Computational Analysis Reveals a Key Regulator of Cryptococcal Virulence and Determinant of Host Response

Capsule might be regulated at multiple levels. These could include mechanisms involving transcription, mRNA (stability or localization), or posttranslational processes (such as modification or relocalization of proteins involved in capsule biosynthesis). To test whether new protein synthesis is required for capsule growth, we induced cells to enlarge their capsules in the absence or presence of cycloheximide (CHX), which inhibits protein synthesis by preventing translational elongation, and then measured capsule thickness. (Representative images are shown in Fig. 1, and population measurements are provided in Table S1A in the supplemental material.) Cells exposed to CHX throughout a 24-h period of capsule induction showed severely impaired capsule growth compared to untreated cells (Fig. 1, top row, compare first and third images); if CHX was introduced partway through induction (at 8 h), the cells generated capsules of the size expected from only the initial induction period (Fig. 1, top row, second image). Finally, the inhibition of capsule growth was reversible by the restoration of protein synthesis, as shown by cells treated for 8 h with CHX and then washed into medium without drug for the remaining 16 h of induction (Fig. 1, top row, fourth image). Notably, these cells displayed slightly larger capsules than untreated cells, consistent with a “rebound” effect potentially due to mRNA accumulation during CHX treatment. These results strongly suggest that new protein synthesis is indeed required for capsule enlargement, although they do not rule out indirect effects of CHX on secretory vesicles (11), which have been implicated in capsule synthesis (12).

Since capsule regulation occurs upstream of protein synthesis, it is likely to act at the transcriptional level. However, other upstream steps, such as regulation of translation, mRNA stability, or mRNA localization, might also be sufficient for capsule induction. To test whether new transcription is required, we treated cells with 1,10-phenanthroline (PHN), an inhibitor of RNA synthesis. The results with this compound (Fig. 1, bottom row) were qualitatively similar to those of the CHX experiment, except that treated cells restored to drug-free medium did not generate full-size capsules. This may be because inhibition of transcription is a more drastic insult to the cells, from which they are slow to recover. In any event, these studies support our working model that capsule synthesis requires both mRNA and protein synthesis, suggesting that the dominant regulatory influences on capsule occur at the level of transcription.

Usv101 is predicted to be a DNA-binding protein. Consistent with this, a C-terminal hemagglutinin (HA)-tagged version of the protein localized to the nucleus by immunofluorescence microscopy, whether cells were grown in non-capsule-inducing (yeast extract-peptone-dextrose [YPD]) or capsule-inducing (Dulbecco’s modified Eagle’s medium [DMEM]) conditions (not shown). For studies of Usv101 function, we complemented our transcriptome sequencing (RNA-seq)-confirmed usv101Δ mutant (8) with the unmodified wild-type gene at the endogenous genomic locus (USV101 strain); we also generated a Usv101-overexpressing (USV101_OE) strain by replacing the endogenous promoter with that of ACT1. Negative staining showed that the hypercapsular phenotype of the usv101Δ mutant was corrected when the gene was complemented and that the capsule size of the overexpression strain was reduced compared to that of the wild type (Fig. 2A; see Table S1B in the supplemental material). Together, these observations support the action of Usv101 as a repressor of capsule expansion. Interestingly, the increase in usv101Δ capsule size is accompanied by a decrease in capsule polysaccharide that is shed into the medium, a trait we had observed earlier (8) and here quantitated for the mutant, complemented, and overexpressor strains (see Fig. S2A in the supplemental material).

USV101 has two orthologs in S. cerevisiae, USV1 (alias NSF1) and RGM1, which are paralogs of each other resulting from a whole-genome duplication along the S. cerevisiae lineage (13). The three genes are most similar in their C₂H₂ zinc finger DNA binding domains. Rgm1 is a TF that is involved in cell growth (14), activates genes involved in central carbon metabolism, and regulates expression of Y′ telomeric elements and subtelomeric genes (SGD project; http://www.yeastgenome.org/download-data/ [15 July 2015]). Usv1 is involved in energy metabolism and directly regulates genes in response to nutrient limitation conditions (15). It binds to promoter regions of genes involved in general responses to stresses, including heat shock, oxidative stress (16), and high salt (17). Consistent with this role, S. cerevisiae strains lacking USV1 are sensitive to stresses, including ethanol, salt, and high concentrations of glucose (18–20). We next tested whether the cryptococcal Usv101 was also involved in the regulation of stress responses.

