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COVID 19 Special Collection

COVID-19 (SARS-CoV-2) Special Collection

Latest COVID-19 Articles

  • Commentary

    Application, Verification and Implementation of SARS-CoV-2 Serologic Assays with Emergency Use Authorization

    Interest continues to grow regarding the role of serologic assays for the detection of prior infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The U.S. Food and Drug Administration (FDA) has granted Emergency Use Authorization (EUA) status to many SARS-CoV-2 serologic assays. In this document, expert recommendations from clinical microbiologist members of the American Society for Microbiology (ASM) concerning detailed verification strategies for SARS-CoV-2 serologic assays with FDA EUA are provided, as are insights into assay limitations and reporting considerations for laboratories. Assessments concerning single and multi-antibody isotype detection assays, which may provide either differentiated or non-differentiated (i.e., total antibody) antibody class results, are addressed. Additional considerations prior to assay implementation are also discussed, including biosafety, quality control and proficiency testing strategies. As the landscape of SARS-CoV-2 serologic testing is rapidly changing, this document provides updated guidance for laboratorians on application of these assays.

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    Elitza S. Theel, Marc Roger Couturier, Laura Filkins, Elizabeth Palavecino, Stephanie Mitchell, Sheldon Campbell, Michael Pentella, Susan Butler-Wu, Kurt Jerke, Vaishali Dharmarha, Peggy McNult, Audrey N. Schuetz; on behalf of the American Society for Microbiology Clinical and Public Health Microbiology Committee, Subcommittee on Laboratory Practices

    Accepted Manuscript Posted 5 October 2020, JCM; Final Article Posted 17 December 2020

  • Research Article

    SARS-CoV-2 Seroprevalence among a Southern U.S. Population Indicates Limited Asymptomatic Spread under Physical Distancing Measures

    This study suggests limited but accelerating asymptomatic spread of SARS-CoV-2. Asymptomatic infections, like symptomatic infections, disproportionately affected vulnerable communities in this population, and seroprevalence was higher in African American participants than in White participants. The low, overall prevalence may reflect the success of shelter-in-place mandates at the time this study was performed and of maintaining effective physical distancing practices among suburban populations. Under these public health measures and aggressive case finding, outbreak clusters did not spread into the general population.

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    Amir Barzin, John L. Schmitz, Samuel Rosin, Rameet Sirpal, Martha Almond, Carole Robinette, Samantha Wells, Michael Hudgens, Andrew Olshan, Stephanie Deen, Patrick Krejci, Eugenia Quackenbush, Kevin Chronowski, Caleb Cornaby, Janette Goins, Linda Butler, Julia Aucoin, Kim Boyer, Janet Faulk, Devena Alston-Johnson, Cristen Page, Yijun Zhou, Lynne Fiscus, Blossom Damania, Dirk P. Dittmer, David B. Peden

    29 September 2020, mBio

  • Research Article

    S Protein-Reactive IgG and Memory B Cell Production after Human SARS-CoV-2 Infection Includes Broad Reactivity to the S2 Subunit

    The recent rapid worldwide spread of SARS-CoV-2 has established a pandemic of potentially serious disease in the highly susceptible human population. Key issues are whether humans have preexisting immune memory that provides some protection against SARS-CoV-2 and whether SARS-CoV-2 infection generates lasting immune protection against reinfection. Our analysis focused on pre- and postinfection IgG and IgG memory B cells (MBCs) reactive to SARS-CoV-2 proteins. Most importantly, we demonstrate that infection generates both IgG and IgG MBCs against the novel receptor binding domain and the conserved S2 subunit of the SARS-CoV-2 spike protein. Thus, even if antibody levels wane, long-lived MBCs remain to mediate rapid antibody production. Our study results also suggest that SARS-CoV-2 infection strengthens preexisting broad coronavirus protection through S2-reactive antibody and MBC formation.

