Free access
Research Article
1 July 1984

Biosynthesis of the sulfonolipid 2-amino-3-hydroxy-15-methylhexadecane-1-sulfonic acid in the gliding bacterium Cytophaga johnsonae


The biosynthesis of the sulfonolipid 2-amino-3-hydroxy-15-methylhexadecane-1-sulfonic acid (capnine) was studied by measuring the incorporation of possible precursors into the lipid by cells grown in the presence of precursors which were labeled with stable isotopes. Cells grown on yeast extract in the presence of DL-[3,3-2H2]serine contained 40.1 mol% of the protein-bound serine and 5.0 mol% of the protein-bound cysteine derived from the labeled serine. Cells grown in the presence of DL-[3,3-2H2]cystine acid contained 86.4 mol% of the molecules that had two deuteriums. These results are consistent with the possibility that biosynthesis of capnine occurs by the condensation of 13-methylmyristoyl-coenzyme A with cysteic acid, in a reaction analogous to the condensation of a palmitoyl-coenzyme A with serine to form 3-keto-sphinganine during the biosynthesis of sphingolipids.

Formats available

You can view the full content in the following formats:

Information & Contributors


Published In

cover image Journal of Bacteriology
Journal of Bacteriology
Volume 159Number 1July 1984
Pages: 42 - 46
PubMed: 6330048


Published online: 1 July 1984


Request permissions for this article.



Metrics & Citations


Note: There is a 3- to 4-day delay in article usage, so article usage will not appear immediately after publication.

Citation counts come from the Crossref Cited by service.


If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download.

View Options

Figures and Media






Share the article link

Share with email

Email a colleague

Share on social media

American Society for Microbiology ("ASM") is committed to maintaining your confidence and trust with respect to the information we collect from you on websites owned and operated by ASM ("ASM Web Sites") and other sources. This Privacy Policy sets forth the information we collect about you, how we use this information and the choices you have about how we use such information.
FIND OUT MORE about the privacy policy