Although the usv101Δ mutant showed a subtle growth defect compared to the wild type on rich medium (YPD) at both 30 and 37°C, it grew normally under multiple conditions that challenge cell integrity (not shown), including media containing Congo red (0.5%), SDS (0.02%), sorbitol (1.5 M), or caffeine (0.05%) (Fig. 2B). Interestingly, although the S. cerevisiae usv1Δ mutant (17) is sensitive to salt, the usv101Δ mutant was more resistant than the wild type to high NaCl (1.2 M) at 37°C; the USV101 overexpression strain also exhibited a subtle sensitivity to high salt (Fig. 2B). Finally, the usv101Δ mutant was sensitive to 6% ethanol (similar to the S. cerevisiae usv1Δ mutant [20]), and the overexpression (USV101_OE) strain was sensitive to 0.05% caffeine (Fig. 2B).

We next tested the ability of the usv101Δ mutant and control strains to withstand nitrosative and oxidative stresses and produce melanin, traits that are associated with resistance to environmental stress and increased virulence in C. neoformans (21–23). Growth of the usv101Δ and USV101_OE strains was not affected by H₂O₂ (not shown), but the deletion mutant was defective in melanin production, as shown by both colony pigmentation and quantitative assay (Fig. 2C). The usv101Δ mutant did show slight sensitivity to nitrosative stress compared to wild-type cells (see Fig. S2B in the supplemental material); this sensitivity was not corrected by melanin induction prior to spotting (see Fig. S2B [data not shown]).

The phenotypic changes in virulence traits exhibited by usv101Δ suggested that this strain would be altered in its interactions with host cells during infection. Critical in these interactions are phagocytes, which have a complex relationship with this facultative intracellular pathogen: while they oppose infection by destroying engulfed cryptococci and helping orchestrate antifungal host responses, they may also promote infection by providing a haven for the fungi in a hostile environment. Either of these outcomes, however, begins with engulfment of C. neoformans. To assess this interaction, we used an automated image-based assay to measure fungal uptake by human THP-1 cells (24). Surprisingly, the engulfment of usv101Δ cells was significantly higher (and that of USV101_OE cells was lower) than those of wild-type and complemented controls (Fig. 3A). This was the opposite of what we expected, since larger capsules generally inhibit phagocytosis (24). Once internalized, however, viability of the internalized fungi was similar for all strains (not shown).

A second key host interaction occurs between C. neoformans and brain microvascular endothelial cells (BMECs) of the blood-brain barrier (BBB), which the pathogen must cross to cause meningoencephalitis. We assayed this transit in vitro, using model BBBs grown in Transwell plates. Notably, the usv101Δ strain was significantly impaired in BBB crossing compared to the wild type and the complemented strain (Fig. 3B); as with capsule size and uptake, the phenotype of the USV101_OE strain in this assay differed from that of the wild type in the opposite direction, crossing into the brain more efficiently than wild type and the complemented mutant.

The reduced virulence of cryptococcal strains with decreased or absent capsule (25) has suggested that hypercapsular mutants would show increased virulence. Contrary to these expectations, however, we recently reported that multiple strains with enlarged capsule, including the usv101Δ mutant, are impaired in growth in short-term animal models of infection (8). These studies were performed with mice that were infected intranasally and then sacrificed 1 week later to assess lung burden: at this time of infection, C. neoformans has not yet disseminated to the brain at detectable levels. In these experiments, the lung burden of usv101Δ cells was almost 2 orders of magnitude lower than that of wild type at the 1-week time point (8), suggesting that the mutant strain would likely be cleared and consequently avirulent. To test this we performed a long-term virulence study, comparing the usv101Δ strain to its wild-type parent, KN99α, the USV101 and USV101_OE strains, and the acapsular (and avirulent) cap59Δ strain. As expected, all mice infected with the wild type, complemented mutant, and overexpression strains succumbed to the infection by 3 weeks postinoculation, while animals infected with the cap59Δ strain survived for the duration of the experiment (Fig. 4A). Surprisingly, although mice infected with the usv101Δ strain remained apparently healthy until week 6, they began to lose weight thereafter. Along with weight loss, these mice developed respiratory symptoms (rapid and labored breathing) but were otherwise alert and mobile; this behavior was in sharp contrast to that of wild-type-infected mice, which typically exhibit neurological symptoms (poor balance and lethargy) late in the course of infection. Together, these observations suggested a fundamental difference between the progression of disease caused by C. neoformans lacking Usv101 and the disease progression caused by the wild type.

