ABSTRACT

A pan-susceptible Salmonella enterica serovar Worthington isolate was detected in the stool of a man returning from Sri Lanka. Under ceftriaxone treatment, a third-generation cephalosporin (3GC)-resistant Salmonella Worthington was isolated after 8 days. Molecular analyses indicated that the two isolates were identical. However, the latter strain acquired a blaDHA-1-carrying IncFII plasmid probably from a Citrobacter amalonaticus isolate colonizing the gut. This is the first report of in vivo acquisition of plasmid-mediated resistance to 3GCs in S. enterica.

INTRODUCTION

Salmonella enterica isolates are important pathogens responsible for gastroenteritis but may also cause invasive infections where third-generation cephalosporins (3GCs) and fluoroquinolones are the treatments of choice (1). However, the rapid emergence of 3GC-resistant (3GC-R) strains in human and nonhuman settings presents a public health concern (2, 3). Most of these isolates produce extended-spectrum β-lactamases (ESBLs) or the plasmid-mediated AmpC (pAmpC) CMY-2 (47), whereas the DHA-1 pAmpC is rarely reported (812).
In this scenario, we note that cases of infection due to Salmonella enterica isolates where the organism undergoes in vivo acquisition of blaESBL or blapAmpC via mobile genetic elements (MGEs) from other species have not yet been reported. Here, we describe a clinical case in which this phenomenon was observed and define the characteristics of the recovered isolates.
In November 2018, a 77-year-old man presented with fever and respiratory symptoms 5 days after returning from a 2-month trip to Sri Lanka. His personal history included an IgG4 cholangiopathy, requiring immunosuppressive drugs, and hepatocellular carcinoma. Ceftriaxone was started empirically after sampling. Blood, sputum, and urine cultures did not reveal bacterial growth. PCR from a nasopharyngeal swab detected influenza B. Antimicrobial treatment was stopped after 6 days, and the patient was discharged. Two days before discharge, he passed loose stools revealing a pan-susceptible Salmonella spp. (strain 7101.67) in culture.
Five days after discharge, the patient was readmitted because of fatigue, nausea, persistent respiratory symptoms, and intermittent diarrhea. Another Salmonella spp. (strain 7102.58) grew in stool cultures. Ceftriaxone was restarted but, on detection of resistance toward 3GCs, it was switched to meropenem for 3 days and then streamlined to cefepime for 10 days (see Text S1 and Fig. S1 in the supplemental material for a full description of the clinical case).
In the routine clinical laboratory, stools were enriched in selenite broth and then plated on xylose lysine deoxycholate, MacConkey II, Rambach, and brilliant green agar plates (Oxoid). Bacterial identification (ID) was achieved at the genus level using matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) (Bruker). Species, subspecies, and serovars were determined using the White-Kauffmann-Le Minor scheme (13). Antimicrobial susceptibility tests (ASTs) were performed using the disk-diffusion method for all morphologically different colonies (14). Production of ESBLs was further investigated using the double-disk synergy test (DDST) (15), and MICs were obtained implementing both GNX2F and ESB1F Sensititre microdilution panels (ThermoFisher Scientific) and interpreted according to EUCAST criteria (16).
Based on the MIC values, Salmonella strain 7101.67 was confirmed as pan-susceptible, whereas Salmonella strain 7102.58 isolate was resistant to azithromycin, β-lactam–β-lactamase inhibitor combinations, and 3GCs but not to cefepime (Table 1). For Salmonella strain 7102.58, DDST results were also suspicious for the production of an inducible AmpC (see Fig. S2 in the supplemental material) (11). Both isolates were identified as Salmonella enterica subsp. enterica Worthington (13). In Western countries, this serovar is rarely detected in human or nonhuman settings (4, 5, 17). Specific data regarding Sri Lanka are scarce (18), but we emphasize that Salmonella Worthington has a high prevalence in India, where it is responsible for outbreaks in both hospital and community settings (1921).
