Cross-Recognition of Avian H5N1 Influenza Virus by Human Cytotoxic T-Lymphocyte Populations Directed to Human Influenza A Virus
ABSTRACT
MATERIALS AND METHODS
Cells.
Peptides.
Target cells.
T-cell clones.
In vitro expansion of influenza A virus-specific T-cell populations.
FATT-CTL assay.
Gamma interferon (IFN-γ) assay.
RESULTS
Comparison of amino acid sequences of known influenza A virus CTL epitopes.
The recognition of known CTL epitopes and their avian analogues.
Cross-recognition of the NP derived from influenza virus A/VN/1194/04.
Cross-recognition of BLCL infected with influenza virus A/VN/1194/04.
DISCUSSION




Sequence name | Variant sequence of the CTL epitope at the indicated HLA allelea | |||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
HLA-A1 PB1 (591-599) | HLA-A3 M1 (13-21) | HLA-A*0201 M1 (58-66) | HLA-B*3501 M1 (128-135) | HLA-A*0201 NS1 (122-130) | HLA-A*0201 NS1 (123-132) | HLA-B*44 NS1 (158-166) | HLA-A1 NP (44-52) | HLA-A*6801 NP (91-99) | HLA-B*1402 NP (146-154) | HLA-B*2705 NP (174-184) | HLA-A3 NP (188-198) | HLA-A3 NP (265-273) | HLA-B*3701 NP (339-347) | HLA-B*08 NP (380-388) | HLA-B*2702 NP (381-388) | HLA-B*2705 NP (383-391) | HLA-B*3501 NP (418-426) | |||||||||
Epitope | VSDGGPNLY | SIIPSGPLK | GILGFVFTL | ASCMGLIY | AIMDKNIIL | IMDKNIILKA | GEISPLPSL | CTELKLSDY | KTGGPIYKR | TTYQRTRAL | RRSGAAGAAVK | TMVMELVRMIK | ILRGSVAH | EDLRVLSFI | ELRSRYWAI | LRSRYWAI | SRYWAIRTR | LPFEKSTVM | ||||||||
Variant | ||||||||||||||||||||||||||
1 | I-------- | -V------- | -M------- | ------S- | -----T--- | ----T----- | ------H-- | --------H | -------R- | S-------- | ---------I- | ------I---- | -----I--- | -----S--- | ----K---- | ---K---- | -K------- | ----RA-I- | ||||||||
2 | -------P- | --V------ | --W------ | -----V--- | ----V----- | -------F- | --------Q | -----V--- | A-------- | -I------L-- | --A-------- | -----M--- | ----IS--- | D-LGK---K | -LGK---K | ------K-- | ----R--I- | |||||||||
3 | ------IP- | F-------- | -------T- | ------T--- | ---L----- | ------N-- | -----F-RG | AA------- | --F-------- | -----QI---- | V-------- | -----H--- | ----H--- | -------P- | ----RAAI- | |||||||||||
4 | G-------- | -T------- | -----TV-- | ----TV---- | --------I | ------T-- | --------G | A--H----- | Q----VI---- | --------K | ----RV-I- | |||||||||||||||
5 | -A------- | -L------- | -----A--- | ----A----- | -----S--- | ---F----- | AP------- | --------P | ----RA--- | |||||||||||||||||
6 | ATS------ | -T-----T- | T-----T--- | -------Y- | A-------V | GK---KWMI | ||||||||||||||||||||
7 | --VR----Q | -----D--- | ----D----- | A-------- | A-SQ----- | --H------ | ||||||||||||||||||||
8 | --VL----- | --V--T--- | -V--T----- | -----I--- | --------G | |||||||||||||||||||||
9 | S----T--- | ----T-S--- | ||||||||||||||||||||||||
10 | -----T-S- | --N------- | ||||||||||||||||||||||||
11 | ---N----- | -V--T-T--- | ||||||||||||||||||||||||
12 | --V--T-T- | -V----T--- | ||||||||||||||||||||||||
13 | --V----T- | ----T-L--- | ||||||||||||||||||||||||
14 | -----T-L- | ----I----- | ||||||||||||||||||||||||
15 | -----I--- | -V--TV---- | ||||||||||||||||||||||||
16 | --V--TV-- | ----T--W-- | ||||||||||||||||||||||||
17 | -----T--W | ----T----G |
Acknowledgments
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