To further probe the pattern of usv101Δ pathogenesis, we measured fungal burden over time in the lung, brain, and spleen of mice infected with the wild-type or usv101Δ mutant strain (Fig. 4B). At the earliest time point, 5 days after intranasal infection, the level of usv101Δ cells in the lung was already close to 100-fold lower than that of wild-type cells, consistent with our earlier study; this gap continued to increase until the wild-type-infected animals died. The mutant population in the lung continued to slowly grow, however, until those mice became ill, although it never reached the levels seen in wild-type infections despite the overt pulmonary symptoms. Also, although usv101Δ organisms eventually appeared in the brain, this was significantly delayed, as was dissemination to the spleen. For all three organs, the usv101Δ cell burden at the time of death was substantially lower than seen with wild-type fungi.

When harvesting organs for CFU analysis, we noticed that at the gross level, lungs collected from mice infected with wild-type fungi appeared more inflamed than those of mice infected with the usv101Δ mutant at 18 days postinfection, although by the time of death of the latter group (45 days), their lungs were dramatically enlarged and inflamed (Fig. 4C, insets). To pursue these observations, we examined pulmonary histology. Wild-type-infected animals at day 11 showed a uniform distribution of C. neoformans throughout the lung, most with large capsules, and mixed inflammation surrounding the airways, with predominantly polymorphonuclear leukocytes and some monocytic infiltrates (not shown). By day 18 (when WT mice succumbed to infection), increased inflammation and large numbers of fungi were evident throughout the lung (Fig. 4C, WT 18 days). In contrast to this disease progression in WT mice, usv101Δ strain-infected mouse lungs at day 11 showed overall fewer fungi (consistent with lower lung burden); these were centered on airways and surrounded by localized inflammation, with relatively few organisms observed peripherally in the lung (not shown). By day 18, increasing numbers of fungi could be seen, with a more diffuse distribution and inflammatory response but preservation of normal tissue appearance in some areas (Fig. 4C, Δ 18d). As infection progressed in usv101Δ strain-infected mice (day 32 [not shown]), the lesions became larger, extending to the periphery and occupying parenchymal spaces in the lung. Finally, by 6 to 7 weeks postinfection, when these animals succumbed to disease, most of the lung showed discrete lesions with concentrated organisms and exacerbated inflammation (Fig. 4C, Δ 45d). This is consistent with the respiratory distress of these animals.

When we probed the host response by profiling lung homogenates at various times after infection, we noted that neutrophil infiltration was significantly delayed in mice infected with the usv101Δ strain (Fig. 4D, red), compared to mice infected with the wild type (Fig. 4D, black). This observation was consistent with cytokine profiling of parallel lung samples (Fig. 4E), which showed similarly delayed induction of interleukin-1α (IL-1α), a proinflammatory cytokine that promotes neutrophil accumulation (26), as well as of neutrophil-attracting cytokines CCL2 (monocyte chemoattractant protein 1 [MCP-1]) and CCL3 (macrophage inflammatory protein 1α [MIP-1α]) (27).

To understand the striking phenotypes of cells lacking Usv101, we turned to its regulatory function. We have profiled virulence-associated traits (melanization, capsule size, capsule polysaccharide release, and pulmonary growth in mice) of 41 C. neoformans regulatory mutants in the same strain background (8). Of these strains, only the usv101Δ and ada2Δ strains showed defects in all of the features tested despite growing at rates similar to the wild type in multiple media (not shown). We previously combined network analysis and chromatin immunoprecipitation (ChIP) to define the regulatory network of Ada2 (7); here we determined how Usv101 regulates multiple virulence-associated traits.

We first examined the regulatory context of Usv101 together with two other key capsule TFs: Gat201 (28), and Rim101 (29). We mapped regulators that both act on these 3 TFs and whose deletion influences capsule thickness (see Text S1 in the supplemental material for details). The resulting network (Fig. 5A) shows that USV101 is positioned high in the capsule regulation cascade, regulated only by SWI6. This contrasts with RIM101, which has two regulatory inputs by capsule-implicated factors, and GAT201, which has six. Notably, USV101 does not appear to be affected by the cyclic AMP pathway; instead its influence converges with that of the cyclic AMP (cAMP) pathway at GAT201. Finally, USV101 is the only gene in this network whose deletion yields enlarged capsules. We also examined the kinetics of expression of the capsule-implicated TFs in this network (Fig. 5A, inset graphs). This showed that the variability in expression pattern occurred primarily at the early time points, with all mRNAs having their greatest expression at 24 h (see Text S1 for calculation of cAMP pathway activity).