TABLE 1
TABLE 1 Antimicrobial susceptibility tests for Salmonella, E. coli J53 transconjugant, and C. amalonaticus strains
AntibioticMICa (μg/ml) for:
S. Worthington 7101.67S. Worthington 7102.58 (collected 8 days later)E. coli J53E. coli J53 transconjugant (donor 7102.58)C. amalonaticus 4c
Ampicillin≤4, S≥32, R≤4, S≥32, R≥32, R
Piperacillin-tazobactam≤2, S64, R≤2, S≤2, S8, S
Ticarcillin-clavulanate≤8, S≥256, R≤8, S128, R≥256, R
Cephalothin≤4, NA≥32, NA≤4, NA≥32, NA≥32, NA
Cefoxitin≤2, NA≥128, NA≤2, NA≥128, NA≥128, NA
Ceftriaxone≤0.5, S32, R≤0.5, S2, I8, R
Cefotaxime≤0.125, S≥128, R≤0.125, S16, R32, R
Cefotaxime-clavulanate≤0.0625, NA≥128, NA≤0.0625, NA8, NA64, NA
Ceftazidime≤0.125, NA≥256, R≤0.125, S32, R128, R
Cefotaxime-clavulanate0.25, NA≥256, NA≤0.0625, NA16, NA≥256, NA
Cefepime≤0.5, S≤0.5, S≤0.5, S≤0.5, S≤0.5, S
Aztreonam≤1, S≥32, R≤1, S4, R16, R
Imipenem≤0.25, S≤0.25, S≤0.25, S≤0.25, S≤0.25, S
Meropenem≤0.5, S≤0.5, S≤0.5, S≤0.5, S≤0.5, S
Doripenem≤0.0625, S≤0.0625, S≤0.0625, S≤0.0625, S≤0.0625, S
Ertapenem≤0.125, S≤0.125, S≤0.125, S≤0.125, S≤0.125, S
Gentamicin≤0.5, S≤0.5, S≤0.5, S≤0.5, S≤0.5, S
Tobramycin≤0.5, S≤0.5, S≤0.5, S≤0.5, S4, I
Amikacin≤2, S≤2, S≤2, S≤2, S≤2, S
Ciprofloxacin≤0.125, NI0.5, NI≤0.125, S≤0.125, S1, R
Levofloxacin≤0.5, S≤0.5, S≤0.5, S≤0.5, S≤0.5, S
Azithromycinb8, NA≥256, NA
Doxycycline≤1, NA≤1, NA≤1, NA≤1, NA4, NA
Minocycline≤1, NA≤1, NA≤1, NA≤1, NA4, NA
Tigecycline≤0.125, NA≤0.125, NA≤0.125, S≤0.125, S1, R
Co-trimoxazole≤0.25, S1, S≤0.25, S≤0.25, S≥8, R
Colistin≤0.125, S≤0.125, S≤0.125, S≤0.125, S≤0.125, S
a
MICs were obtained with microdilution Sensititre panels (GNX2F and ESB1F) and interpreted according to EUCAST 2019 criteria (16). R, resistant; I, intermediate; S, susceptible; NI, not interpretable; NA, not available; –, not tested.
b
The azithromycin MIC value was obtained implementing the Etest (bioMérieux) method. EUCAST 2019 does not provide interpretative criteria, but strains with MICs of ≤16 μg/ml are defined as wild type (16).
c
MICs for six C. amalonaticus strains were available (see Fig. S5 in the supplemental material). Because all of them had the same phenotype, we show strain 4 as a representative (this strain also underwent WGS).
Presence of ESBL, pAmpC, and carbapenemase bla genes was rapidly investigated using the CT103XL microarray (Check-Points), which indicated that Salmonella strain 7101.67 did not possess bla genes, whereas Salmonella strain 7102.58 carried blaDHA-1 (22). Moreover, analysis of clonality using repetitive extragenic palindromic PCR (rep-PCR) showed that the two strains had identical band patterns (see Fig. S3 in the supplemental material) (23, 24). These findings supported the hypothesis that the first isolate acquired an MGE harboring blaDHA-1. To confirm this hypothesis, conjugation experiments were performed at 37°C with the Escherichia coli J53 recipient (rifampin resistant) and selection on MacConkey plates containing ampicillin and rifampin (both 50 μg/ml) (25). As a result, transconjugants possessing blaDHA-1 were obtained at a frequency of 5.2 × 10−6 (Table 1).
Several ESBL- or CMY-2-producing Salmonella Worthington strains have been isolated from humans (India) and food animals (United States) (4, 26), but those expressing DHA-1 had not been detected. To date, this inducible pAmpC has been reported only in Salmonella serovars Thompson, Enteritidis, Indiana, and Anatum (9, 10, 12, 27, 28).
