Glycine Betaine as a Direct Substrate for Methanogens (Methanococcoides spp.)
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
Sources of organisms.
Cultivation and media.
Analytical techniques.
RESULTS
Utilization of N-methylated glycines by Methanococcoides spp.

Substrate | Substrate consumed (mM) | Product formed (mM) | Protein formed (mg liter−1) | Growth yield (g [dry wt] mol methyl group−1) | ||
---|---|---|---|---|---|---|
Ammonium | DMG | Methane | ||||
Methylamine | 5.4 | 5.4 | 3.1 | 5.81 | 2.15 | |
Dimethylamine | 5.1 | 5.1 | 6.7 | 8.95 | 1.75 | |
Trimethylamine | 4.9 | 4.9 | 10.1 | 10.1 | 1.37 | |
Betaine | 5.4 | 5.2 | 3.6 | 10.7 | 3.96 |
Impact of trimethylamine on methanogenesis from glycine betaine by strain NM1.


Glycine betaine content in cells of Methanococcoides sp. strain NM1.
DISCUSSION
Glycine betaine, a new substrate for methanogenic pure cultures.
Incomplete degradation of glycine betaine.
Equation no. | Reaction | ΔG°′a (kJ/reaction) |
---|---|---|
1 | 4 (CH3)3N+CH2COO− + 2 H2O→4 (CH3)2NH+CH2COO− + 3 CH4 + CO2 | −721.7 |
2 | 4 (CH3)3N+CH2COO− + 6 H2O→4 H3N+CH2COO− + 9 CH4 + 3 CO2 | −992.8 |
3 | 2 (CH3)2NH+CH2COO− + 2 H2O→2 H3N+CH2COO− + 3 CH4 + CO2 | −135.5 |
4 | 4 (CH3)NH2+CH2COO− + 2 H2O→4 H3N+CH2COO− + 3 CH4 + CO2 | −157.7 |
5 | 4 (CH3)NH3+ + 2 H2O→4 NH4+ + 3 CH4 + CO2 | −172.1 |
Glycine betaine as a compatible solute in Methanococcoides sp. NM1.
ACKNOWLEDGMENTS
REFERENCES
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