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    Phuong Nguyen-Contant, A. Karim Embong, Preshetha Kanagaiah, Francisco A. Chaves, Hongmei Yang, Angela R. Branche, David J. Topham, Mark Y. Sangster

    25 September 2020, mBio

  • Commentary

    Antiviral Drug Discovery To Address the COVID-19 Pandemic

    The magnitude of the morbidity and mortality inflicted upon the global population in less than 1 year has driven the inescapable conclusion that the discovery and development of effective antiviral drugs for COVID-19 are urgent and should be prioritized. The antiviral drug discovery programs that emerged for HIV and hepatitis C virus have enabled technology and expertise to accelerate this process for SARS-CoV-2. The description of candidate lead inhibitors for the viral main protease (Mpro) exemplifies this accelerated approach and reminds us of the needs and opportunities for addressing this pandemic.

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    Douglas D. Richman

    25 September 2020, mBio

  • Research Article

    Rescue of SARS-CoV-2 from a Single Bacterial Artificial Chromosome

    The pandemic coronavirus (CoV) disease 2019 (COVID-19) caused by severe acute respiratory syndrome CoV-2 (SARS-CoV-2) is a major threat to global human health. To date, there are no approved prophylactics or therapeutics available for COVID-19. Reverse genetics is a powerful approach to understand factors involved in viral pathogenesis, antiviral screening, and vaccine development. In this study, we describe the feasibility of generating recombinant SARS-CoV-2 (rSARS-CoV-2) by transfection of a single bacterial artificial chromosome (BAC). Importantly, rSARS-CoV-2 possesses the same phenotype as the natural isolate in vitro and in vivo. This is the first description of a BAC-based reverse genetics system for SARS-CoV-2 and the first time that an rSARS-CoV-2 isolate has been shown to be phenotypically identical to a natural isolate in a validated animal model of SARS-CoV-2 infection. The BAC-based reverse genetics approach will facilitate the study of SARS-CoV-2 and the development of prophylactics and therapeutics for the treatment of COVID-19.

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    Chengjin Ye, Kevin Chiem, Jun-Gyu Park, Fatai Oladunni, Roy Nelson Platt II, Tim Anderson, Fernando Almazan, Juan Carlos de la Torre, Luis Martinez-Sobrido

    25 September 2020, mBio

  • Genome Sequences

    Coding-Complete Genome Sequences of Three SARS-CoV-2 Strains from Bangladesh

    We report the sequencing of three severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genomes from Bangladesh. We have identified a unique mutation (NSP2_V480I) in one of the sequenced genomes (isolate hCoV-19/Bangladesh/BCSIR-NILMRC-006/2020) compared to the sequences available in the Global Initiative on Sharing All Influenza Data (GISAID) database. The data from this analysis will contribute to advancing our understanding of the epidemiology of SARS-CoV-2 in Bangladesh as well as worldwide at the molecular level and will identify potential new targets for interventions.

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    Shahina Akter, Tanjina Akhtar Banu, Barna Goswami, Eshrar Osman, Mohammad Samir Uzzaman, M. Ahashan Habib, Iffat Jahan, Abu Sayeed Mohammad Mahmud, M. Murshed Hasan Sarker, M. Saddam Hossain, A. K. Mohammad Shamsuzzaman, Tasnim Nafisa, M. Maruf Ahmed Molla, Mahmuda Yeasmin, Asish Kumar Ghosh, Sheikh M. Selim Al Din, Utpal Chandra Ray, Salek Ahmed Sajib, Maqsud Hossain, M. Salim Khan

    24 September 2020, MRA

  • Genome Sequences

    Whole-Genome Sequence of SARS-CoV-2 Isolate Siena-1/2020

    The complete genome sequence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) isolate Siena-1/2020 was obtained by Nanopore sequencing, combining the direct RNA sequencing and amplicon sequencing approaches. The isolate belongs to the B1.1 lineage, which is prevalent in Europe, and contains a mutation in the spike protein coding sequence leading to the D614G amino acid change.

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    Maria Grazia Cusi, David Pinzauti, Claudia Gandolfo, Gabriele Anichini, Gianni Pozzi, Francesco Santoro

    24 September 2020, MRA

  • Commentary

    Favipiravir and the Need for Early Ambulatory Treatment of SARS-CoV2 Infection (COVID-19)

    It is becoming increasingly clear that SARS-CoV-2 like most human viral infections will require multiple drugs in combination to treat COVID-19 illness. In this issue of the Journal, Doi and colleagues successfully treated patients with early COVID-19 with favipiravir, an oral polymerase inhibitor to rapidly and substantially clear SARS-CoV-2 from nasal secretions irrespective if it was started relatively early or later within the first week of infection. These data support the concept that favipiravir could be paired with at least one more off-target antiviral agent (doxycycline, azithromycin, ivermectin) followed by corticosteroids and antithrombotics to prevent COVID-19 hospitalization and death in those over age 50 an or those with one or more comorbidities. Clinical trials and advanced practice should immediately pivot to combination/sequential drug therapy for ambulatory COVID-19 illness.