For both Salmonella isolates, whole-genome sequencing (WGS) was performed using NovaSeq 6000 (Illumina) and MinION (Oxford Nanopore) (6, 25). Annotation was achieved using the NCBI Prokaryotic Genome Annotation Pipeline. Genomes were analyzed by employing the tools of the Center for Genomic Epidemiology (www.genomicepidemiology.org/). Results indicated that Salmonella isolate 7101.67 carried aac(6′)-Ia in the chromosome and qnrB19 on a 2.5-kb Col440I plasmid; four additional plasmids (not typeable) without antimicrobial resistance genes (ARGs) were also present. Conversely, Salmonella isolate 7102.58 possessed an additional 82-kb IncFII plasmid (named p7102_58-6) harboring qnrB4, sul1, dfrA17, mph(A), and blaDHA-1 ARGs. Both Salmonella strains were of sequence type (ST) 592, and they were genetically identical, as confirmed by cgMLST analysis (cgST 161578; see Fig. S4 in the supplemental material).
Worldwide, blaDHA-1 is detected mostly in Klebsiella pneumoniae and E. coli, and it is harbored by plasmids of different sizes and incompatibility groups (8, 29). In Switzerland, blaDHA-1 has been associated with plasmids R, FIIk, F, and HIB (3032). To our knowledge, only two IncFII plasmids carrying blaDHA-1 were previously reported: one (82 kb) in E. coli from the United Kingdom (GenBank accession no. MK048477) that was almost identical to p7102_58-6 and another one (111 kb) in an ST11 K. pneumoniae isolate from Malaysia (GenBank accession no. KY751925) (33). In all of these IncFII plasmids, the blaDHA-1 [along with qnrB4, sul1, and mph(A)] was part of a common large module (16.5 kb) that was similar to others already deposited and carried by different Inc group plasmids (Fig. 1). Such an element is included between two IS26, comprises a phage shock protein operon, and has likely been acquired through a transposition process (7).
FIG 1
FIG 1 Map of the IncFII-DHA-1 plasmid carried by Salmonella Worthington strain 7102.58. From center to outer band: GC skew, GC content, BLAST hits from plasmid containing the same region, and annotated codons. For each plasmid, species, host, region, and year of isolation, along with Inc group, are indicated. Ca, C. amalonaticus; Ec, E. coli; Kp, K. pneumoniae; Kv, Klebsiella variicola; Cs, Cronobacter sakazakii; SL, Salmonella Lamita; SA, Salmonella Anatum; NA, not available. Image was created using BRIG software with blast option “-culling_limit 1.”
In the effort to detect the natural blaDHA-1 donor carried at the gut level, stools (∼100 μg) were enriched overnight in Luria-Bertani broth supplemented with cefoxitin 12 μg/ml and vancomycin 1.5 μg/ml. A total of 100 μl was plated on ChromID ESBL (bioMérieux) and incubated overnight. Thirty resistant colonies underwent PCR to detect blaDHA (34); those testing positive were characterized (ID, AST, and WGS) as described above.
Unfortunately, patient stools collected during the hospitalizations (November/December 2018) were not available for further analyses. Only in April 2019 we could analyze such a sample, and a blaDHA-1-positive Citrobacter amalonaticus (strain 4) was detected (see Fig. S5 in the supplemental material; Table 1). No further blaDHA-1-positive species (e.g., E. coli or Klebsiella spp.) were found, with the exception of the blaDHA-1-positive Salmonella Worthington, which was still isolated by routine culture methods. WGS analysis indicated that C. amalonaticus strain 4 did not harbor ARGs in the chromosome, whereas blaDHA-1 was carried on an 82-kb IncFII plasmid identical to p7102_58-6 (Fig. 1).
To our knowledge, this is the first report of in vivo acquisition of plasmid-mediated resistance to 3GCs in a clinical isolate of S. enterica. Our best hypothesis is that under ceftriaxone selective pressure, the initial pan-susceptible Salmonella strain acquired the blaDHA-1-IncFII plasmid via conjugation. This MGE was carried by the C. amalonaticus isolate colonizing the intestinal tract and likely acquired in Sri Lanka together with the initial Salmonella Worthington. Our findings also emphasize that travel to the Indian subcontinent represents a serious risk of importing unusual multidrug-resistant Gram-negative bacteria that may serve as sources of life-threatening resistance genes that can be transferred to important human pathogens.

