Screening the UCSF 2015 and 2016 deletion mutant libraries for caspofungin sensitivity.
Confirmation of echinocandin sensitivity phenotypes.
|Plate||Accession no.a||Gene||Protein description|
|0-B12||CNAG_06242||CFT1||High-affinity ferric transporter|
|5-F6||CNAG_02830||ERG4||Delta24(24(1))-sterol reductase; ergosterol biosynthesis|
|23-C7||CNAG_01536||MYO1||Myosin heavy chain|
|34-F12||CNAG_00650||YSP2||StART family protein involved in sterol transport|
|38-C7||CNAG_06376||PEP5||Vacuolar protein sorting and E3 ubiquitin ligase|
Stress and antifungal phenotypes of the new deletion mutant strains.
Chitin and chitosan levels and localization.
Morphology of cell walls.
Membrane visualization by fluorescent dyes.
Comparison to other caspofungin screens.
Impact of caspofungin on iron uptake.
Caspofungin and ergosterol synthesis.
Caspofungin and myosin.
Caspofungin and sterol transport.
MATERIALS AND METHODS
Strains, media, and chemicals.
Screening for caspofungin sensitivity.
Generation of gene deletion strains.
Agar plate phenotyping.
Determination of MIC.
Transmission electron microscopy.
Sterol quantification method.
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