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Research Article
1 April 1972

Histidine Uptake in Strains of Neurospora crassa with Normal and Mutant Transport Systems


Kinetic parameters for three systems of active histidine uptake by germinated conidia of Neurospora crassa have been measured. Each system appears to follow typical Michaelis-Menten kinetics when studied separately from the other systems. Under the conditions studied, the general amino acid transport system was found to account for the major portion of histidine uptake from low concentrations. Three types of transport mutants with altered growth inhibition patterns were selected in a histidine auxotroph. Growth of one mutant, type basa, could be inhibited by the addition of methionine to a histidine-supplemented medium, and another type, neua, could be inhibited by the addition of arginine. These mutants were shown to be lacking active histidine uptake by the basic amino acid and neutral amino acid transport systems, respectively. Another type of double mutant (his-3, neur) could be inhibited only by the addition of very high concentrations of methionine in the presence of arginine and histidine, and the mutation appeared to have altered the specificity of the neutral amino acid permease.

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Published In

cover image Journal of Bacteriology
Journal of Bacteriology
Volume 110Number 1April 1972
Pages: 313 - 320
PubMed: 4259665


Published online: 1 April 1972


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Department of Plant Sciences, Genetics Section, Texas A&M University, College Station, Texas 77843
Department of Agriculture and Fisheries, Leixlip, Co. Kildare, Ireland
Genetics and Cell Biology, University of Minnesota, St. Paul, Minnesota 55100

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