Accession numbers.

The following strains, together with their plasmids, were deposited in GenBank: Salmonella Worthington strain 7101.67 (GenBank accession no. CP039503CP039508), Salmonella Worthington strain 7102.58 (GenBank accession no. CP039509CP039515), and C. amalonaticus strain 4 (GenBank accession no. CP041362CP041363).

ACKNOWLEDGMENTS

This work was supported by the NRP-72, “National Research Program, Antimicrobial Resistance” (Swiss National Science Foundation; grant no. 177378 to A.E.). Partial support was also provided by NRP-72 grant no. 177368 to P.M.K.
We thank Maria V. Elzi, Carlo Casanova, Thomas Büdel (Institute for Infectious Diseases, University of Bern), and Stefanie Springe (Praxis im Monbijou, Bern) for support.

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REFERENCES

1.
Crump JA, Sjolund-Karlsson M, Gordon MA, Parry CM. 2015. Epidemiology, clinical presentation, laboratory diagnosis, antimicrobial resistance, and antimicrobial management of invasive Salmonella infections. Clin Microbiol Rev 28:901–937.
2.
European Food Safety Authority (EFSA) and European Centre for Disease Prevention and Control (ECDC). 2019. The European Union summary report on antimicrobial resistance in zoonotic and indicator bacteria from humans, animals and food in 2017. EFSA J 17:5598–5876.
3.
Centers for Disease Control and Prevention (CDC). 2013. Antibiotic resistance threats in the United States. CDC, Atlanta, GA. https://www.cdc.gov/drugresistance/pdf/ar-threats-2013-508.pdf.
4.
Elnekave E, Hong SL, Lim S, Hayer SS, Boxrud D, Taylor AJ, Lappi V, Noyes N, Johnson TJ, Rovira A, Davies P, Perez A, Alvarez J. 2019. Circulation of plasmids harboring resistance genes to quinolones and/or extended-spectrum cephalosporins in multiple Salmonella enterica serotypes from swine in the United States. Antimicrob Agents Chemother 63:e02602-18.
5.
González-Sanz R, Herrera-León S, de la Fuente M, Arroyo M, Echeita MA. 2009. Emergence of extended-spectrum β-lactamases and AmpC-type β-lactamases in human Salmonella isolated in Spain from 2001 to 2005. J Antimicrob Chemother 64:1181–1186.
6.
Clement M, Ramette A, Bernasconi OJ, Principe L, Luzzaro F, Endimiani A. 2018. Whole-genome sequence of the first extended-spectrum β-lactamase-producing strain of Salmonella enterica subsp. Microbiol Resour Announc 7:e00973-18.
7.
Carattoli A. 2003. Plasmid-mediated antimicrobial resistance in Salmonella enterica. Curr Issues Mol Biol 5:113–122.
8.
Hennequin C, Ravet V, Robin F. 2018. Plasmids carrying DHA-1 β-lactamases. Eur J Clin Microbiol Infect Dis 37:1197–1209.
9.
Yu F, Chen C, Chen Q, Yu X, Ding B, Yang L, Li Q, Qin Z, Parsons C, Zhang X, Zhang L, Qu D, Wang L, Pan J. 2012. Identification of transferable DHA-1 type AmpC β-lactamases and two mutations in quinolone resistance-determining regions of Salmonella enterica serovar Thompson. J Med Microbiol 61:460–462.
10.
Rayamajhi N, Jung BY, Cha SB, Shin MK, Kim A, Kang MS, Lee KM, Yoo HS. 2010. Antibiotic resistance patterns and detection of blaDHA-1 in Salmonella species isolates from chicken farms in South Korea. Appl Environ Microbiol 76:4760–4764.
11.
Meini S, Tascini C, Cei M, Sozio E, Rossolini GM. 2019. AmpC β-lactamase-producing Enterobacterales: what a clinician should know. Infection 47:363–375.
12.
Verdet C, Arlet G, Barnaud G, Lagrange PH, Philippon A. 2000. A novel integron in Salmonella enterica serovar Enteritidis, carrying the blaDHA-1 gene and its regulator gene ampR, originated from Morganella morganii. Antimicrob Agents Chemother 44:222–225.
13.
Grimont PAD, Weill FX. 2007. Antigenic formulae of the Salmonella serovars, 9th ed. WHO Collaborating Centre for Reference and Research on Salmonella. Paris, France.
14.
Clinical Laboratory Standards Institute. 2018. Performances standards for antimicrobial susceptibility testing. CLSI document M100-S28. Clinical and Laboratory Standards Institute, Wayne, PA.
15.
European Committee on Antimicrobial Susceptibility Testing. 2017. EUCAST guidelines for detection of resistance mechanisms and specific resistances of clinical and/or epidemiological importance. Version 2.0. http://www.eucast.org/fileadmin/src/media/PDFs/EUCAST_files/Resistance_mechanisms/EUCAST_detection_of_resistance_mechanisms_170711.pdf.
16.
