Gα and Gβ Proteins Regulate the Cyclic AMP Pathway That Is Required for Development and Pathogenicity of the Phytopathogen Mycosphaerella graminicola
ABSTRACT
We identified and functionally characterized genes encoding three Gα proteins and one Gβ protein in the dimorphic fungal wheat pathogen Mycosphaerella graminicola, which we designated MgGpa1, MgGpa2, MgGpa3, and MgGpb1, respectively. Sequence comparisons and phylogenetic analyses showed that MgGPA1 and MgGPA3 are most related to the mammalian Gαi and Gαs families, respectively, whereas MgGPA2 is not related to either of these families. On potato dextrose agar (PDA) and in yeast glucose broth (YGB), MgGpa1 mutants produced significantly longer spores than those of the wild type (WT), and these developed into unique fluffy mycelia in the latter medium, indicating that this gene negatively controls filamentation. MgGpa3 mutants showed more pronounced yeast-like growth accompanied with hampered filamentation and secreted a dark-brown pigment into YGB. Germ tubes emerging from spores of MgGpb1 mutants were wavy on water agar and showed a nested type of growth on PDA that was due to hampered filamentation, numerous cell fusions, and increased anastomosis. Intracellular cyclic AMP (cAMP) levels of MgGpb1 and MgGpa3 mutants were decreased, indicating that both genes positively regulate the cAMP pathway, which was confirmed because the WT phenotype was restored by adding cAMP to these mutant cultures. The cAMP levels in MgGpa1 mutants and the WT were not significantly different, suggesting that this gene might be dispensable for cAMP regulation. In planta assays showed that mutants of MgGpa1, MgGpa3, and MgGpb1 are strongly reduced in pathogenicity. We concluded that the heterotrimeric G proteins encoded by MgGpa3 and MgGpb1 regulate the cAMP pathway that is required for development and pathogenicity in M. graminicola.
Signal transduction pathways are important for sensing and responding to different environmental stimuli in both lower and higher eukaryotes. The highly conserved heterotrimeric guanine nucleotide-binding proteins (G proteins) belong to a family of regulatory proteins that are crucial for the transduction of signals, which are perceived by a distinct family of cell surface receptors (4). Heterotrimeric G proteins contain three subunits (α, β, and γ) that are linked in the inactive state. Activation of a Gα subunit by a transmembrane receptor leads to exchange of bound GDP with GTP on the Gα subunit, resulting in dissociation of the Gα and the Gβγ dimeric subunits, which can now interact with downstream effectors that subsequently generate changes in cellular responses (for a review, see reference 10).
Filamentous fungi have one Gβ- and usually three Gα-encoding genes that belong to three major groups. Encoded proteins in groups I and III are related to the mammalian Gαi and Gαs families, respectively, but group II fungal Gα proteins have no mammalian counterpart (1, 4, 14, 22, 33, 53). Interestingly, the corn smut fungus Ustilago maydis contains a unique fourth Gα-encoding gene, and Saccharomyces cerevisiae contains only two Gα proteins (10, 57). Irrespective of the observed numerical variation, Gα proteins regulate a variety of cellular and developmental responses (4). For plant-pathogenic fungi, Gβ-encoding genes have been characterized functionally (9, 14, 22, 27, 31, 48, 52). Apart from the fact that individual Gα-encoding genes and the Gβ-encoding gene have been demonstrated to regulate growth, reproduction, and virulence, comparative functional characterization of all Gα-encoding genes has been reported only for a few plant-pathogenic fungi, including Magnaporthe grisea, Cryphonectria parasitica, and U. maydis (5, 41, 57).
Mycosphaerella graminicola (anamorph Septoria tritici) causes septoria tritici blotch disease in bread and durum wheat in areas with high rainfall during the growing season, particularly in Western Europe, where it is considered to be the most important wheat disease (30). It is a ubiquitous phytopathogen with a lifestyle completely different from that of the aforementioned plant-pathogenic fungi. It is a dimorphic pathogen, and therefore the transition from a yeast-like to a filamentous form is important for initiation of infection (45). M. graminicola does not form appressoria but penetrates the leaves through stomata without forming specific infection structures. Furthermore, as a hemibiotroph, it has a biotrophic phase of about 10 days that is followed by a rapid switch to necrotrophy. The necrotic foliar lesions bear anamorphic and teleomorphic fructifications. M. graminicola is the model fungus for the Mycosphaerellaceae and even for the order Dothideales, an extremely large and diverse class of fungi with over 1,000 named species, including major plant pathogens such as the banana leaf streak fungus Mycosphaerella fijiensis (12, 21). Large expressed sequence tag (EST) libraries and the recently released genome sequence have been instrumental for the identification and characterization of genes involved in the development and pathogenicity of M. graminicola (http://genome.jgi-psf.org/Mycgr3/Mycgr3.home.html ). Recently, we reported that genes encoding mitogen-activated protein kinases (MAPKs) (MgFus3, MgSlt2, and MgHog1) and the catalytic (MgTpk2) and regulatory (MgBcy1) subunits of protein kinase A (PKA) are essential pathogenicity factors and regulate specific steps during the infection process (8, 43-45). To extend our knowledge about the role of G proteins in the development and pathogenicity of M. graminicola, we functionally analyzed three Gα-encoding genes and one Gβ-encoding gene of M. graminicola, which we designated MgGpa1, MgGpa2, MgGpa3, and MgGpb1, respectively. Our results show the requirement of MgGpa1, MgGpa3, and MgGpb1 for pathogenicity, whereas the latter also negatively regulates cell fusion and anastomosis. Among the G protein-encoding genes characterized in this study, MgGpa3 and MgGpb1 positively regulate the cyclic AMP (cAMP) pathway. MgGpa1 seems to be dispensable for cAMP regulation, whereas MgGpa2 appears to be redundant, for none of the assays rendered altered phenotypes. Our results open new perspectives for studying the regulatory machinery of the cAMP pathway in M. graminicola and other plant-pathogenic fungi.
