Open access
Antimicrobial Chemotherapy
Announcement
1 April 2021

Draft Genome Sequences and Antimicrobial Profiles of Three Staphylococcus epidermidis Strains from Neonatal Blood Samples

ABSTRACT

Data on molecular characterization of coagulase-negative staphylococci causing neonatal sepsis in low-income countries are highly limited. This report highlights the isolation of three Staphylococcus epidermidis non-genome assembly strains (NGASs) from blood samples from neonates with unknown transmission sources. Pathogenic factors and sources of transmission of these strains warrant further investigation.

ANNOUNCEMENT

We report the draft genome sequences of three Staphylococcus epidermidis non-genome assembly strains (NGASs) cultivated from blood samples obtained from neonates at the Ho Teaching Hospital (HTH) in Ghana. S. epidermidis is a coagulase-negative staphylococcus (CoNS), a permanent member of the human microbiota that commonly colonizes the skin and mucous membranes (1). S. epidermidis has become an important opportunistic pathogen among vulnerable patients, and it is reported to be a leading causative agent of nosocomial infections (2). S. epidermidis is the most prevalent CoNS commonly isolated from bloodstream infections in neonatal intensive care units (NICUs) (3). We have isolated, sequenced, and assembled three S. epidermidis NGASs from neonatal blood samples. Molecular typing of the tuf gene revealed them (of 74 isolates cultured from the HTH environment) to be peculiar strains isolated from blood culture samples; they had the highest levels of identity to a strain deposited in GenBank (accession number LR735440.1). Their transmission source was unclear, which prompted us to study these strains in more detail and to sequence their whole genomes. The Research Ethics Committee of the University of Health and Allied Sciences (Ho, Ghana) reviewed and approved this study (protocol UHAS-REC/A.2[1]17-18). Written approval was obtained from the HTH to use the facility for the study.
Blood samples were cultured using a Bactec 9050 system from March to June 2018. Positive culture bottles were subcultured on 5 to 10% sheep blood agar, harvested, and stored (in glycerol broth) at −20°C for 1 month before being shipped to Germany. Isolates were revived on blood agar plates incubated at 37°C for 24 h. Isolates were identified with a matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) Biotyper G (Bruker Daltonics, Billerica, MA, USA). The MICs of 17 antibiotics were determined with a Vitek 2 system (bioMérieux, Durham, NC, USA). Genomic DNA was extracted using the DNeasy blood and tissue kit (Qiagen, Germany). Novogene (UK) Ltd. performed whole-genome sequencing. Library preparation was performed with the NEBNext Ultra DNA library preparation kit (New England Biolabs, Ipswich, MA, USA) according to the manufacturer's directions. The genomic DNA was randomly fragmented to a size of 350 bp by shearing. DNA fragments were end polished, A tailed, and ligated with the NEBNext adapter for Illumina sequencing and were further PCR enriched with P5 and indexed P7 oligonucleotides. The PCR products were purified (AMPure XP system), and the resulting libraries were analyzed for size distribution with an Agilent 2100 Bioanalyzer and quantified using real-time PCR. Library validation was performed on a Fragment Analyzer system (Agilent Technologies, CA) to control library quality, and quantification was performed using quantitative PCR via an ABI QuantStudio 12K Flex system (Thermo Fisher Scientific). Whole-genome sequencing was performed with an Illumina NovaSeq 6000 system to generate 150-bp paired-end reads. The genomes were sequenced at a minimum coverage of 100×. FASTQ files were processed to remove adaptor sequences, to trim low-quality ends, and to remove short reads using fastp v0.20.0 (3). To validate the isolates’ purity, the isolates were taxonomically classified on the basis of the sequencing reads using Kraken v2 (4) with the default taxonomy database. Sequencing reads were assembled using MEGAHIT v1.2.9 (5). Gene prediction and functional annotation were performed using Prokka v1.14.6 (6) with the BLAST, Pfam, and NCBI databases. The aforementioned software packages were used with default parameters.
A multilocus sequence typing (MLST) online search (https://cge.cbs.dtu.dk/services/MLST-2.0) was performed as described previously (7). Antibiotic resistance genes were searched for with the CARD Resistance Gene Identifier (https://card.mcmaster.ca/analyze/rgi) and IHU-Méditerranée Infection (https://ifr48.timone.univ-mrs.fr/blast/arg-annot_v6.html) websites. The staphylococcal cassette chromosome mec (SCCmec) type was determined with SCCmecFinder v1.2 (www.cge.cbs.dtu.dk/services/SCCmecFinder). Table 1 summarizes the results.
TABLE 1
TABLE 1 Genome assemblies and antimicrobial susceptibility patterns of three Staphylococcus epidermis strains, HESN038B, HESN074B, and hHESN103B
ParameterData for strainb:
HESN038BHESN074BhHESN103B
MIC (mg/liter) (susceptibility
 category)a
 Cefoxitin2 (negative)2 (negative)>8 (positive)
 Oxacillin0.25 (NA)2 (NA)>2 (NA)
 Erythromycin0.25 (S)0.5 (S)>2 (R)
 Clindamycin0.25 (S)0.25 (S)>1 (R)
 Amikacin4 (S)8 (S)8 (S)
 Ampicillin2 (NA)2 (NA)>8 (NA)
 Gentamicin1 (S)>4 (R)>4 (R)
 Tetracycline0.5 (S)0.5 (S)>2 (R)
 Fosfomycin with G6Pc16 (S)16 (S)16 (S)
 Ciprofloxacin0.25 (S)0.25 (S)4 (S)
 Rifampin0.25 (S)0.25 (S)0.25 (S)
 Trimethoprim-sulfamethoxazole4/76 (S)>4/76 (R)>4/76 (R)
 Tobramycin1 (S)>4 (R)>4 (R)
 Penicillin G0.0625 (NA)>0.25 (NA)>0.25 (NA)
 Teicoplanin1 (S)1 (S)1 (S)
 Vancomycin1 (S)0.5 (S)1 (S)
 Mupirocin1 (S)1 (S)1 (S)
Resistance genesdfrC, norA, fosB,
gyrB, IS1272
dfrC, norA, fusB, far1,
gyrB, (AGly)apH-stph, mecA
dfrC, dfrG, norA,
gyrB, IS1272
Genome size (bp)2,682,6782,439,2542,431,156
No. of contigs1153025
Size of largest contig (bp)297,254335,917569,583
No. of reads5,934,9136,679,8845,798,804
N50 (bp)78,190148,419233,414
GC content (%)31.832.0132
No. of rRNAs888
No. of coding sequences2,5082,2382,233
No. of tRNAs515249
No. of transfer-messenger RNAs011
MLST sequence typeUnknownUnknown226
SCCmec typeNoneV (5C2&5)None
BioProject accession no.PRJNA668279PRJNA668279PRJNA668279
GenBank accession no.JADCSK000000000JADCSI000000000.1JADCSS000000000.1
BioSample accession no.SAMN16402351SAMN16402349SAMN16402359
SRA accession no.SRR13236751SRR13236760SRR13236759
a
The MIC results were interpreted according to 2018 European Committee on Antimicrobial Susceptibility Testing (EUCAST) clinical breakpoints (https://www.eucast.org/clinical_breakpoints).
b
R, resistant; S, susceptible; NA, not applicable (no breakpoint available); V (5C2&5), type V SCCmec combination of the ccr gene complex (type 1) and mec gene complex class 2.
c
G6P, glucose 6-phosphate.

