ANNOUNCEMENT
Plant rhizospheres have long been investigated and exploited for their plant growth-promoting (PGP) rhizobacteria (
1,
2). Potato tubers and rhizosphere soils were sampled from the Tanzanian northern and southern highlands for rhizobacterial isolation (
3,
4).
Klebsiella sp. strain MPUS7,
Serratia sp. strain NGAS9, and
Citrobacter sp. strain LUTT5, identified by partial 16S rRNA gene sequencing (
5), were selected for whole-genome sequencing.
The strains were grown in Trypticase soy broth (Difco) at 37 ± 2°C for 24 h in a rotary shaker (130 rpm). Total nucleic acids were extracted with the MasterPure DNA purification kit (Epicentre, Madison, WI, USA) and used for sequence library preparations without further processing. Illumina paired-end shotgun libraries were generated with the Nextera XT DNA sample preparation kit and sequenced using the MiSeq system and reagent kit v.3 (2 × 300 bp) (Illumina, San Diego, CA, USA). For Nanopore sequencing, libraries were prepared using the ligation sequencing kit 1D (SQK-LSK108) and the native barcode expansion kit (EXP-NBD103) (Oxford Nanopore Technologies, Oxford, UK). Sequencing was performed for 72 h on a MinION Mk1B device and a SpotON flow cell R9.4 using MinKNOW software v.19.06.8 (Oxford Nanopore Technologies). The short and long reads were called with the MiSeq control software v.2.6.2.1 and Guppy v.3.2.1., respectively. Read quality assessment and processing were performed with fastp v.0.19.5 (
6), resulting in 2,304,340, 2,623,096, and 2,439,948 short Illumina reads and 26,946 (
N50, 19.4 kb), 34,866 (
N50, 19.9 kb), and 31,389 (
N50, 20 kb) long Nanopore reads for
Klebsiella sp. MPUS7,
Serratia sp. NGAS9, and
Citrobacter sp. LUTT5, respectively. All kits were used as recommended by the manufacturers, and default parameters were used for all software unless otherwise specified.
Genome assemblies were performed using the Unicycler v.0.4.8 (
7) pipeline with SPAdes v.3.14.0 (
8), Racon v.1.4.10 (
9), BLAST v.2.2.28+ (
10), Bowtie 2 v.2.3.4.3 (
11), SAMtools v.1.9 (
12), and Pilon v.1.23 (
13) and resulted three times in single circular chromosomes. The Unicycler pipeline automatically rotated all genomes, defining
dnaA as the first protein-coding gene. The average coverage was calculated with Qualimap v.2.2.1 (
14); Bowtie 2 v.2.3.4.3 (
11) was used for short-read mapping, and Minimap2 v.2.17 (
15) was used for long-read mapping. This resulted in 74-, 97-, and 61-fold (Illumina reads) and 121-, 216-, and 208-fold (Nanopore reads) genome mean coverage from
Klebsiella sp. MPUS7,
Serratia sp. NGAS9, and
Citrobacter sp. LUTT5, respectively. BLAST analysis of the complete 16S rRNA genes of these strains showed over 99.6% similarity to
Klebsiella grimontii SB73 (GenBank accession number
NR_159317.1),
Serratia marcescens NBRC 102204 (
NR_114043.1), and
Citrobacter freundii ATCC 8090 = MTCC 1658 (
NR_028894.1), respectively. Gene annotation was done with the Prokaryotic Genome Annotation Pipeline v.4.8 (
16).
The genome features of the strains are summarized in
Table 1. Their protein-encoding genes included potassium, nitrogen, phosphorus, and iron metabolism genes, which are associated with plant growth promotion (
17–19). These genomes are the first to be sequenced for potato rhizobacteria in Tanzania and can help to unravel their molecular PGP mechanisms for possible biotechnological application as biofertilizers.
Data availability.
The whole-genome shotgun projects of
Klebsiella sp. MPUS7,
Serratia sp. NGAS9, and
Citrobacter sp. LUTT5 have been deposited at GenBank under the accession numbers
CP047604,
CP047605, and
CP047606, respectively. The versions described here are the first versions. The raw sequencing data sets of these strains have been registered in the NCBI Sequence Read Archive database (
20) under the accession numbers
SRP255262,
SRP255259, and
SRP255263, respectively.
ACKNOWLEDGMENTS
This work was supported by grants from the German Federal Ministry of Education and Research at the Department of Genomics and Applied Microbiology of the Institute of Microbiology and Genetics at the Georg-August University of Göttingen in Germany. The APC was funded by the L’Oreal-UNESCO Foundation for Women in Science. The funders had no role in the study design, data collection and interpretation, or the decision to submit the work for publication.
We thank Anja Poehlein of the Georg-August University for sequencing and handling the Illumina data.