Research Article
1 October 1991

Chromosomal Integration and Expression of Two Bacterial α-Acetolactate Decarboxylase Genes in Brewer's Yeast

Abstract

A bacterial gene encoding α-acetolactate decarboxylase, isolated from Klebsiella terrigena or Enterobacter aerogenes, was expressed in brewer's yeast. The genes were expressed under either the yeast phosphoglycerokinase (PGK1) or the alcohol dehydrogenase (ADH1) promoter and were integrated by gene replacement by using cotransformation into the PGK1 or ADH1 locus, respectively, of a brewer's yeast. The expression level of the α-acetolactate decarboxylase gene of the PGK1 integrant strains was higher than that of the ADH1 integrants. Under pilot-scale brewing conditions, the α-acetolactate decarboxylase activity of the PGK1 integrant strains was sufficient to reduce the formation of diacetyl below the taste threshold value, and no lagering was needed. The brewing properties of the recombinant yeast strains were otherwise unaltered, and the quality (most importantly, the flavor) of the trial beers produced was as good as that of the control beer.

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Published In

cover image Applied and Environmental Microbiology
Applied and Environmental Microbiology
Volume 57Number 10October 1991
Pages: 2796 - 2803
PubMed: 16348559

History

Published online: 1 October 1991

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Authors

K. Blomqvist
Biotechnical Laboratory, Technical Research Centre of Finland (VTT), P.O. Box 202, SF-02151 Espoo, Finland
M.-L. Suihko
Biotechnical Laboratory, Technical Research Centre of Finland (VTT), P.O. Box 202, SF-02151 Espoo, Finland
J. Knowles
Biotechnical Laboratory, Technical Research Centre of Finland (VTT), P.O. Box 202, SF-02151 Espoo, Finland
M. Penttilä
Biotechnical Laboratory, Technical Research Centre of Finland (VTT), P.O. Box 202, SF-02151 Espoo, Finland

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