11 June 2012

Draft Genome Sequence of “Candidatus Hamiltonella defensa,” an Endosymbiont of the Whitefly Bemisia tabaci

ABSTRACT

Candidatus Hamiltonella defensa” is a facultative endosymbiont of the whitefly Bemisia tabaci. Herein, we report the first draft genome sequence of “Candidatus Hamiltonella defensa” from the invasive Mediterranean cryptic species of the B. tabaci complex. The 1.84-Mbp genome sequence comprises 404 contigs and contains 1,806 predicted protein-coding genes.

GENOME ANNOUNCEMENT

“Candidatus Hamiltonella,” a gammaproteobacterium, is a maternally transmitted endosymbiont found sporadically in some sap-feeding insects, such as aphids and whiteflies. In some aphid individuals, the facultative symbiont “Candidatus Hamiltonella defensa” provides protection against parasitoid wasps by causing high mortality of wasp larvae (5) or induces the expression of host heat shock genes to increase host heat tolerance (6). In the whitefly Bemisia tabaci complex, “Candidatus Hamiltonella defensa” is found in only two important invasive cryptic species, Mediterranean and Middle East-Asia Minor 1 (formally referred to as the Q biotype and B biotype, respectively) (1, 2, 4, 8). Unlike “Candidatus Hamiltonella defensa” in aphids, the symbiont of B. tabaci shares bacteriocytes with the obligate symbiont “Candidatus Portiera aleyrodidarum” (4, 7). A recent study reported that the GroEL protein produced by “Candidatus Hamiltonella defensa” in Middle East-Asia Minor 1 cryptic species of B. tabaci can facilitate tomato yellow leaf curl virus transmission (3). However, as a whole, the functions of “Candidatus Hamiltonella defensa” in B. tabaci remain largely unknown. The genome sequence of “Candidatus Hamiltonella defensa” could provide useful information to understand the roles of this endosymbiont in the invasive B. tabaci.
Uncultured symbionts were isolated from the Mediterranean cryptic species of B. tabaci, and the whole genome of purified bacteria was amplified by multiple displacement amplification (MDA) using a Repli-g ultrafast minikit (Qiagen). Two paired-end libraries with average insert sizes of 200 bp and 2 kb were generated. Amplified DNA was sequenced using an Illumina HiSeq 2000 instrument at BGI-Shenzhen (Shenzhen, China). We obtained 240 Mb and 84 Mb of high-quality useful data from each library. The paired-end reads were de novo assembled using SOAPdenovo v1.05; and contigs were connected according to the 2-kb paired-end relationships. The coding sequences (CDSs) were predicted by Glimmer v3.0, and homologous comparison to the NCBI nonredundant database was performed by BLAST for function annotation. The rRNA and tRNA were identified using rRNAmmer and tRNAscan.
The results show that the draft genome of the B. tabaci symbiont “Candidatus Hamiltonella defensa” has approximately 1.84 Mbp with a G+C content of 40.3% and comprises 404 contigs (N50, 11.8 kb), which can be assembled into 372 scaffolds (N50, 14.2 kb).
A total of 1,806 genes and 38 tRNA genes are predicted within the genome of “Candidatus Hamiltonella defensa.” A total of 1,112 genes were mapped to pathways in the Kyoto Encyclopedia of Genes and Genomes, among which “Membrane Transport” (n = 301) has the highest percentage of genes, followed by “Replication and Repair” (n = 227), “Translation” (n = 136), “Carbohydrate Metabolism” (n = 128), “Metabolism of Cofactors and Vitamins” (n = 88), and “Amino Acid Metabolism” (n = 87). These annotations provide a valuable resource for investigation of the functions of “Candidatus Hamiltonella defensa” in the whitefly. Further genomic analyses and comparative studies will provide additional insight into this symbiont-host system.

Nucleotide sequence accession numbers.

This Whole Genome Shotgun project has been deposited at DDBJ/EMBL/GenBank under the accession number AJLH00000000. The version described in this paper is the first version, AJLH01000000.

ACKNOWLEDGMENTS

This work was supported by grants from the National Basic Research and Development Program of China (project 2009CB119203) and the National Natural Science Foundation of China (projects 31171848, 31021003, and 31101437).

REFERENCES

1.
Bing XL, Ruan YM, Rao Q, Wang XW, and Liu SS. Diversity of secondary endosymbionts among different putative species of the whitefly, Bemisia tabaci. Insect Sci., in press.
2.
Chu D et al. 2011. Further insights into the strange role of bacterial endosymbionts in whitefly, Bemisia tabaci: comparison of secondary symbionts from biotypes B and Q in China. Bull. Entomol. Res. 101:477–486.
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Gottlieb Y et al. 2010. The transmission efficiency of tomato yellow leaf curl virus by the whitefly Bemisia tabaci is correlated with the presence of a specific symbiotic bacterium species. J. Virol. 84:9310–9317.
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Gottlieb Y et al. 2008. Inherited intracellular ecosystem: symbiotic bacteria share bacteriocytes in whiteflies. FASEB J. 22:2591–2599.
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Oliver KM, Russell JA, Moran NA, and Hunter MS. 2003. Facultative bacterial symbionts in aphids confer resistance to parasitic wasps. Proc. Natl. Acad. Sci. U. S. A. 100:1803–1807.
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Russell JA and Moran NA. 2006. Costs and benefits of symbiont infection in aphids: variation among symbionts and across temperatures. Proc. R. Soc. B 273:603–610.
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Skaljac M, Zanic K, Ban SG, Kontsedalov S, and Ghanim M. 2010. Co-infection and localization of secondary symbionts in two whitefly species. BMC Microbiol. 10:142.
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Thierry M, Becker N, Hajri A, Reynaud B, and Lett Delatte JMH. 2011. Symbiont diversity and non-random hybridization among indigenous (Ms) and invasive (B) biotypes of Bemisia tabaci. Mol. Ecol. 20:2172–2187.

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Published In

cover image Journal of Bacteriology
Journal of Bacteriology
Volume 194Number 131 July 2012
Pages: 3558
PubMed: 22689243

History

Received: 5 April 2012
Accepted: 13 April 2012
Published online: 11 June 2012

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Contributors

Authors

Qiong Rao
Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou, China
Shuang Wang
BGI-Shenzhen, Shenzhen, China
Yun-Lin Su
Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou, China
Xiao-Li Bing
Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou, China
Shu-Sheng Liu
Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou, China
Xiao-Wei Wang
Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou, China

Notes

Address correspondence to Shu-Sheng Liu, [email protected], or Xiao-Wei Wang, [email protected].

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