MATERIALS AND METHODS
Fungal strains and culture conditions.
DNA manipulations and analysis.
Standard protocols were used for DNA manipulation (58). Plasmid DNA was isolated using a QIAprep Spin miniprep kit (Qiagen, Hilden, Germany). Genomic DNA of M. graminicola was extracted using a Puregene DNA isolation kit (Gentra Systems Inc., Minneapolis, MN). A DYEnamic ET dye terminator cycle sequencing kit (Amersham Biosciences, Roosendaal, The Netherlands) was used to sequence DNA on an ABI Prism 3100 capillary automated sequencer according to recommended protocols. Phylogenetic tree generation, DNA and protein sequence alignment, editing, and analysis were performed using MEGALIGN and DNA Star software (Madison, WI). Homology searches were performed with the BLAST program (2).
Construction of plasmid vectors.
By in silico analyses of EST databases of M. graminicola IPO323 (34), we identified full-length cDNA clones of MgGpa1, MgGpa2, MgGpa3, and MgGpb1. We used the GPS-Mutagenesis system (New England Biolabs, Leusden, The Netherlands) to generate disruption constructs, using a homemade customized donor construct, pGPS3HygKan, that was described previously (44). After transposition, the transposition mixture was cloned into Escherichia coli strain DH10B (Invitrogen, Groningen, The Netherlands) by electroporation, using the recommended protocol of the suppliers, and subsequently plated onto LB containing gentamicin and kanamycin to select for colonies with transposon insertions in the target constructs, which were confirmed by multiplex colony PCR as described previously (44). The exact position of the transposon insertion was determined by sequencing from the right and left borders of the transposon, using primer N and primer S (Table 1), and by comparison with nondisrupted sequences. We selected constructs with transposon insertions in the central part of the WT genes, and these were designated pCGN1589ΔMgGpa1, pCGN1589ΔMgGpa2, pCGN1589ΔMgGpa3, and pCGN1589ΔMgGpb1, respectively (Fig. 1A).
Fungal transformation.
The disruption constructs pCGN1589ΔMgGpa1, pCGN1589ΔMgGpa2, pCGN1589ΔMgGpa3, and pCGN1589ΔMgGpb1 were cloned into Agrobacterium tumefaciens strain LBA1100 by electroporation. A. tumefaciens-mediated transformation was performed according to a standard protocol (44, 72). Homologous recombinants were identified by PCR screening (Fig. 1B), using primers that were designed upstream and downstream of the transposon insertions together with either primer S or primer N, located in the left or right border of the transposon, respectively (Table 1). Eventually, we selected two mutant strains and one ectopic transformant for each gene for phenotypic analyses (Table 1). To ensure the independence of the transformants, we performed transformation experiments over time. In cases where transformants were generated in the same experiment, they were collected mostly from different plates (at least four plates for each transformation) to prevent the selection of clones. Moreover, in contrast to polyethylene glycol transformation, A. tumefaciens-mediated transformation precludes the selection of clones, as cocultivation of the fungus and Agrobacterium is done on solid nitrocellulose filters, and hence each colony represents an independent transformation event.
Phenotyping.
We phenotyped all disruptant strains along with ectopic transformants and the WT as control strains.
In vitro assays were performed on agar plates to study colony development by spotting approximately 1 μl of spore suspension (107 spores ml−1) on potato dextrose agar (PDA), which was subsequently cultured for 10 days at 20°C under continuous light, with and without 13 mM exogenous cAMP, or at 28°C in darkness, as well as in liquid yeast glucose broth (YGB) at 20°C under daylight settings. Spore length measurements were performed on 100 randomly selected spores per strain from YGB cultures.
Histological analyses were performed on water agar (WA) and PDA, and the effect of exogenous cAMP (15 mM) on the early development of the strains was examined. Samples were monitored every 24 h until 80 h after initiation of the experiment, using a Zeiss Axioskop microscope (Carl Zeiss, Jena, Germany) equipped with a Carl Zeiss AxioCam 6.08.0 digital camera connected to an IBM computer. Pictures were saved with Axio Vision 4.1 software (Carl Zeiss, Jena, Germany), and composed pictures were prepared with Adobe Photoshop 7.0.
cAMP measurements.