European Committee on Antimicrobial Susceptibility Testing. 2019. Breakpoint tables for interpretation of MICs and zone diameters. Version 9.0. http://www.eucast.org/fileadmin/src/media/PDFs/EUCAST_files/Breakpoint_tables/v_9.0_Breakpoint_Tables.pdf.
17.
European Centre for Disease Prevention and Control. 2014. Annual epidemiological report 2014 – food- and waterborne diseases and zoonoses. European Centre for Disease Prevention and Control, Stockholm, Sweden.
18.
Tay MYF, Pathirage S, Chandrasekaran L, Wickramasuriya U, Sadeepanie N, Waidyarathna KDK, Liyanage LDC, Seow KLG, Hendriksen RS, Takeuchi MT, Schlundt J. 2019. Whole genome sequencing analysis of nontyphoidal Salmonella enterica of chicken meat and human origin under surveillance in Sri Lanka. Foodborne Pathog Dis 16:531–537.
19.
Kumar Y, Sharma A, Sehgal R, Kumar S. 2009. Distribution trends of Salmonella serovars in India (2001–2005). Trans R Soc Trop Med Hyg 103:390–394.
20.
Muley VA, Pol SS, Dohe VB, Nagdawane RP, Arjunwadkar VP, Pandit DP, Bharadwaj RS. 2004. Neonatal outbreak of Salmonella Worthington in a general hospital. Indian J Med Microbiol 22:51–53.
21.
Sinha S, Pazhani GP, Sen B, Niyogi SK. 2006. Molecular characterization of Salmonella enterica serotype Worthington isolated from childhood diarrhea cases in Kolkata, India. Jpn J Infect Dis 59:275–276.
22.
Bernasconi OJ, Principe L, Tinguely R, Karczmarek A, Perreten V, Luzzaro F, Endimiani A. 2017. Evaluation of a new commercial microarray platform for the simultaneous detection of β-lactamase and mcr-1 and mcr-2 genes in Enterobacteriaceae. J Clin Microbiol 55:3138–3141.
23.
Seiffert SN, Hilty M, Kronenberg A, Droz S, Perreten V, Endimiani A. 2013. Extended-spectrum cephalosporin-resistant Escherichia coli in community, specialized outpatient clinic and hospital settings in Switzerland. J Antimicrob Chemother 68:2249–2254.
24.
Pires J, Kuenzli E, Kasraian S, Tinguely R, Furrer H, Hilty M, Hatz C, Endimiani A. 2016. Polyclonal intestinal colonization with extended-spectrum cephalosporin-resistant Enterobacteriaceae upon traveling to India. Front Microbiol 7:1069.
25.
Luzzaro F, Clement M, Principe L, Viaggi V, Bernasconi OJ, Endimiani A. 2019. Characterization of the first ESBL-producing Shigella sonnei clinical isolate in Italy. J Glob Antimicrob Resist 17:58–59.
26.
Jain P, Roy S, Viswanathan R, Basu S, Singh AK, Dutta S. 2013. Concurrent and transferable resistance to extended-spectrum cephalosporins, monobactam and fluoroquinolone in a Salmonella enterica serovar Worthington blood isolate from a neonate in Kolkata, India. Int J Antimicrob Agents 41:494–495.
27.
Gaillot O, Clement C, Simonet M, Philippon A. 1997. Novel transferable β-lactam resistance with cephalosporinase characteristics in Salmonella enteritidis. J Antimicrob Chemother 39:85–87.
28.
Chiou CS, Hong YP, Liao YS, Wang YW, Tu YH, Chen BH, Chen YS. 2019. New multidrug-resistant Salmonella enterica serovar Anatum Clone, Taiwan, 2015–2017. Emerg Infect Dis 25:144–147.
29.
Hennequin C, Chlilek A, Beyrouthy R, Bonnet R, Robin F. 2018. Diversity of DHA-1-encoding plasmids in Klebsiella pneumoniae isolates from 16 French hospitals. J Antimicrob Chemother 73:2981–2989.
30.
Schluter A, Nordmann P, Bonnin RA, Millemann Y, Eikmeyer FG, Wibberg D, Puhler A, Poirel L. 2014. IncH-type plasmid harboring blaCTX-M-15, blaDHA-1, and qnrB4 genes recovered from animal isolates. Antimicrob Agents Chemother 58:3768–3773.
31.
Wohlwend N, Endimiani A, Francey T, Perreten V. 2015. Third-generation-cephalosporin-resistant Klebsiella pneumoniae isolates from humans and companion animals in Switzerland: spread of a DHA-producing sequence type 11 clone in a veterinary setting. Antimicrob Agents Chemother 59:2949–2955.
32.
Pilo P, Vogt D, Origgi FC, Endimiani A, Peterson S, Perreten V. 2015. First report of a multidrug-resistant Klebsiella pneumoniae of sequence type 11 causing sepsis in a free-ranging beaver (Castor fiber). Environ Microbiol Rep 7:351–353.
33.
Kim SY, Ko KS. 2019. Diverse plasmids harboring blaCTX-M-15 in Klebsiella pneumoniae ST11 isolates from several Asian countries. Microb Drug Resist 25:227–232.
34.
Dallenne C, Da Costa A, Decre D, Favier C, Arlet G. 2010. Development of a set of multiplex PCR assays for the detection of genes encoding important beta-lactamases in Enterobacteriaceae. J Antimicrob Chemother 65:490–495.