Data availability.

This whole-genome shotgun project has been deposited in DDBJ/ENA/GenBank under the accession numbers listed in Table 1.

ACKNOWLEDGMENTS

The Ph.D. candidate (Innocent Afeke) on this project was sponsored by the Ghanaian-German Postgraduate Training Program 2017, DAAD (grant 91652994). The DFG, University of Lübeck, supported the research work.
No competing interests are declared.

REFERENCES

1.
Becker K, Heilmann C, Peters G. 2014. Coagulase-negative staphylococci. Clin Microbiol Rev 27:870–926.
2.
Arora S, Li X, Hillhouse A, Konganti K, Little SV, Lawhon SD, Threadgill D, Shelburne S, Hook M. 2020. Staphylococcus epidermidis MSCRAMM SesJ is encoded in composite islands. mBio 11:e02911-19.
3.
Chen S, Zhou Y, Chen Y, Gu J. 2018. fastp: an ultra-fast all-in-one FASTQ preprocessor. Bioinformatics 34:i884–i890.
4.
Wood DE, Lu J, Langmead B. 2019. Improved metagenomic analysis with Kraken 2. Genome Biol 20:257.
5.
Li D, Liu CM, Luo R, Sadakane K, Lam TW. 2015. MEGAHIT: an ultra-fast single-node solution for large and complex metagenomics assembly via succinct de Bruijn graph. Bioinformatics 31:1674–1676.
6.
Seemann T. 2014. Prokka: rapid prokaryotic genome annotation. Bioinformatics 30:2068–2069.
7.
Larsen MV, Cosentino S, Rasmussen S, Friis C, Hasman H, Marvig RL, Jelsbak L, Sicheritz-Pontén T, Ussery DW, Aarestrup FM, Lund O. 2012. Multilocus sequence typing of total-genome-sequenced bacteria. J Clin Microbiol 50:1355–1361.

Information & Contributors

Information

Published In

cover image Microbiology Resource Announcements
Microbiology Resource Announcements
Volume 10Number 131 April 2021
eLocator: 10.1128/mra.00170-21
Editor: Julie C. Dunning Hotopp, University of Maryland School of Medicine

History

Received: 15 February 2021
Accepted: 9 March 2021
Published online: 1 April 2021

Contributors

Authors

Luebeck Institute for Experimental Dermatology, University of Luebeck, Luebeck, Germany
Department of Medical Laboratory Sciences, School of Allied Health Sciences, University of Health and Allied Sciences, Ho, Ghana
Ahmed Moustafa
Department of Biology, Bioinformatics, and Integrative Genomics Laboratory, American University in Cairo, New Cairo, Egypt
Misa Hirose
Luebeck Institute for Experimental Dermatology, University of Luebeck, Luebeck, Germany
Mareike Becker
Luebeck Institute for Experimental Dermatology, University of Luebeck, Luebeck, Germany
Hauke Busch
Luebeck Institute for Experimental Dermatology, University of Luebeck, Luebeck, Germany
Axel Kuenstner
Luebeck Institute for Experimental Dermatology, University of Luebeck, Luebeck, Germany
Anke Faehnrich
Luebeck Institute for Experimental Dermatology, University of Luebeck, Luebeck, Germany
Anthony S. Ablordey
Department of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra, Ghana
Christoph Haertel
Department of Pediatrics, Wuerzburg University Clinic, University of Wuerzburg, Wuerzburg, Germany
Kokou Hefoume Amega-Aho
Department of Pediatrics, School of Medicine, University of Health and Allied Sciences, Ho, Ghana
Mohamed Tarek Badr
Institute of Medical Microbiology and Hygiene, Medical Center-University of Freiburg, Faculty of Medicine, Freiburg, Germany
Clinic for Infectiology and Microbiology, University of Luebeck, Luebeck, Germany
Saleh Ibrahim
Luebeck Institute for Experimental Dermatology, University of Luebeck, Luebeck, Germany

Editor

Julie C. Dunning Hotopp
Editor
University of Maryland School of Medicine

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