Quantification of intracellular cAMP was performed on 12 isolates (Table 1), including the WT, according to the protocol suggested by the manufacturer (Amersham Life Science Inc., Arlington Heights, IL). Spores were collected and lyophilized after being cultured for 5 days in YGB. Lyophilized spores (100 mg) were ground to a fine powder in a 2-ml Eppendorf tube by use of a bead beater (Ribolyser; Hybaid, Heidelberg, Germany). Powdered mycelia were resuspended in distilled water, and proteins were precipitated by adding 700 μl of 6% tricarboxylic acid (TCA). After centrifugation, the supernatant was extracted five times with water-saturated ether to remove the TCA and subsequently lyophilized. The lyophilized sample was dissolved in phosphate buffer (10 mM, pH 7.4) and used for the cAMP assay. Before protein precipitation with TCA, two replicate samples of 100 μl of the supernatant were taken for protein quantification using a Coomassie Plus protein assay reagent kit (Pierce Biotechnology, Inc.), with bovine serum albumin as a reference. Before cAMP quantification, the pH was measured, and if necessary, 50% KHCO3 was added until a pH of 6 to 8 was obtained. In total, three biological replicates of each strain were assayed in duplicate by a cAMP (3H) assay system following the manufacturer's instructions (Amersham Life Science Inc., Arlington Heights, IL). After quantification, the cAMP values were normalized to the protein level for each strain (pmol cAMP/mg total protein).
Statistical analyses.
cAMP (pmol cAMP/mg total protein) and spore length (μm) data were natural log transformed and further analyzed by analysis of variance followed by pairwise comparisons among treatment means (based on t tests with a P value of 0.05) to test for significant differences between experiments, using the statistical software package Genstat (54) extended with Biometris Procedure library applications (20). The analyses did not show significant differences between control strains (WT and ectopic transformants) and between the independent mutants of the same gene, which allowed us to lump these data into groups for final analyses (controls and MgGpa1, MgGpa3, and MgGpb1 mutants).
Nucleotide sequence accession numbers.
Sequence data from this article were deposited with the EMBL/GenBank data libraries under accession numbers DQ458049, DQ458050, DQ458051, and DQ458052, for MgGpa1, MgGpa2, MgGpa3, and MgGpb1, respectively.
RESULTS
Sequence comparison and phylogenetic analyses of MgGpa1, MgGpa2, MgGpa3, and MgGpb1.
Screening of a large EST resource comprising 27,007 M. graminicola ESTs (34) enabled us to identify three Gα-encoding genes and one Gβ-encoding gene. Assembly of individual reads of these ESTs combined with sequencing of full-length clones resulted in identification of the genes MgGpa1, MgGpa2, MgGpa3, and MgGpb1, with 1,059-, 1,065-, 1,068-, and 1,050-bp open reading frames encoding proteins consisting of 353, 355, 356, and 350 amino acids, respectively. Screening of the recently released genome sequence of M. graminicola IPO323 (U.S. Department of Energy Joint Genome Institute [http://genome.jgi-psf.org/Mycgr3/Mycgr3.home.html ]) revealed no additional Gα- and Gβ-encoding genes, but we identified an expected putative Gγ protein-encoding gene, showing 60% identity with the Gγ subunit of Aspergillus fumigatus (GenBank accession number XP_750270), which was not present in the EST database (34) and hence was not considered in this project.
Comparison of proteins encoded by these genes with orthologs in M. grisea showed that MgGPA1, MgGPA2, MgGPA3, and MgGPB1 are the orthologs of MAGB, MAGC, MAGA, and MGB1, with 89, 76, 87, and 88% similarity, respectively. Analyses of the genomic DNA sequences and the EST contigs of these genes revealed that MgGpa1, MgGpa2, MgGpa3, and MgGpb1 contain one, four, five, and four introns, respectively. Multiple sequence alignments of the fungal Gα protein sequences resulted in a phylogenetic tree with three major groups within the superfamily of the Gα proteins (Fig. 2A). All Gα proteins (MgGPA1, MgGPA2, and MgGPA3) contain the characteristic P-loop motif, which is one of the ATP/GTP binding regions (G/AXXXXGKT/S), and a G motif that stabilizes the guanine ring (24, 55). MgGPA1 belongs to major group I and contains the consensus myristoylation site, indicated as MGX (an uncharged residue), XX (small uncharged residues), S at the N terminus, and a consensus CXXX sequence at the C terminus, which is the fingerprint of the pertussis toxin labeling site (17, 60). Multiple sequence alignments showed that MgGPA2 has no mammalian counterpart and belongs to group II, which is a distinct group lacking the consensus myristoylation site (49). MgGPA3 belongs to major group III, which has only the consensus myristoylation sequence, which is characteristic for adenylyl cyclase-stimulating G proteins (Gαs).
Protein comparisons via an NCBI conserved domain search showed that MgGPB1 contains seven WD40 domains, which are characteristic of a family of ancient regulatory proteins (51). WD40 repeats in M. graminicola generally contain a C-terminal GH dipeptide, an inter-WD40 region of 13 to 29 amino acids, and an N-terminal WD dipeptide, representing the WD40 signature. However, there is variation in either one or both GH/WD dipeptides. The second WD40 repeat (residues 104 to 134) in MgGPB1 is the most variable domain among fungi and contains LR/YN at the C/N termini in M. graminicola instead of GH/WD. The other WD40 domains are highly conserved in one or both of the C/N termini.