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    Peter A. McCullough [MD, MPH]

    Accepted Manuscript Posted 23 September 2020, AAC; Final Article Posted 17 November 2020

  • Letter to the Editor

    Measles-Mumps-Rubella Vaccine and COVID-19 Relationship

    I have read the article by Fidel and Noverr with great interest....

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    Öner Özdemir

    22 September 2020, mBio

  • Author Reply

    Reply to Özdemir, “Measles-Mumps-Rubella Vaccine and COVID-19 Relationship”

    First, we thank Dr. Özdemir for having enough interest in our paper (1) to submit the Letter to the Editor....

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    Paul L. Fidel Jr., Mairi C. Noverr

    22 September 2020, mBio

  • Research Article

    In Silico Structure-Based Repositioning of Approved Drugs for Spike Glycoprotein S2 Domain Fusion Peptide of SARS-CoV-2: Rationale from Molecular Dynamics and Binding Free Energy Calculations

    The present study provides the structural identification of the viable binding residues of the SARS-CoV-2 S2 fusion peptide region, which holds prime importance in the virus’s host cell fusion and entry mechanism. The classical molecular mechanics simulations were set on values that mimic physiological standards for a good approximation of the dynamic behavior of selected drugs in biological systems. The drug molecules screened and analyzed here have relevant antiviral properties, which are reported here and which might hint toward their utilization in the coronavirus disease 2019 (COVID-19) pandemic owing to their attributes of binding to the fusion protein binding region shown in this study.

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    Nishant Shekhar, Phulen Sarma, Manisha Prajapat, Pramod Avti, Hardeep Kaur, Anupam Raja, Harvinder Singh, Anusuya Bhattacharya, Saurabh Sharma, Subodh Kumar, Ajay Prakash, Bikash Medhi

    22 September 2020, mSystems

  • Research Article

    A prospective, randomized, open-label trial of early versus late favipiravir in hospitalized patients with COVID-19

    Favipiravir is an oral broad-spectrum inhibitor of viral RNA-dependent RNA polymerase that is approved for treatment of influenza in Japan. We conducted a prospective, randomized, open-label, multicenter trial of favipiravir for the treatment of COVID-19 at 25 hospitals across Japan. Eligible patients were adolescents and adults admitted with COVID-19 who were asymptomatic or mildly ill and had an Eastern Cooperative Oncology Group (ECOG) performance status of 0 or 1. Patients were randomly assigned at a 1:1 ratio to early or late favipiravir therapy (the same regimen starting on day 6 instead of day 1). The primary endpoint was viral clearance by day 6. The secondary endpoint was change in viral load by day 6. Exploratory endpoints included time to defervescence and resolution of symptoms. Eighty-nine patients were enrolled, of whom 69 were virologically evaluable. Viral clearance occurred within 6 days in 66.7% and 56.1% of the early and late treatment groups (adjusted hazard ratio [aHR], 1.42; 95% confidence interval [95% CI], 0.76–2.62). Of 30 patients who had a fever (≥37.5°C) on day 1, time to defervescence was 2.1 days and 3.2 days in the early and late treatment groups (aHR, 1.88; 95%CI, 0.81–4.35). During therapy, 84.1% developed transient hyperuricemia. Favipiravir did not significantly improve viral clearance as measured by RT-PCR by day 6 but was associated with numerical reduction in time to defervescence. Neither disease progression nor death occurred to any of the patients in either treatment group during the 28-day participation (Japan Registry of Clinical Trials jRCTs041190120).

