RESULTS AND DISCUSSION
A gene cluster for isethionate degradation in Bacillus krulwichiae.
Biochemical characterization of IseD.
Growth of B. krulwichiae with isethionate as the sole carbon source.
MATERIALS AND METHODS
Gene syntheses and cloning.
Expression and purification of IseD.
Assay for isethionate oxidation by IseD.
Assay for sulfoacetaldehyde reduction by IseD.
LC-MS detection of sulfoacetaldehyde formation.
Growth of B. krulwichiae in isethionate medium.
Fuchsin assay for sulfite detection.
Protein identification by SDS-PAGE and mass spectrometry.
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