Archaeal DNA Polymerase D but Not DNA Polymerase B Is Required for Genome Replication in Thermococcus kodakarensis
MATERIALS AND METHODS
T. kodakarensis strain construction and confirmation of genome structures.
Western blots with anti-Pol B and anti-Pol D polyclonal antibodies.
Sensitivity to UV light irradiation, MMS, and mitomycin C.
Cloning and mutagenesis of the gene (TK0001) encoding Pol B.
|Strain||Genotype or description||Reference|
|TR1||KW128 with trpE (TK0254) integrated upstream of TK0001||This work|
|TR2||TR1 with mutation in TK0001 encoding Pol B (D215A)||This work|
|TR3||TR1 with mutation in TK0001 encoding Pol B (V93Q)||This work|
|TR4||TR1 with mutations in TK0001 encoding Pol B (V93Q + D215A)||This work|
|TS742||TR1 with nonsense mutation in codon 3 of TK0001||This work|
|TS744||TR1 with 1.7-kbp deletion of the 5′ region of TK0001||This work|
|TS745||KW128 with TK0001 deleted and replaced by trpE (TK0254)||This work|
T. kodakarensis lacking Pol B grows normally.
T. kodakarensis lacking Pol B retains a wild-type mutation profile.
T. kodakarensis lacking Pol B has increased sensitivity to UV irradiation.
The entire 5,016-bp sequence of TK0001 is nonessential.
Is Pol D the replicative DNA polymerase?
What does Pol B do?
DNA replication systems in the three domains of life.
Information & Contributors
Metrics & Citations
Note: There is a 3- to 4-day delay in article usage, so article usage will not appear immediately after publication.
Citation counts come from the Crossref Cited by service.
If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download.
Subscribe and get full access to this article