Coatomer complex I is required for the transport of SARS-CoV-2 progeny virions from the endoplasmic reticulum-Golgi intermediate compartment
ABSTRACT
IMPORTANCE
INTRODUCTION
RESULTS
Three-dimensional ultrastructural analysis of SARS-CoV-2-infected cells


Involvement of COPI in the vesicular transport of SARS-CoV-2 virions

Category | Gold signal | χ2 | P value | |||||
---|---|---|---|---|---|---|---|---|
Positive | Negative | Total count | ||||||
Count | % | Count | % | |||||
β-COP | Vesicles and coated pits in uninfected cells | 5 | 10 | 45 | 90 | 50 | 44.43 | <0.00001 |
Virion-containing vesicles and coated pits in infected cells | 38 | 76 | 12 | 24 | 50 | |||
ERGIC-53 | Vesicles and coated pits in uninfected cells | 5 | 10 | 45 | 90 | 50 | 41.50 | <0.00001 |
Virion-containing vesicles and coated pits in infected cells | 37 | 74 | 13 | 26 | 50 |
COPI facilitates the release of virion-containing vesicles from the ERGIC

DISCUSSION

MATERIALS AND METHODS
Cells and virus
Antibodies
Virus titration
Immunofluorescence assay
Sample preparation for electron microscopy
Ultrathin-section TEM, electron tomography, and immunoelectron microscopy
SEM array tomography
siRNA treatment
Cell viability assay
Western blotting
Statistical analysis
ACKNOWLEDGMENTS
SUPPLEMENTAL MATERIAL
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