1 May 1996

Metronidazole and clarithromycin resistance in Helicobacter pylori determined by measuring MICs of antimicrobial agents in color indicator egg yolk agar in a miniwell format. The Gastrointestinal Physiology Working Group of Universidad Peruana Cayetano Heredia and the Johns Hopkins University

Abstract

Resistance of Helicobacter pylori to metronidazole often causes failure of commonly used combination drug treatment regimens. We determined the MICs of metronidazole and clarithromycin against 18 H. pylori strains from Peru using tetrazolium egg yolk (TEY) agar. The MIC results obtained by agar dilution with petri dishes were compared with the results found through a miniwell format. The results of the two protocols for measuring drug susceptibility differed by no more than 1 dilution in all cases. On TEY agar, bright-red H. pylori colonies were easy to identify against a yellow background. Sixty-one percent (11 of 18) of the strains were resistant to metronidazole (MIC, > or = 4 micrograms/ml) and 50% (9 of 18) were resistant to clarithromycin (MIC, > or = 0.125 micrograms/ml), whereas none (0 of 5) of the strains tested were resistant to tetracycline (MIC, > or = 1 micrograms/ml). Thus, the prevalence of metronidazole and clarithromycin resistance in Peru is higher than that in developed regions of the world. The miniwell plate with TEY agar allows easy H. pylori colony identification, requires about one-third less of the costly medium necessary for petri dish assaying, conserves space, and yields MICs equivalent to those with agar dilution in petri dishes.

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Published In

cover image Journal of Clinical Microbiology
Journal of Clinical Microbiology
Volume 34Number 5May 1996
Pages: 1232 - 1234
PubMed: 8727909

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Published online: 1 May 1996

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Authors

A Vasquez
Department of Pathology, Universidad Peruana Cayetano Heredia, Lima, Peru.
Y Valdez
Department of Pathology, Universidad Peruana Cayetano Heredia, Lima, Peru.
R H Gilman
Department of Pathology, Universidad Peruana Cayetano Heredia, Lima, Peru.
J J McDonald
Department of Pathology, Universidad Peruana Cayetano Heredia, Lima, Peru.
T U Westblom
Department of Pathology, Universidad Peruana Cayetano Heredia, Lima, Peru.
D Berg
Department of Pathology, Universidad Peruana Cayetano Heredia, Lima, Peru.
H Mayta
Department of Pathology, Universidad Peruana Cayetano Heredia, Lima, Peru.
V Gutierrez
Department of Pathology, Universidad Peruana Cayetano Heredia, Lima, Peru.

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