To probe the regulatory function of Usv101 in detail, we next analyzed which capsule-implicated genes it regulates directly and how that target set changes during capsule induction. To do this we carried out RNA-seq experiments on the usv101Δ mutant and WT strains at three time points: before shifting to capsule-inducing conditions (0 min), 90 min after shifting (8), and 24 h after shifting. We identified the functional targets of Usv101 at each time point as those genes whose expression in the usv101Δ mutant was significantly different from their expression in WT (false discovery rate [FDR] of <0.02). We also carried out ChIP sequencing (ChIP-seq) experiments on cells expressing epitope-tagged Usv101 at both 0 and 90 min postinduction (8). We identified the direct functional targets of Usv101 at each time point as functional targets at that time point that were also ChIP positive at either 0 or 90 min postinduction. Notably, we were able to identify the same binding motif for Usv101 independently in both the ChIP-seq and the RNA-seq data. Using this motif, we scored all promoters according to their potential for binding Usv101 and considered those functional targets that scored in the top quintile as plausible direct functional targets. We then selected from the direct functional targets (identified by RNA-seq and either ChIP or binding potential) those whose corresponding deletion mutants had abnormal capsule. The result is shown in Fig. 5B.

Of the 19 direct, functional, capsule-involved targets shown in Fig. 5B, 13 are repressed by Usv101 (68%). When all direct, functional targets of Usv101 are considered, regardless of capsule involvement, there are fewer repressed targets under noninducing conditions (time zero of capsule induction), but the balance shifts sharply in favor of repression during the response to capsule-inducing conditions (see Fig. S3 in the supplemental material). These results support Usv101’s role as a transcriptional repressor that is central to capsule regulation.

The targets of Usv101 include both transcriptional regulators (circles in Fig. 5B) and downstream proteins with other biological functions. The first group includes 3 TFs whose absence results in minor capsule thickness abnormalities (FKH2, CLR4, and CLR1 [8]) and three others whose corresponding mutants have dramatically reduced capsule thickness (GAT201, RIM101, and SP1 [28, 30–32]) (Fig. 6A). These genes show increased expression in response to capsule induction (Fig. 6B, black bars), consistent with a role in furthering capsule growth. Deletion of USV101 increases the expression of all three (Fig. 6B, red bars), with the greatest effect on GAT201 at 90 min after induction. Overexpression of USV101 represses GAT201 slightly at 90 min but has little effect on RIM101 or SP1 (see Table S2 in the supplemental material), suggesting that its repressive effect on the latter two genes is already saturated at WT levels.

To better understand the relationships between Usv101 and Gat201, we constructed and characterized a gat201Δ usv101Δ double mutant. This strain was severely hypocapsular, like the gat201Δ single mutant (Fig. 6A), showing that capsule formation requires factors whose expression depends on the presence of Gat201. However, we know that expression of many other Usv101 targets is also perturbed in this mutant (Fig. 5A). To uncouple changes in GAT201 expression from the presence or absence of Usv101, we replaced the endogenous GAT201 promoter with four other promoters, which we know from RNA-seq are independent of Usv101 (see Materials and Methods). We then analyzed the resulting strains for capsule thickness (see Table S1B in the supplemental material) and gene expression (by RNA-seq). We found that reducing GAT201 expression, in the presence or absence of USV101, reduces capsule thickness (see Fig. S4 in the supplemental material), suggesting that increased GAT201 expression contributes to the capsule expansion of usv101Δ mutants.

