Research Article
1 November 1988

Susceptibilities of 14 cell lines to bluetongue virus infection


The effect of bluetongue virus (BTV) infection was investigated in 14 cell lines. The cell lines included the following vertebrate cells: baby hamster kidney, African green monkey kidney (Vero), rabbit kidney, bovine kidney, canine kidney, bovine turbinate, bovine endothelium (CPAE), bighorn sheep tongue, equine dermis, gekko lung, rainbow trout gonad, and mouse fibroblast (L929); they also included the following invertebrate lines: mosquito and biting midge. Comparisons between the cell lines were made on the basis of time to observed cytopathic effects, titer in 50% tissue culture infectious doses, and titer in plaque-forming units. The CPAE cell line produced the highest BTV 50% tissue culture infectious dose of all cell lines tested. The Vero and L929 cells gave the most discrete plaques in plaque assays. Of the 14 cell lines tested, the CPAE cells were the most susceptible to both cell culture-adapted and animal source BTV. Bovine endothelial cells demonstrate significant potential as a cell culture system for BTV investigations.

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Published In

cover image Journal of Clinical Microbiology
Journal of Clinical Microbiology
Volume 26Number 11November 1988
Pages: 2324 - 2327
PubMed: 2853175


Published online: 1 November 1988


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S J Wechsler
Arthropod-Borne Animal Diseases Research Laboratory, U.S. Department of Agriculture, Laramie, Wyoming 82071.
L E McHolland
Arthropod-Borne Animal Diseases Research Laboratory, U.S. Department of Agriculture, Laramie, Wyoming 82071.

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