Transposon-mediated vector construction and targeted gene disruption.
The disruption constructs for MgGpa1, MgGpa2, MgGpa3, and MgGpb1 were generated using an in vitro transposition system as described previously (44). PCR screening and sequencing enabled the selection of constructs with transposon insertions downstream of the start codon in the open reading frames of MgGpa1, MgGpa2, MgGpa3, and MgGpb1, at 666, 772, 393, and 586 bp, respectively (Fig. 1A). We used these constructs to disrupt MgGpa1, MgGpa2, MgGpa3, and MgGpb1 in the WT through A. tumefaciens-mediated transformation (44, 72). After transformation, homologous recombinants were identified by PCR screening (Fig. 1B). Among 28, 25, 92, and 47 hygromycin-resistant transformants for MgGpa1, MgGpa2, MgGpa3, and MgGpb1, we identified 3, 14, 3, and 2 disruptant strains, respectively, and 12 transformants were selected for further studies (Table 1).
Phenotypic characteristics.
The MgGpa1, MgGpa3, and MgGpb1 mutants showed altered phenotypes on PDA at 20°C under continuous light (Fig. 3A) and darkness (not shown), whereas the phenotypes of MgGpa2 mutants were identical to those of the controls under all conditions tested (not shown). The colonies of the WT and ectopic transformants started to melanize after incubation for 4 to 5 days and were completely melanized and covered with a thin layer of white aerial mycelium 10 days after incubation, whereas the colonies of MgGpa1 and MgGpb1 mutants were nonmelanized and showed fimbriated borders (Fig. 3A). Spore size measurements showed that the MgGpa1 disruptants produced significantly longer spores in YGB than those of the WT and ectopic controls (Table 2; Fig. 4) (WT and ectopic control spores were 32.2 μm long, whereas transformant spores were ≥62.8 μm long; P < 0.05). The growth rate of the MgGpb1 mutant was significantly reduced (∼25% colony area, with fimbriated borders 56% of those of the WT after 10 days), whereas the MgGpa3 mutant showed only a slight growth reduction and delayed melanization. In addition, the MgGpa3 mutant exhibited an extensive yeast-like growth in the center of the colonies, which was characterized by an intensive microconidiation and a concatenation of budding cells (Fig. 3A). Interestingly, such a tendency to yeast-like growth and reduced filamentation was also observed in the MgTpk2 mutant, in which the catalytic subunit of PKA was disrupted (43). At 28°C, the growth patterns and phenotypes of the WT and ectopic transformant controls and the mutants were nearly similar and were characterized by a fluffy white aerial mycelium that covered the colony (not shown). At temperatures of >29°C, all colonies stopped growing, including the WT and ectopic transformant controls (not shown). In YGB, controls and all mutants merely produced yeast-like cells, except for the MgGpa1 mutants, which produced fluffy mycelia (Fig. 3B). MgGpa3 mutants secreted a dark brown pigment into YGB (Fig. 3C), similar to our previous observation with the MgTpk2 mutant (43).
Complementation of mutants with cAMP.
To test whether the G proteins are involved in the cAMP-dependent signaling pathway, we supplemented PDA with exogenous cAMP (Fig. 3A). This had pleiotropic effects on the WT and all mutants. In the WT, cAMP complementation had multiple effects on appearance, filamentation, and growth rate, resulting in a smaller colony (∼36% of the WT colony area) that was covered with white aerial mycelium. Exogenous cAMP restored the phenotypes of all mutants to the above-mentioned WT phenotype (Fig. 3A).
Microscopic analyses.
We monitored germination, early colony formation, and conidiation in all mutants in the absence and presence of cAMP to further explore the aforementioned phenotype restoration by exogenous cAMP (Table 2; Fig. 4 and 5). On WA, the controls initially germinated from both apical cells of the spores, producing relatively long primary germ tubes within 12 h. Subsequently, secondary germ tubes from the same or other cells of the spore were formed. Within 48 h, tertiary hyphal filaments developed from the primary and secondary germ tubes. We did not observe significantly altered initial germination patterns for the MgGpa1, MgGpa2, and MgGpa3 mutants. However, at later stages, MgGpa1 mutants produced longer germ tubes than the WT or ectopic transformants did. MgGpb1 mutants grew significantly slower, with wavy germ tubes, and secondary and tertiary filaments as well as some hyphae showed anastomosis after growth for 80 h (data not shown).
