Gemykibivirus detection in acute encephalitis patients from Nepal
ABSTRACT
IMPORTANCE
INTRODUCTION
RESULTS
Detection of a gemykibivirus by metagenomic NGS


Identification of additional positive specimens from encephalitis patients
Sample ID | Specimen | Age | Gender | Ct. Gemy_1a | Ct. Gemy_2b | Specimen collection date | Districtc | Province |
---|---|---|---|---|---|---|---|---|
N0000218 | CSF | 14 yrs | M | 32.42 | 30.68 | 2020 Feb | Kaski | Gandaki |
N0000256 | CSF | 9 mos | F | 29.41 | 29.34 | 2020 July | Myagdi | Gandaki |
N0000300 | CSF | 8 yrs | F | 32.95 | 31.46 | 2020 Sep | Palpa | Lumbini |
N0000358 | Serum | 6 mos | M | 29.27 | 29.94 | 2022 Feb | Sarlahi | Madhesh |
N0000359 | CSF | 5 yrs | F | 27.4 | 27.08 | 2022 Feb | Kapilvastu | Lumbini |
N0000395 | CSF | 4 mos | M | 30.74 | 30.73 | 2022 April | Kaski | Gandaki |
N0000434 | CSF | 57 yrs | M | 29.02 | 30.06 | 2022 June | Lalitpur | Bagmati |
N0000468 | CSF | 35 yrs | F | 30.84 | 31.47 | 2022 July | Chitwan | Bagmati |
N0000490 | CSF | Unknown | M | 29.86 | 32.08 | 2022 July | Syangja | Gandaki |
N0000545 | CSF | 72 yrs | F | 26.84 | 25.35 | 2022 Aug | Kathmandu | Bagmati |
N0000546 | CSF | 21 yrs | M | 24.65 | 23.53 | 2022 Aug | Kathmandu | Bagmati |
N0000722 | CSF | 25 yrs | F | 28.74 | 29.72 | 2022 Nov | Chitwan | Bagmati |

Whole genome sequencing and phylogenetic analysis of additional positive cases
DISCUSSION
MATERIALS AND METHODS
Study population and collection of biospecimens
Total nucleic acid extraction
Metagenomic NGS analysis
Genome sequencing of index case and additional cases
Gemykibivirus qPCR development
Phylogenetic analysis
ACKNOWLEDGMENTS
SUPPLEMENTAL MATERIAL
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