Information & Contributors

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Published In

cover image Antimicrobial Agents and Chemotherapy
Antimicrobial Agents and Chemotherapy
Volume 63Number 10October 2019
eLocator: 10.1128/aac.00992-19

History

Received: 15 May 2019
Returned for modification: 7 June 2019
Accepted: 24 July 2019
Published online: 23 September 2019

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Keywords

  1. AmpC
  2. DHA
  3. Worthington
  4. gut
  5. plasmid-mediated resistance
  6. treatment

Contributors

Authors

Mathieu Clément
Institute for Infectious Diseases, University of Bern, Bern, Switzerland
Institute for Infectious Diseases, University of Bern, Bern, Switzerland
Odette J. Bernasconi
Institute for Infectious Diseases, University of Bern, Bern, Switzerland
Guido Stirnimann
Department of Visceral Surgery and Medicine, Inselspital, University Hospital, University of Bern, Bern, Switzerland
Pascal M. Frey
Department of General Internal Medicine, Inselspital, University Hospital Bern, University of Bern, Bern, Switzerland
Guido V. Bloemberg
Swiss National Centre for Enteropathogenic Bacteria and Listeria (NENT), Institute for Food Safety and Hygiene, National Centre for Enteropathogenic Bacteria and Listeria (NENT), Vetsuisse Faculty, University of Zurich, Zurich, Switzerland
Institute for Infectious Diseases, University of Bern, Bern, Switzerland
Institute for Infectious Diseases, University of Bern, Bern, Switzerland

Notes

Address correspondence to Andrea Endimiani, [email protected].

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