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Research Article
1 August 1993

Immunocytochemical analysis of poly-beta-hydroxybutyrate (PHB) synthase in Alcaligenes eutrophus H16: localization of the synthase enzyme at the surface of PHB granules

Abstract

Antibodies raised against the Alcaligenes eutrophus poly-beta-hydroxybutyrate (PHB) synthase polypeptide were used for immunocytochemical localization of the synthase enzyme in whole cells and purified PHB granules. The data presented demonstrate for the first time that the synthase enzyme is located on the surface of the PHB granule rather than being incorporated inside the granule during its formation. From these basic observations and data from the recent literature, a model of granule assembly is proposed.

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Published In

cover image Journal of Bacteriology
Journal of Bacteriology
Volume 175Number 16August 1993
Pages: 5289 - 5293
PubMed: 8349571

History

Published online: 1 August 1993

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Authors

T U Gerngross
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
P Reilly
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
J Stubbe
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
A J Sinskey
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
O P Peoples
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.

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