MATERIALS AND METHODS
Cells and antibodies.
Quantification of cell-cell fusion.
Cell surface biotinylation and immunoprecipitation.
Native gel electrophoresis.
The HN proteins of HPIV2 and MuV can trigger noncognate F proteins.
Amino acid substitutions in region II variously affect F protein specificity.
Oligomeric stability of the HN protein does not correlate with F protein specificity.
Headless and truncated forms of the SV41 HN protein exhibit altered F protein specificity.
F-triggering activities of the truncated HN proteins are independent of their cell surface expression levels.
Replacement of region II or removal of the head domain converts the F protein specificity of the HPIV2 HN protein.
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