On PDA, the WT and ectopic controls formed shorter germ tubes that often showed differentiated budding cells on apical and intermediate cells of the spore as well as on secondary filaments within 24 h. These extended into filamentous colonies at 48 h, and these showed abundant microconidiation in the colony center after 80 h. Compared to the WT, the MgGpa1 mutants showed longer germ tubes and increased filamentation, with altered polarized growth, reduced branching (>24 h), and long uniform spores (>48 h) (Table 2; Fig. 4). MgGpa3 disruptant spores produced short germ tubes without secondary or tertiary filaments (>24 h). Colony formation was associated with extensive budding, resulting in dense colonies of typical uniform spores (>48 h) (Table 2; Fig. 5). The MgGpb1 mutant produced germ tubes that fused within 48 h and subsequently produced an extremely dense biomass between 48 and 80 h, which resulted from seemingly uncontrolled continuous anastomosis (Table 2; Fig. 5 and 6). This process greatly suppressed filamentous growth and significantly reduced microconidiation. The addition of cAMP to the medium reduced the colony size of the WT and MgGpa1 mutants but hardly affected the growth characteristics of the latter. For the MgGpa3 and MgGpb1 mutants, exogenous cAMP resulted in the formation of colonies that were very similar to the WT, suggesting that these genes may function in the cAMP pathway.
Intracellular cAMP measurements.
In order to strengthen the macroscopic (Fig. 3) and microscopic (Fig. 5 and 6) observations in the presence and absence of exogenous cAMP, we performed intracellular cAMP measurements on all mutants. The MgGpa3 and MgGpb1 mutants showed significantly reduced levels of cAMP. In contrast, internal cAMP measurement data for the MgGpa1 mutant and the WT were not significantly different (Table 2).
In planta characterization of mutants.
Ten-day-old seedlings of wheat cv. Obelisk inoculated with the WT or ectopic transformants showed chlorotic areas at 8 dpi that continued to expand starting from the leaf tips, which became necrotic at 12 dpi and eventually merged into large necrotic lesions containing numerous pycnidia between 16 and 18 dpi. Plants inoculated with MgGpa1, MgGpa3, or MgGpb1 mutants showed delayed symptom development, with only limited chlorotic areas, starting from the leaf tips, between 12 and 15 dpi. These chlorotic areas sometimes became necrotic but never contained pycnidia, the typical symptom of M. graminicola infection. On the leaf tips of water-treated controls, some chlorosis or necrosis was also observed, most likely as a result of senescence. An overview of disease symptoms caused by the WT and various disruptants and ectopic mutants and a summary of phenotypes of mutants of Gα- and Gβ-encoding genes in other filamentous plant-pathogenic fungi are shown in Fig. 7 and Table 3.
DISCUSSION
The availability of a large EST library and verification with the genome sequence resulted in the identification and isolation of the Gα-encoding genes and the Gβ-encoding gene in M. graminicola. Gene replacement studies revealed that these G proteins are involved in the development and pathogenicity of this fungus. With the exception of MgGpa2, all genes are required for pathogenicity and play an important role in regulation of the dimorphic switch between filamentous and yeast-like growth, microconidiation, or melanization. Phylogenetic analyses indicated that the Gα-encoding genes belong to three different groups. The mammalian orthologs of MgGPA1 and MgGPA3 are Gαi and Gαs, respectively, whereas MgGPA2 has no close relative in mammals. Activation of the Gαi protein in mammals results mostly in inhibition of adenylyl cyclase and hence increases intracellular cAMP levels (5, 6). However, in fungi, the orthologous genes encoding Gαi proteins vary substantially in their regulation of intracellular cAMP levels. For instance, the Cpg-1 gene in C. parasitica negatively regulates the cAMP level, as disruption increased intracellular cAMP levels in this fungus (5). In contrast, the orthologs Gna1 in Neurospora crassa, Fga1 in Fusarium oxysporum, and MagB in M. grisea positively regulate the cAMP pathway, as disruption of these genes decreased intracellular cAMP levels (26, 28). In M. grisea, exogenous cAMP restored appressorium formation and pathogenicity of the MagB mutant (41). In the budding yeast, Gpa1 regulates the MAPK pathway and does not affect the cAMP pathway (3, 59).
Our data show that MgGpa1 is a negative regulator of filamentation, as mutants had significantly longer spores than the WT did. Moreover, phenotypes in YGB and on PDA were characterized by increased filamentous growth, and those on WA were characterized by longer germ tubes. However, the addition of exogenous cAMP hardly affected the phenotypes 80 h and 10 days after initiation of the experiments. Moreover, intracellular cAMP levels in MgGpa1 mutants and the WT were not significantly different. This suggests that MgGpa1 might be dispensable for cAMP regulation, similar to reports for yeast (3, 59). Alternatively, equal intracellular cAMP levels in both the MgGpa1 mutant and the WT strain could also be due to growth characteristics that may influence cAMP extraction efficiencies. For instance, all mutants and controls showed blastic conidiogenesis in YGB, except for MgGpa1 mutants, which showed highly increased filamentous growth. It is possible that such differences in growth pattern affected the intracellular cAMP measurements in the MgGpa1 mutants. In summary, our data suggest that MgGPA1 is functionally different from mammalian inhibitory Gαi proteins and that fungal Gαi-like proteins comprise three subgroups that can either negatively or positively regulate adenylyl cyclase activity or, similar to what has been observed in yeast, do not interfere with the cAMP pathway. This also shows that high protein sequence identities do not necessarily imply similar biological functions.
