RESULTS AND DISCUSSION
Phylogenetic analysis of the PACE family.
Expression of PA2880 in E. coli leads to a higher cellular resistance to chlorhexidine but not cadaverine.
|Protein expressed||MIC ofa:|
|Chlorhexidine (μg/mL)||Cadaverine (mg/mL)|
Efflux of chlorhexidine mediated by PA2880 is driven by proton motive force.
Chlorhexidine/H+ antiport is an electrogenic process.
The oligomeric state of PA2880 in solution.
MATERIALS AND METHODS
Construction of the expression vector.
Electroporation of Pseudomonas stutzeri.
Protein expression and purification.
Antimicrobial susceptibility test.
Reconstitution of protein PA2880 and its variants.
Chlorhexidine/H+ antiport assays using proteoliposomes containing pyranine.
Electrogenicity experiments of PA2880.
Polyacrylamide gel electrophoresis and Western blotting.
Peer Review History
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