The capsule thickness phenotype of the usv101Δ strain depends on the presence of GAT201, such that the gat201Δ usv101Δ double mutant matches the gat201Δ single mutant, but this relationship does not hold for all phenotypes. For example, like Chun et al (32), we found that unopsonized wild-type cells are poorly engulfed by host phagocytes, while gat201Δ mutants are taken up avidly; we also noted that deletion of USV101 has little effect on fungal engulfment in the wild-type background (Fig. 7). However, the gat201Δ usv101Δ double mutant, rather than resembling the gat201Δ single mutant, shows a phenotype midway between the low uptake of the wild-type and usv101Δ strains and the high uptake of the gat201Δ single mutant. (This may be because Usv101 and Gat201 regulate BLP1 in opposite directions [8, 32].) A similar pattern is seen when the cells are induced to form capsules before exposure to phagocytes (Fig. 7, DMEM), even though the induced capsule sizes of the double mutant and gat201Δ single mutant are quite similar (and distinct from that of the wild type [Fig. 6B]). Finally, the gat201Δ usv101Δ double mutant is more impaired than either single mutant in capsule shedding (not shown). In summary, for some phenotypes, USV101 deletion has an effect only in the presence of GAT201, for some it has an effect only in the absence of GAT201, and for some, the effects of the two deletions are relatively independent.

We next examined the relationship between Usv101 and its other 2 TF targets, Rim101 and Sp1, by deleting USV101 in rim101Δ or sp1Δ mutants, both of which are hypocapsular. In combination with rim101Δ or sp1Δ, unlike with gat201Δ, USV101 deletion increased capsule size (Fig. 6B; see Table S1B in the supplemental material). Consistent with the somewhat less severe capsule phenotype of the rim101Δ and sp1Δ mutants, this suggests that neither Rim101 nor Sp1 is strictly required for capsule formation or the capsule-expanding effects of USV101 deletion.

We wished to extend our analysis of Usv101 to understand the mutant phenotypes mechanistically. To do this, we returned to our analysis of its target genes and assessed those direct targets that are not themselves DNA-binding proteins (squares in Fig. 5B). Only one capsule-involved target was significantly repressed by Usv101 at all time points we examined (Fig. 5B, center). This gene, UXS1, encodes a UDP-xylose synthase that decarboxylates UDP-glucuronic acid to form UDP-xylose (33). Both of these compounds are nucleotide sugars, activated molecules that act as donors of monosaccharides for glycan synthesis, and both provide major components of the dominant capsule polysaccharide, glucuronoxylomannan (GXM). In the absence of Usv101 UXS1 would be derepressed, leading to an increase in UDP-xylose production. Consistent with this expectation, there is more xylose and less glucuronic acid in GXM of the usv101Δ strain (Fig. 8; see Table S3A in the supplemental material), compared to the wild type and the USV101-overexpressing strain. To directly test the effects of increased Uxs1 on capsule synthesis, we generated a strain in which UXS1 is driven by the actin promoter. As predicted by our model, the UXS1-overexpressing strain was hypercapsular (see Table S1B in the supplemental material), and its GXM showed increased xylose content (Fig. 8; see Table S3B in the supplemental material), both phenotypes similar to those of usv101Δ cells. It was only slightly impaired in virulence, however, suggesting that factors beyond capsule composition and thickness impact the virulence of the usv101Δ strain (see Fig. S5 in the supplemental material).

Our computational analyses allowed us to link the absence of USV101 to the observed changes in capsule size and composition via UXS1. We further probed our RNA-seq data for explanations of other characteristics we found to differ between wild-type and usv101Δ cells: melanin production, stress response, and cell wall thickness. Our expression analysis of genes implicated in melanin synthesis suggests that the defective melanization in usv101Δ cells (Fig. 2C) is likely due to significant downregulation of CTR1 expression compared to wild type (see Table S4 in the supplemental material). This gene encodes the high-affinity copper transporter Ctr1 (34), formerly termed Ctr2 (35). Because copper is required for the catalytic function of laccase, which makes melanin (34), cells lacking CTR1 do not produce this pigment. Furthermore, mice infected with C. neoformans lacking CTR1 show reduced organ burden in both lung and brain, suggesting that reduced expression of this gene may also contribute to the altered pathogenesis of the usv101Δ strain.

Another phenotype we noted in usv101Δ cells was increased resistance to salt. Saline stress has been shown to increase transcription of a variety of genes in S. cerevisiae, including multiple solute transporters (36). Consistent with these observations, multiple cryptococcal genes annotated as monosaccharide transporters were significantly upregulated in the mutant compared to the wild type (see Table S4 in the supplemental material), potentially explaining its increased salt resistance.