Gα proteins that belong to group III show high protein sequence identity to stimulatory Gα proteins in N. crassa, C. parasitica, S. cerevisiae, and Schizosaccharomyces pombe. Unlike the Gαi-like proteins, they all positively regulate adenylyl cyclase and are functionally related to mammalian Gαs (16, 25, 33, 39, 42, 50). Significantly reduced intracellular cAMP levels in the MgGpa3 and MgGpb1 mutants confirmed our observation that the addition of exogenous cAMP to these mutants restored their phenotypes to that of the WT and hence suggest that MgGpa3 and MgGpb1 positively regulate adenylyl cyclase activity in M. graminicola. In many biological systems, cAMP is generated by adenylyl cyclase and acts as a second messenger that binds to the regulatory subunit of PKA. This leads to dissociation of the regulatory and catalytic subunits of this complex and consequently activates the latter. Hence, inhibition of adenylyl cyclase by disruption of Gαs genes or the catalytic subunit-encoding gene of PKA could lead to a similar phenotype. Indeed, the MgGpa3 and MgTpk2 (catalytic subunit-encoding gene of PKA) mutants of M. graminicola showed similar phenotypes, including reduced filamentation, increased microconidiation on PDA, and secretion of a dark brown pigment into YGB (43). Therefore, we suggest that MgGPA3 acts upstream of MgTPK2, positively regulates the cAMP pathway, and functionally belongs to the Gαs protein family.
Since MgGPA2 belongs to a distinct group of G proteins (group II) with no mammalian orthologs, we cannot predict its biochemical function yet. Interestingly, the phenotypes of all MgGpa2 mutants were identical to that of the WT or ectopic transformants under all in vitro and in planta conditions tested, which supports observations with U. maydis, Botrytis cinerea, and M. grisea where disruption of the MgGpa2 orthologs did not alter or only slightly affected in vitro phenotypes and pathogenicity (22, 41, 57). The phenotypes of all fungal Gα2-encoding gene mutants belonging to group II obtained so far indicate that this type of Gα protein does not play a major role in development and/or pathogenicity of plant-pathogenic fungi. Further studies are required to understand the role of the GPA2 subunits that are both specific to and highly conserved in filamentous fungi.
Spores of MgGpb1 mutants germinated normally, but the germ tubes had a wavy appearance and showed anastomosis on WA and PDA, which culminated on the latter medium into extensive fusions, resulting in dense areas in the colonies with little filamentous growth and strongly reduced conidiation. Although somatic cell fusion, known as homokaryon anastomosis, is common during vegetative growth of many filamentous fungi (18, 19, 23), it is very unusual for M. graminicola. Here we show that in M. graminicola, MgGpb1 negatively regulates vegetative cell fusion, resulting in its unique phenotype. The molecular mechanism of homokaryon cell fusion is poorly understood, but N. crassa ham-1 and ham-2 mutants were unable to undergo both self- and nonself-hyphal fusion during vegetative growth (69, 70). In S. cerevisiae and Cryptococcus neoformans, Gβ positively regulates the mating pheromone response pathway. Therefore, disruption of the encoding gene inhibits the response to sex pheromones (67, 68). In contrast, in S. pombe pheromone signaling is mediated by Gα (Gpa1) and the cAMP pathway is regulated by Gα (Gpa2) and Gβ (Gpb1). Interestingly, deletion of Gβ in S. pombe stimulates conjugation in nutrient-rich media, which is in accordance with our observations (37, 40). We showed that exogenous cAMP restored the phenotypes of the MgGpa3 and MgGpb1 mutants to the WT phenotype, indicating that these genes positively regulate the cAMP pathway. A possible mechanism for cAMP pathway regulation by MgGPB1 and MgGPA3 in M. graminicola could be that MgGPB1 is required for the efficient release of MgGPA3 from the heterotrimeric complex in order to stimulate adenylyl cyclase. Alternatively, or in addition, MgGPB1 could directly stimulate adenylyl cyclase, as observed for type II mammalian adenylyl cyclase, which is positively regulated by both Gα and Gβγ subunits (13, 15, 64).
We observed that all M. graminicola strains were equally thermosensitive, whereas for N. crassa and F. oxysporum, thermotolerance was observed in Gα-encoding mutants and was positively correlated with decreased intracellular cAMP levels (28, 29, 71). Indeed, constitutive expression of Gna1 in N. crassa resulted in elevated cAMP levels and increased thermosensitivity (26, 71). The observed phenotypic differences could be due to the various lifestyles of these fungi.
Similar to MgGpa1, the orthologous group I Gα proteins in C. parasitica, M. grisea, Stagonospora nodorum, F. oxysporum, Colletotrichum trifolii, and B. cinerea affect pathogenicity (7, 16, 28, 41, 61, 65), whereas the orthologous proteins CGA1 and GPA1 in Cochliobolus heterostrophus and U. maydis, respectively, are dispensable for pathogenicity (24, 57). Such a functional dichotomy was also observed for group III Gα orthologs. M. graminicola MgGpa3 mutants were strongly reduced in pathogenicity, which was also observed in U. maydis and F. oxysporum (29, 57) but not in M. grisea and C. parasitica (7, 41). Similar observations were reported for Gβ-encoding genes. MgGpb1 mutants and orthologous mutants in F. oxysporum, M. grisea, C. parasitica, and C. heterostrophus were impaired in pathogenicity, whereas mutation of the Gβ-encoding gene in U. maydis did not disrupt pathogenicity (14, 27, 31, 48, 52).
