Research Article
1 October 1991

Identification of methicillin-resistant strains of staphylococci by polymerase chain reaction

Abstract

A simple and reliable method using a polymerase chain reaction (PCR) was devised to identify methicillin-resistant staphylococci. By using lysates of the strain to be tested as templates and 22-mer oligonucleotides as primers, a 533-bp region of mecA, the structural gene of a low-affinity penicillin-binding protein (PBP 2'), was amplified by PCR and detected by agarose gel electrophoresis. Results obtained by this method were compared with those obtained by broth microdilution MIC determination for 210 and 100 clinical isolates of Staphylococcus aureus and coagulase-negative staphylococci, respectively. Of 99 mecA-negative S. aureus isolates, 100% of the strains were methicillin susceptible and 98% of the strains were oxacillin susceptible. Three strains (3%) of 111 mecA-positive S. aureus isolates exhibited almost the same susceptibility to beta-lactams as the mecA-negative ones and did not produce detectable amounts of PBP 2' despite the presence of the mecA gene. One of them yielded typically methicillin-resistant variants at a low frequency with concomitant recovery of PBP 2' production. The mecA gene was also found in coagulase-negative Staphylococcus epidermidis, Staphylococcus haemolyticus, Staphylococcus sciuri, Staphylococcus saprophyticus, and Staphylococcus caprae and conferred resistance on most of the bacteria.

Formats available

You can view the full content in the following formats:

Information & Contributors

Information

Published In

cover image Journal of Clinical Microbiology
Journal of Clinical Microbiology
Volume 29Number 10October 1991
Pages: 2240 - 2244
PubMed: 1939577

History

Published online: 1 October 1991

Permissions

Request permissions for this article.

Contributors

Authors

K Murakami
Shionogi Research Laboratories, Shionogi and Co., Ltd., Osaka, Japan.
W Minamide
Shionogi Research Laboratories, Shionogi and Co., Ltd., Osaka, Japan.
K Wada
Shionogi Research Laboratories, Shionogi and Co., Ltd., Osaka, Japan.
E Nakamura
Shionogi Research Laboratories, Shionogi and Co., Ltd., Osaka, Japan.
H Teraoka
Shionogi Research Laboratories, Shionogi and Co., Ltd., Osaka, Japan.
S Watanabe
Shionogi Research Laboratories, Shionogi and Co., Ltd., Osaka, Japan.

Metrics & Citations

Metrics

Note:

  • For recently published articles, the TOTAL download count will appear as zero until a new month starts.
  • There is a 3- to 4-day delay in article usage, so article usage will not appear immediately after publication.
  • Citation counts come from the Crossref Cited by service.

Citations

If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. For an editable text file, please select Medlars format which will download as a .txt file. Simply select your manager software from the list below and click Download.

View Options

Figures and Media

Figures

Media

Tables

Share

Share

Share the article link

Share with email

Email a colleague

Share on social media

American Society for Microbiology ("ASM") is committed to maintaining your confidence and trust with respect to the information we collect from you on websites owned and operated by ASM ("ASM Web Sites") and other sources. This Privacy Policy sets forth the information we collect about you, how we use this information and the choices you have about how we use such information.
FIND OUT MORE about the privacy policy