Penicillin-binding protein 4 overproduction increases beta-lactam resistance in Staphylococcus aureus
Abstract
The Staphylococcus aureus mutant strain PVI selected in vitro for methicillin resistance overexpressed penicillin-binding protein (PBP) 4. In the wild-type parent strain the pbp4 gene was separated by 419 nucleotides from a divergently transcribed abcA locus coding for an ATP-binding cassette transporter. The mutant PVI was shown to have a deletion in the pbp4-abcA promoter region that affected pbp4 transcription but not expression of abcA. Introduction of the pbp4 gene plus the mutant promoter region into different genetic backgrounds revealed that PBP 4 overproduction was sufficient to increase in vitro-acquired methicillin resistance independently of other chromosomal genes. The role of the AbcA transporter in methicillin resistance remained unknown.
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Information & Contributors
Information
Published In
Antimicrobial Agents and Chemotherapy
Volume 40 • Number 9 • September 1996
Pages: 2121 - 2125
PubMed: 8878592
History
Published online: 1 September 1996
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Citation
1996.
Penicillin-binding protein 4 overproduction increases beta-lactam resistance in Staphylococcus aureus. Antimicrob Agents Chemother
40:.
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