Recently, we showed that impaired melanization in M. graminicola correlated with defects in pathogenicity for MgFus3, MgSlt2, and MgHog1 mutants (8, 44, 45), which is in agreement with our present results, where we observed that disruption of the MgGpa1, MgGpb1, and MgGpa3 genes affects melanization as well as pathogenicity. This is somewhat surprising, as M. graminicola penetrates its host by stomata, whereas melanization is reported to be very important for generating turgor pressure in the appressoria of appressorium-forming fungal plant pathogens like M. grisea and Colletotrichum lagenarium (32, 38, 46, 63). The melanin biosynthesis pathway in fungi is also well characterized (47, 66), and expression of three melanin biosynthesis genes was reduced only at the onset of germination in the Fus3 orthologous mutants of C. lagenarium, suggesting the involvement of this ortholog in melanization of appressoria (62). However, unlike the case in appressorium-forming pathogens, melanization in M. graminicola might affect other stages during infection of plants where melanization is important. Indeed, our previous studies revealed that nonmelanized mutants of MgSlt2 and the WT strain were equally effective in penetrating the host plant (44) but differed significantly in pathogenicity, which suggests that melanization is required at the time that the fungus starts to differentiate asexual fructifications in substomatal cavities (11, 36). However, to test this hypothesis, genes involved in melanin biosynthesis need to be studied in detail. In addition, expression profiling of the mutants generated in this study could possibly reveal potential cross talk between the melanin biosynthesis and cAMP pathways in M. graminicola.
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TABLE 1.
| Gene or position | M. graminicola transformant | Type | Primer | Primer sequence |
|---|---|---|---|---|
| MgGpa1 | IPO323 ΔMgGpa1-14 | Disruption strain | MgGpa1-F3 | TTCAGCGAAGCCGAGAATATC |
| IPO323 ΔMgGpa1-15 | Disruption strain | MgGpa1-R2 | CACATGCGCAGGTTCTCTTG | |
| IPO323 E-MgGpa1-13 | Ectopic transformant | |||
| MgGpa2 | IPO323 ΔMgGpa2-2 | Disruption strain | MgGpa2-F1 | GGCGTACAGCTTGCGAT |
| IPO323 ΔMgGpa2-6 | Disruption strain | MgGpa2-R2 | CATCAAGTTATGCAAGTTCCGC | |
| IPO323 E-MgGpa2-10 | Ectopic transformant | |||
| MgGpa3 | IPO323 ΔMgGpa3-21 | Disruption strain | MgGpa3-F2 | AGCAGTGGGAACAATGAGGAG |
| IPO323 ΔMgGpa3-33 | Disruption strain | MgGpa3-R4 | TGAATACTGAGCTGGCCCAT | |
| IPO323 E-MgGpa3-2 | Ectopic transformant | |||
| MgGpb1 | IPO323 ΔMgGpb1-3 | Disruption strain | MgGpb1-F4 | ACCACGAACAAAGTACACGCC |
| IPO323 ΔMgGpb1-22 | Disruption strain | MgGpb1-R4 | AGTCGACCTGAGACGGAGAAAG | |
| IPO323 E-MgGpb1-1 | Ectopic transformant | |||
| Left border of transposon (Tn7L) | S | ATAATCCTTAAAAACTCCATTTCCACCCCT | ||
| Right border of transposon (Tn7R) | N | ACTTTATTGTCATAGTTTAGATCTATTTTG |
TABLE 2.
| Mycosphaerella graminicola isolate | Intracellular cAMP value (pmol cAMP/ mg total protein)a | Phenotype on PDA | Phenotype on PDA plus cAMP |
|---|---|---|---|
| Controls | |||
| IPO323 | Short germ tubes | Short germ tubes | |
| IPO323 E-MgGpa | 10.17c | Budding on apical and intermediate cells of spores and secondary filaments (24 h) | Budding on apical and intermediate cells of spores and secondary filaments (24 h) |
| IPO323 E-MgGpb | Filamentation (48 h) | Reduced filamentation (48 h) | |
| Microconidiation in center of colony (80 h) | Microconidiation in center of colony (80 h) | ||
| Mutants | |||
| IPO323 ΔMgGpa1 | 9.45bc | Long germ tubes | Reduced filamentation |
| Increased filamentation, reduced branching (48 to 80 h) | |||
| IPO323 ΔMgGpb1 | 5.48b | Fused germ tubes (48 h) | Restored to control phenotype |
| Uncontrolled anastomosis (80 h) | |||
| IPO323 ΔMgGpa3 | 1.68a | Short germ tubes | Restored to control phenotype |
| No secondary and tertiary filaments | |||
| Extensive budding |
a
Back-transformed values. Values with the same letter are not significantly different at a P level of 0.05.