Finally, usv101Δ cells showed significant changes in their expression of genes related to cell wall synthesis (see Table S4 in the supplemental material), which is critical for the display of virulence factors such as capsule and melanin and for host interactions. In particular, we observed reduced expression of three genes (AGS1, CHS5, and SKN1) whose products act in synthesis of major cell wall polysaccharides (alpha-glucan, chitin, and beta-glucan, respectively). The reduction in each case was greater than 2-fold and was highly statistically significant (see Table S4). Expression of a glucanase was also significantly increased, suggesting heightened cell wall remodeling, perhaps secondary to perturbations of cell wall synthesis. Finally, the gene encoding Gas1, which mediates beta-glucan rearrangement, was upregulated. This may be a compensatory change, by which the mutant attempts to strengthen its wall in the face of defective synthesis of individual components. Similarly, an increase in CDA3 expression may reflect compensatory efforts to generate sufficient chitosan despite reduced synthesis of its precursor, chitin.

All animal studies were reviewed and approved by the Animal Studies Committee of the Washington University School of Medicine and conducted according to the National Institutes of Health guidelines for housing and care of laboratory animals.

All chemicals and PCR primers were from Sigma-Aldrich, reagents and enzymes were from Life Technologies, and DNA cleanup kits were from Qiagen unless otherwise noted.

All strains used in this study were constructed in the serotype A strain KN99α (37), as detailed in Text S1 in the supplemental material. Unless noted otherwise, cells were grown with continuous shaking (230 rpm) at 30°C in YPD medium or at 30°C on agar plates. As appropriate, media were supplemented with either 100 μg/ml of nourseothricin (Werner Bioagents) or 100 μg/ml of Geneticin.

To induce capsule formation, cells cultured overnight in YPD were collected by centrifugation, washed in Dulbecco’s modified Eagle’s medium (DMEM), and adjusted to 10⁶ cells/ml in DMEM preconditioned to 37°C and 5% CO₂ (capsule-inducing conditions) in 24-well plates. For inhibitor experiments, induction was carried out in the presence of either 20 μg/ml CHX or 200 μg/ml PHN as noted in Fig. 1. For CHX studies, viability was 63% after 8 h of drug treatment, decreasing to 38% at 24 h: if the drug was washed out at 8 h, viability recovered to 72% by 24 h of incubation.

Details of the assays used to characterize the engineered strains are provided in Text S1 in the supplemental material. These include measurement of capsule thickness after India ink staining, quantitation of shed capsule by enzyme-linked immunosorbent assay (ELISA), determination of capsule polysaccharide composition, and assessment of melanin formation and release.

Engulfment of cryptococcal cells by human THP-1 macrophages was measured by high-content imaging as reported in reference 24 and detailed in Text S1 in the supplemental material. Fungal survival within THP-1 cells was assessed by counting colony-forming units (CFU) released from lysed cells at various times (see Text S1).

To measure fungal transversal of the blood-brain barrier (BBB), we added washed fungi to in vitro model BBBs that were generated and assessed for transendothelial electrical resistance (TEER) as described in reference 42 and then monitored fungal transmigration by CFU (for details, see Text S1 in the supplemental material).

Groups of 4- to 6-week-old female A/Jcr mice (National Cancer Institute) were intranasally inoculated with 5 × 10⁴ fungal cells and monitored for long-term survival and organ burden as detailed in Text S1 in the supplemental material. The methods used for histology, flow cytometry, and cytokine analysis of harvested lungs are also provided in Text S1.

Details of cell growth for RNA isolation, the isolation procedure, and preparation of RNA-seq libraries are provided in Text S1 in the supplemental material. Three biological replicates of each deletion mutant were profiled. To control for batch effects, a set of three wild-type replicates was profiled with every batch of deletion mutants. The wild-type replicate set was carried through the experimental stages, from induction to sequencing, at the same time as its matched mutant replicate sets. For all RNA-seq samples, the mean and median sequencing depth were 5.0 and 4.7 million reads, respectively, and the interquartile range of sequencing depth was 4.1 to 5.3 million reads. Details of data analysis and quality control are provided in Text S1 in the supplemental material.

The methods used to construct a network depicting the capsule-implicated putative direct functional targets of Usv101, to estimate the Usv101 binding potential on each gene’s promoter, to construct the network of regulators of USV101, GAT204, and RIM101, and to characterize TF mRNA level and activity are detailed in Text S1 in the supplemental material.

All generated RNA-seq and ChIP-seq data have been submitted to the NCBI Gene Expression Omnibus (GEO; http://www.ncbi.nlm.nih.gov/geo/) under accession no. GSE69532 and GSE60398.