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    Yohei Doi, Masaya Hibino, Ryota Hase, Michiko Yamamoto, Yu Kasamatsu, Masahiro Hirose, Yoshikazu Mutoh, Yoshito Homma, Masaki Terada, Taku Ogawa, Fumihiro Kashizaki, Toshihiko Yokoyama, Hayato Koba, Hideki Kasahara, Kazuhisa Yokota, Hideaki Kato, Junichi Yoshida, Toshiyuki Kita, Yasuyuki Kato, Tadashi Kamio, Nobuhiro Kodama, Yujiro Uchida, Nobuhiro Ikeda, Masahiro Shinoda, Atsushi Nakagawa, Hiroki Nakatsumi, Tomoya Horiguchi, Mitsunaga Iwata, Akifumi Matsuyama, Sumi Banno, Takenao Koseki, Mayumi Teramachi, Masami Miyata, Shigeru Tajima, Takahiro Maeki, Eri Nakayama, Satoshi Taniguchi, Chang Kweng Lim, Masayuki Saijo, Takumi Imai, Hisako Yoshida, Daijiro Kabata, Ayumi Shintani, Yukio Yuzawa, Masashi Kondo

    Accepted Manuscript Posted 21 September 2020, AAC; Final Article Posted 17 November 2020

  • Letter to the Editor

    Evaluation and Comparison of the Hologic Aptima SARS-CoV-2 and the CDC 2019 nCoV real-time RT-PCR Diagnostic Panel using a Four-Sample Pooling Approach

    To expand testing capacity during the SARS-CoV-2 pandemic, numerous molecular assays have been granted emergency use authorization (EUA) for testing individual patient samples but not for sample pooling.…

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    Stephanie L. Mitchell, Samantha E. Ventura

    Accepted Manuscript Posted 18 September 2020, JCM; Final Article Posted 18 November 2020

  • Research Article

    Impaired Cytotoxic CD8+ T Cell Response in Elderly COVID-19 Patients

    Cytotoxic T cells are responsible for the elimination of infected cells and are key players in the control of viruses. CD8+ T cells with an effector phenotype express cytotoxic molecules and are able to perform target cell killing. COVID-19 patients with a mild disease course were analyzed for the differentiation status and cytotoxic profile of CD8+ T cells. SARS-CoV-2 infection induced a vigorous cytotoxic CD8+ T cell response. However, this cytotoxic profile of T cells was not detected in COVID-19 patients over the age of 80 years. Thus, the absence of a cytotoxic response in elderly patients might be a possible reason for the more frequent severity of COVID-19 in this age group than in younger patients.

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    Jaana Westmeier, Krystallenia Paniskaki, Zehra Karaköse, Tanja Werner, Kathrin Sutter, Sebastian Dolff, Marvin Overbeck, Andreas Limmer, Jia Liu, Xin Zheng, Thorsten Brenner, Marc M. Berger, Oliver Witzke, Mirko Trilling, Mengji Lu, Dongliang Yang, Nina Babel, Timm Westhoff, Ulf Dittmer, Gennadiy Zelinskyy

    18 September 2020, mBio

  • Perspective

    The Social Distancing Imposed To Contain COVID-19 Can Affect Our Microbiome: a Double-Edged Sword in Human Health

    Hygienic measures imposed to control the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and contain COVID-19 have proven effective in controlling the pandemic. In this article, we argue that these measures could impact the human microbiome in two different and disparate ways, acting as a double-edged sword in human health. New lines of research have shown that the diversity of human intestinal and oropharyngeal microbiomes can shape pulmonary viral infection progression. Here, we suggest that the disruption in microbial sharing, as it is associated with dysbiosis (loss of bacterial diversity associated with an imbalance of the microbiota with deleterious consequences for the host), may worsen the prognosis of COVID-19 disease. In addition, social detachment can also decrease the rate of transmission of antibiotic-resistant bacteria. Therefore, it seems crucial to perform new studies combining the pandemic control of COVID-19 with the diversity of the human microbiome.

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    Célia P. F. Domingues, João S. Rebelo, Francisco Dionisio, Ana Botelho, Teresa Nogueira

    16 September 2020, mSphere

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Podcast from AAC

Watch 2020: The year of COVID-19. Guest: Jeanne Marrazzo. Hosted by AAC Editor in Chief Cesar A. Arias.

Podcast from JCM

Watch COVID-19: Clinical Labs in the Media Spotlight with Dr. Katherine Wu and Dr. Susan Butler-Wu. Hosted by Journal of Clinical Microbiology Editor in Chief, Dr. Alexander McAdam.

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