYGTGAC.
Correction25 August 2022
Correction for Suerbaum et al., “Identification of Antimotilins, Novel Inhibitors of Helicobacter pylori Flagellar Motility That Inhibit Stomach Colonization in a Mouse Model”
  • Sebastian Suerbaum,
  • Nina Coombs,
  • Lubna Patel,
  • Dimitri Pscheniza,
  • Katharina Rox,
  • Christine Falk,
  • Achim D. Gruber,
  • Olivia Kershaw,
  • Patrick Chhatwal,
  • Mark Brönstrup,
  • Ursula Bilitewski,
  • Christine Josenhans
https://doi.org/10.1128/mbio.02301-22
Volume 13, no. 2, e03755-21, https://doi.org/10.1128/mBio.03755-21. One of the sources of funding for this study was not listed in the Acknowledgments section. The corrected Acknowledgments section should appear as shown below.
Correction03 October 2022
Correction for Zhao et al., “Gasdermin D Inhibits Coronavirus Infection by Promoting the Noncanonical Secretion of Beta Interferon”
  • Liyuan Zhao,
  • Liang Li,
  • Mei Xue,
  • Xiang Liu,
  • Chengfan Jiang,
  • Wenzhe Wang,
  • Lijie Tang,
  • Li Feng,
  • Pinghuang Liu
https://doi.org/10.1128/mbio.02586-22
Volume 13, no. 1, e03600-21, 2022, https://doi.org/10.1128/mbio.03600-21. In Fig. 2I, there are partial duplications in the N-GSDMD_1-242 and N-GSDMD_1-252 panels and the N-GSDMD_1-252 and N-GSDMD_1-262 panels.

Author Reply

Antimicrobial ChemotherapyLetter22 August 2022
Reply to Luger, “Why Is It So Hard to Find Persistent Borreliella burgdorferi?”
  • Felipe C. Cabello,
  • Monica E. Embers,
  • Stuart A. Newman,
  • Henry P. Godfrey
https://doi.org/10.1128/mbio.02169-22
We thank Dr. Steven W. Luger for his interest in our review and his clinically related comments. We have responded to them below.
BacteriologyLetter15 September 2022
Reply to Oren et al., “New Phylum Names Harmonize Prokaryotic Nomenclature”
  • Gaurav Sharma,
  • Salim T. Islam,
  • Praveen Rahi,
  • Mark W. Silby,
  • Donato Giovannelli,
  • Paula V. Welander,
  • Monika Ehling-Schulz,
  • Vasvi Chaudhry,
  • Evelyn Molloy,
  • Christian Hertweck,
  • Sunil Mundra,
  • Vipin Chandra Kalia,
  • Rup Lal,
  • Yogendra Singh,
  • Edward Ruby,
  • Christoph Weigel,
  • Roberto Kolter
https://doi.org/10.1128/mbio.02323-22
We thank Oren et al., 2022 (1) for providing the International Committee on Systematics of Prokaryotes (ICSP) Executive Board's response to the recent opinion piece “Harmonizing Prokaryotic Nomenclature: Fixing the Fuss over Phylum Name Flipping” (2). Herein, we (a group of concerned microbiologists) offer several additional comments on the naming of prokaryotic phyla. Our concerns arise from the perception that the ICSP Executive Board is not directly addressing the plight of microbiologists around the world concerning the long-lasting confusion that the proposed phylum name changes will create throughout the scientific literature. Instead of selectively pointing out certain ICSP rules, we would have appreciated opening a door for a more inclusive discussion about the process of naming phyla. As previously stated (2), we are in favor of the International Code of Nomenclature of Prokaryotes (ICNP) rules for the recognition of the rank of “phylum” and the usage of “-ota” as the suffix for naming phyla. However, we feel that the drastic name changes of six phyla at the prefix level will create significant dissonance and confusion between the old and new names when considering past and future publications.
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Featured image: A vacuole of intracellular Toxoplasma gondii parasites, expanded by ultrastructure expansion and labeled with anti-acetyl tubulin, observed by confocal microscopy. [See related article: Tomasina et al., mBio 13(5):e01859-22, 2022.] © Tomasina et al. This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.

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