TABLE 3.
| Organism | Gene | Class | Growth | Conidiation | Germination | Virulence | Additional phenotypes | Reference |
|---|---|---|---|---|---|---|---|---|
| C. heterostrophus | Cga1 | Gα-I | Reduced | Reduced | Normal | Low appressorium formation, osmosensitive | 24 | |
| C. trifolii | Ctg1 | Gα-I | Reduced | Reduced | Reduced | Low appressorium formation, long germ tubes bearing appressoria | 65 | |
| C. parasitica | Cpg-1 | Gα-I | Reduced | Lost | Lost | Reduced pigmentation, increased cAMP level | 16 | |
| F. oxysporum | Fga1 | Gα-I | Normal | Reduced | Reduced | Decreased cAMP level, thermotolerant | 27 | |
| M. grisea | MagB | Gα-I | Reduced | Reduced | Normal | Reduced | Reduced penetration, long germ tubes bearing appressoria that are restored by exogenous cAMP | 41 |
| S. nodorum | Gna1 | Gα-I | Normal | Lost | Reduced | Defect in penetration, albino, osmosensitive | 61 | |
| U. maydis | Gpa1 | Gα-I | Normal | Normal | Normal | Normal | No phenotype in all aspects | 57 |
| B. cinerea | Bcg1 | Gα-I | Reduced | Normal | Normal | Reduced | Altered colony morphology, normal penetration, loss of protease secretion, | 22 |
| M. graminicola | Gpa1 | Gα-I | Normal | Reduced | Normal | Reduced | Altered colony morphology, hampered melanization, induced filamentation, altered polarized growth | This study |
| C. parasitica | Cpg-3 | Gα-II | Not functionally analyzed | 53 | ||||
| M. grisea | MagC | Gα-II | Normal | Reduced | Normal | Normal | Normal appressorium formation | 41 |
| U. maydis | Gpa2 | Gα-II | Normal | Normal | Normal | Normal | No phenotype in all aspects | 57 |
| B. cinerea | Bcg2 | Gα-II | Normal | Normal | Normal | Reduced | Normal colony morphology, virulence slightly reduced | 22 |
| M. graminicola | Gpa2 | Gα-II | Normal | Normal | Normal | Normal | No phenotype in all aspects | This study |
| C. parasitica | Cpg-2 | Gα-III | Reduced | Reduced | Normal | Decreased cAMP level | 16 | |
| F. oxysporum | Fga2 | Gα-III | Normal | Normal | Lost | Thermotolerant | 29 | |
| M. grisea | MagA | Gα-III | Normal | Normal | Normal | Normal | No phenotype in all aspects | 41 |
| U. maydis | Gpa3 | Gα-III | Lost | Elongated cells in liquid media, defective in mating | 57 | |||
| M. graminicola | Gpa3 | Gα-III | Reduced | Increased | Normal | Reduced | Altered colony morphology, hampered filamentation, reduced melanization | This study |
| U. maydis | Gpa4 | Gα-IV | Normal | Normal | Normal | Normal | No phenotype in all aspects | 57 |
| F. oxysporum | Fgb1 | Gβ | Reduced | Reduced | Normal | Reduced | Altered colony morphology, faster conidial germination, decreased cAMP level, thermotolerant | 27 |
| M. grisea | Mgb1 | Gβ | Reduced | Reduced | Delayed | Lost | No appressorium formation, decreased cAMP level, more aerial hyphae, formation of fluffy colonies | 52 |
| C. parasitica | Cpgb-1 | Gβ | Increased | Reduced | Reduced | Reduced pigmentation | 31 | |
| C. heterostrophus | Cgb1 | Gβ | Normal | Lost | Lost | Impaired formation of appressoria, increased pigmentation, straight germination | 14 | |
| U. maydis | Bpp1 | Gβ | Normal | Increased filamentation and reduced budding growth, restored by adding exogenous cAMP | 48 | |||
| M. graminicola | Gpb1 | Gβ | Reduced | Reduced | Normal | Reduced | Altered colony morphology, hampered filamentation and melanization, increased anastomosis | This study |
Acknowledgments
Rahim Mehrabi was financially supported by the Agricultural Research and Education Organization (AREO) of Iran. The construction of EST libraries was funded by Syngenta, Jealott's Hill, United Kingdom. Gert H. J. Kema is a recipient of an OECD fellowship to USDA-ARS at Purdue University, West Lafayette, IN. Sarrah Ben M'Barek is cosponsored by a UNESCO-L'Oréal fellowship.
The Dutch Mycosphaerella group and Maarten A. De Waard are gratefully acknowledged for discussions and suggestions. Gert H. J. Kema gratefully acknowledges discussions with Steve Goodwin and Jin-Rong Xu on cell biological aspects of M. graminicola. We thank Jacques Withagen (Wageningen University and Research Center- Biometris) for statistical advice and Wouter Van Egmond and Peter Van Haastert at the University of Groningen for help with the cAMP assay. We thank two anonymous reviewers for useful suggestions that improved the manuscript.
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Received: 1 August 2008
Accepted: 17 April 2009
Published online: 1 July 2009
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